Spaced Motif analysis tool (SpaMo)

For further information on how to interpret these results or to get a copy of the MEME software please access http://meme.nbcr.net.

Primary Motif  |  Sequence Database  |  Secondary Databases  |  Spacing Analysis  |  Program information

Primary Motif

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Name Preview Significant Secondaries List
HGATAA (2.4e-254)
AGATAA
24 Ascl2 primary, M00698 (HEB), Tcfe2a secondary, M00176 (AP-4), M00277 (Lmo2), M01240 (BEN), M01288 (NeuroD), M00122 (USF), MA0267.1 (ACE2), Zic3 secondary, MA0210.1 (ara), MA0092.1 (Hand1::Tcfe2a), M00378 (Pax-4), M00414 (AREB6), M00072 (CP2), M00391 (UAY), M00344 (RAV1), Myf6 secondary, M00915 (AP-2), MA0103.1 (ZEB1)

Sequence Database

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Name Last Modified Loaded Too Short No Primary Too Similar Used
sequences Fri Apr 8 21:53:27 2011 2400 0 948 121 1331

Secondary Databases

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Name Last Modified Number of Motifs Motifs Significant Motifs Redundant
JASPAR CORE 2009 Tue Mar 29 11:25:11 2011 476 5 3
transfac matrix Tue Mar 29 11:25:11 2011 1226 15 21
uniprobe mouse Tue Mar 29 11:25:11 2011 386 4 4

Spacings of "Ascl2 primary" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: Ascl2 primary
AGATAA
CTCAGCAGCTGCTCCTG
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
2.3e-07 7 17  
P-value Gap #  
2.8e-15 5 24  
0.0023 4 13  
0.019 6 12  

Total sequences with primary and secondary motif

893

Motif Database

uniprobe mouse

Secondary motifs with similar spacings

Similar Secondary: M00804 (E2A)
Same Strand
Opposite Strand
P-value Gap #  
0.0012 2 13  
P-value Gap #  
1.3e-14 8 23  
0.011 7 12  

Total sequences with primary and secondary motif

833

Alignment by most significant spacings

Best Similar
Secondary
CTCAGCAGCTGCTCCTG
This Similar
Secondary
    CCACCTGTCTCAGG
Similar Secondary: M00712 (myogenin)
Same Strand
Opposite Strand
P-value Gap #  
9.5e-14 8 21  
0.0016 7 12  
P-value Gap #  
0.0016 6 12  

Total sequences with primary and secondary motif

720

Alignment by most significant spacings

Best Similar
Secondary
CAGGAGCAGCTGCTGAG
This Similar
Secondary
    GGCAGGTG
Similar Secondary: M00184 (MyoD)
Same Strand
Opposite Strand
P-value Gap #  
0.0011 6 13  
P-value Gap #  
1.8e-13 8 22  
1.1e-05 7 15  

Total sequences with primary and secondary motif

851

Alignment by most significant spacings

Best Similar
Secondary
CTCAGCAGCTGCTCCTG
This Similar
Secondary
   AGCACCTGTC
Similar Secondary: Myf6 primary
Same Strand
Opposite Strand
P-value Gap #  
1.4e-12 6 21  
0.0068 5 12  
P-value Gap #  
7e-06 6 15  

Total sequences with primary and secondary motif

811

Alignment by most significant spacings

Best Similar
Secondary
CAGGAGCAGCTGCTGAG
This Similar
Secondary
GAAGAACAGGTGTCCG
Similar Secondary: Tcfe2a primary
Same Strand
Opposite Strand
P-value Gap #  
4.3e-11 7 18  
3.1e-05 6 13  
0.0042 8 11  
P-value Gap #  
0.042 7 10  

Total sequences with primary and secondary motif

631

Alignment by most significant spacings

Best Similar
Secondary
CAGGAGCAGCTGCTGAG
This Similar
Secondary
 ATCCACAGGTGCGAAAA
Similar Secondary: M00001 (MyoD)
Same Strand
Opposite Strand
P-value Gap #  
5.1e-11 5 17  

Total sequences with primary and secondary motif

521

Alignment by most significant spacings

Best Similar
Secondary
CAGGAGCAGCTGCTGAG
This Similar
Secondary
   GAACAGGTGTTG
Similar Secondary: M00929 (MyoD)
Same Strand
Opposite Strand
P-value Gap #  
5.1e-10 5 20  
7.6e-06 4 16  
P-value Gap #  
7.5e-07 4 17  

Total sequences with primary and secondary motif

943

Alignment by most significant spacings

Best Similar
Secondary
CAGGAGCAGCTGCTGAG
This Similar
Secondary
CCGAAGCAGGTGGCGAAG
Similar Secondary: M00973 (E2A)
Same Strand
Opposite Strand
P-value Gap #  
2.8e-06 6 16  
0.00028 5 14  
P-value Gap #  
1.9e-09 8 19  
2.8e-06 7 16  
0.021 9 12  

Total sequences with primary and secondary motif

913

Alignment by most significant spacings

Best Similar
Secondary
CTCAGCAGCTGCTCCTG
This Similar
Secondary
     CACCTGTC
Similar Secondary: M00644 (LBP-1)
Same Strand
Opposite Strand
P-value Gap #  
2.7e-08 7 16  
P-value Gap #  
5e-06 8 14  

Total sequences with primary and secondary motif

672

Alignment by most significant spacings

Best Similar
Secondary
CAGGAGCAGCTGCTGAG
This Similar
Secondary
      CAGCTGC
Similar Secondary: M00693 (E12)
Same Strand
Opposite Strand
P-value Gap #  
2.5e-07 5 15  
0.00051 4 12  
P-value Gap #  
4.3e-05 6 13  

Total sequences with primary and secondary motif

635

Alignment by most significant spacings

Best Similar
Secondary
CAGGAGCAGCTGCTGAG
This Similar
Secondary
    GGCAGGTGCCG
Similar Secondary: M00002 (E47)
Same Strand
Opposite Strand
P-value Gap #  
1.4e-06 5 13  

Total sequences with primary and secondary motif

472

Alignment by most significant spacings

Best Similar
Secondary
CAGGAGCAGCTGCTGAG
This Similar
Secondary
  ACGGCAGGTGTCCGC
Similar Secondary: M00071 (E47)
Same Strand
Opposite Strand
P-value Gap #  
1.9e-06 5 13  
P-value Gap #  
0.0053 6 10  

Total sequences with primary and secondary motif

492

Alignment by most significant spacings

Best Similar
Secondary
CAGGAGCAGCTGCTGAG
This Similar
Secondary
 AATAACAGGTGTTCAC
Similar Secondary: M00066 (Tal-1alpha:E47)
Same Strand
Opposite Strand
P-value Gap #  
0.00027 6 11  
0.0036 5 10  
P-value Gap #  
0.043 6 9  

Total sequences with primary and secondary motif

476

Alignment by most significant spacings

Best Similar
Secondary
CAGGAGCAGCTGCTGAG
This Similar
Secondary
 TCGAACAGATGGTGAC
Similar Secondary: M00065 (Tal-1beta:E47)
Same Strand
Opposite Strand
P-value Gap #  
0.021 6 9  
P-value Gap #  
0.021 6 9  

Total sequences with primary and secondary motif

435

Alignment by most significant spacings

Best Similar
Secondary
CAGGAGCAGCTGCTGAG
This Similar
Secondary
 CTGAACAGATGGTCGG

Spacings of "M00698 (HEB)" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: M00698 (HEB)
AGATAA
GCCAGCTG
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
4.4e-11 8 19  
0.02 7 11  
P-value Gap #  
9.4e-09 6 17  

Total sequences with primary and secondary motif

740

Motif Database

transfac matrix

Secondary motifs with similar spacings

Similar Secondary: MA0048.1 (NHLH1)
Same Strand
Opposite Strand
P-value Gap #  
9.7e-10 6 16  

Total sequences with primary and secondary motif

529

Alignment by most significant spacings

Best Similar
Secondary
 GCCAGCTG
This Similar
Secondary
GCGCAGCTGCGT
Similar Secondary: M00993 (TAL1)
Same Strand
Opposite Strand
P-value Gap #  
1.8e-08 6 16  
P-value Gap #  
1.8e-08 6 16  

Total sequences with primary and secondary motif

640

Alignment by most significant spacings

Best Similar
Secondary
GCCAGCTG
This Similar
Secondary
TCCATCTGCT
Similar Secondary: M00927 (AP-4)
Same Strand
Opposite Strand
P-value Gap #  
3.6e-08 7 19  
P-value Gap #  
3.6e-06 6 17  

Total sequences with primary and secondary motif

1073

Alignment by most significant spacings

Best Similar
Secondary
GCCAGCTG
This Similar
Secondary
ACCAGCTGC

Spacings of "Tcfe2a secondary" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: Tcfe2a secondary
AGATAA
AAGGCCAGATGGTCCGG
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
2.2e-06 6 15  
P-value Gap #  
6.8e-11 8 19  

Total sequences with primary and secondary motif

758

Motif Database

uniprobe mouse

Secondary motifs with similar spacings

Similar Secondary: MA0091.1 (TAL1::TCF3)
Same Strand
Opposite Strand
P-value Gap #  
2.7e-06 6 12  

Total sequences with primary and secondary motif

402

Alignment by most significant spacings

Best Similar
Secondary
CCGGACCATCTGGCCTT
This Similar
Secondary
  CGACCATCTGTT
Similar Secondary: M00068 (HEN1)
Same Strand
Opposite Strand
P-value Gap #  
0.007 2 8  

Total sequences with primary and secondary motif

258

Alignment by most significant spacings

Best Similar
Secondary
  CCGGACCATCTGGCCTT
This Similar
Secondary
ATGGGGCGCAGCTGCGCCCCTC

Spacings of "M00176 (AP-4)" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: M00176 (AP-4)
AGATAA
CACAGCTGGT
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
6.5e-09 7 17  

Total sequences with primary and secondary motif

718

Motif Database

transfac matrix

Secondary motifs with similar spacings

Similar Secondary: M01287 (Neuro)
Same Strand
Opposite Strand
P-value Gap #  
3e-08 8 18  
P-value Gap #  
3.4e-07 8 17  

Total sequences with primary and secondary motif

941

Alignment by most significant spacings

Best Similar
Secondary
CACAGCTGGT
This Similar
Secondary
  CAGCTG
Similar Secondary: M00175 (AP-4)
Same Strand
Opposite Strand
P-value Gap #  
2.9e-07 8 17  

Total sequences with primary and secondary motif

919

Alignment by most significant spacings

Best Similar
Secondary
CACAGCTGGT
This Similar
Secondary
CTCAGCTGGT

Spacings of "M00277 (Lmo2)" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: M00277 (Lmo2)
AGATAA
CGCCAGGTGCAG
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
2.1e-08 7 18  
0.00026 6 14  
P-value Gap #  
2.1e-08 5 18  
0.00026 4 14  

Total sequences with primary and secondary motif

896

Motif Database

transfac matrix

Secondary motifs with similar spacings

Similar Secondary: M01034 (Ebox)
Same Strand
Opposite Strand
P-value Gap #  
0.00016 6 15  
P-value Gap #  
1.8e-07 8 18  

Total sequences with primary and secondary motif

1034

Alignment by most significant spacings

Best Similar
Secondary
CTGCACCTGGCG
This Similar
Secondary
  CCACGTGACC

Spacings of "M01240 (BEN)" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: M01240 (BEN)
AGATAA
CAGCGACC
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
2.6e-07 8 17  

Total sequences with primary and secondary motif

912

Motif Database

transfac matrix

Spacings of "M01288 (NeuroD)" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: M01288 (NeuroD)
AGATAA
CTGCTGCTGTGC
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
7.3e-07 5 18  
P-value Gap #  
6.8e-06 5 17  
0.0039 4 14  

Total sequences with primary and secondary motif

1096

Motif Database

transfac matrix

Spacings of "M00122 (USF)" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: M00122 (USF)
AGATAA
AAATCACGTGATAT
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
2.6e-06 6 14  

Total sequences with primary and secondary motif

626

Motif Database

transfac matrix

Secondary motifs with similar spacings

Similar Secondary: M00796 (USF)
Same Strand
Opposite Strand
P-value Gap #  
0.013 6 11  

Total sequences with primary and secondary motif

699

Alignment by most significant spacings

Best Similar
Secondary
ATATCACGTGATTT
This Similar
Secondary
 GACCACGTGACA

Spacings of "MA0267.1 (ACE2)" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: MA0267.1 (ACE2)
AGATAA
ACCAGCA
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
3.2e-05 6 16  

Total sequences with primary and secondary motif

1093

Motif Database

JASPAR CORE 2009

Secondary motifs with similar spacings

Similar Secondary: MA0402.1 (SWI5)
Same Strand
Opposite Strand
P-value Gap #  
0.0018 6 13  

Total sequences with primary and secondary motif

895

Alignment by most significant spacings

Best Similar
Secondary
TGCTGGT
This Similar
Secondary
TGCTGGTT

Spacings of "Zic3 secondary" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: Zic3 secondary
AGATAA
GAGCACAGCAGGACA
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
4.3e-05 5 14  

Total sequences with primary and secondary motif

777

Motif Database

uniprobe mouse

Secondary motifs with similar spacings

Similar Secondary: Zic2 secondary
Same Strand
Opposite Strand
P-value Gap #  
6.4e-05 5 14  

Total sequences with primary and secondary motif

802

Alignment by most significant spacings

Best Similar
Secondary
GAGCACAGCAGGACA
This Similar
Secondary
CCACACAGCAGGAGA
Similar Secondary: Zic1 secondary
Same Strand
Opposite Strand
P-value Gap #  
7e-05 5 14  

Total sequences with primary and secondary motif

807

Alignment by most significant spacings

Best Similar
Secondary
GAGCACAGCAGGACA
This Similar
Secondary
CCACACAGCAGGAGA

Spacings of "MA0210.1 (ara)" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: MA0210.1 (ara)
AGATAA
TAACA
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
0.00012 5 15  

Total sequences with primary and secondary motif

1033

Motif Database

JASPAR CORE 2009

Spacings of "MA0092.1 (Hand1::Tcfe2a)" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: MA0092.1 (Hand1::Tcfe2a)
AGATAA
GGTCTGGCAT
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
0.00036 4 14  

Total sequences with primary and secondary motif

924

Motif Database

JASPAR CORE 2009

Spacings of "M00378 (Pax-4)" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: M00378 (Pax-4)
AGATAA
AATCCCCACCCG
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
0.00068 7 15  

Total sequences with primary and secondary motif

1163

Motif Database

transfac matrix

Spacings of "M00414 (AREB6)" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: M00414 (AREB6)
AGATAA
CTGCACCTGTGC
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
0.0013 6 9  

Total sequences with primary and secondary motif

311

Motif Database

transfac matrix

Spacings of "M00072 (CP2)" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: M00072 (CP2)
AGATAA
GCACAACCCAG
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
0.0017 0 10  

Total sequences with primary and secondary motif

435

Motif Database

transfac matrix

Spacings of "M00391 (UAY)" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: M00391 (UAY)
AGATAA
ATCGCAGGAGGCCGAC
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
0.0023 19 5  

Total sequences with primary and secondary motif

40

Motif Database

transfac matrix

Spacings of "M00344 (RAV1)" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: M00344 (RAV1)
AGATAA
ATCACCTGAGGC
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
0.0028 7 14  

Total sequences with primary and secondary motif

1107

Motif Database

transfac matrix

Spacings of "Myf6 secondary" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: Myf6 secondary
AGATAA
AGCAACAGCCGCACC
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
0.0036 6 14  

Total sequences with primary and secondary motif

1090

Motif Database

uniprobe mouse

Spacings of "M00915 (AP-2)" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: M00915 (AP-2)
AGATAA
CGGCCCCCAGGCC
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
0.005 1 13  

Total sequences with primary and secondary motif

943

Motif Database

transfac matrix

Spacings of "MA0103.1 (ZEB1)" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: MA0103.1 (ZEB1)
AGATAA
CACCTG
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
0.0051 7 11  

Total sequences with primary and secondary motif

652

Motif Database

JASPAR CORE 2009

Secondary motifs with similar spacings

Similar Secondary: M00073 (deltaEF1)
Same Strand
Opposite Strand
P-value Gap #  
0.02 7 11  

Total sequences with primary and secondary motif

745

Alignment by most significant spacings

Best Similar
Secondary
   CACCTG
This Similar
Secondary
ACTCACCTGAA

Spacings of "M01090 (MAD)" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: M01090 (MAD)
AGATAA
GCGACGAC
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
0.0051 4 12  

Total sequences with primary and secondary motif

806

Motif Database

transfac matrix

Spacings of "M00044 (Sn)" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: M00044 (Sn)
AGATAA
ACCACCTGTTTACA
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
0.015 2 11  

Total sequences with primary and secondary motif

688

Motif Database

transfac matrix

Spacings of "M01139 (LMAF)" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: M01139 (LMAF)
AGATAA
GGTCAGCAG
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
0.019 2 10  

Total sequences with primary and secondary motif

575

Motif Database

transfac matrix

Spacings of "MA0055.1 (Myf)" relative to "HGATAA (2.4e-254)"

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Primary: HGATAA (2.4e-254) Secondary: MA0055.1 (Myf)
AGATAA
CAGCAGCTGCTG
Motif Spacing Histogram   Significant Motif Spacings  
Upstream Downstream   Upstream Downstream Other Details
Same Strand
Opposite Strand
P-value Gap #  
0.03 5 9  

Total sequences with primary and secondary motif

449

Motif Database

JASPAR CORE 2009

Secondary motifs with similar spacings

Similar Secondary: M01302 (MYF)
Same Strand
Opposite Strand
P-value Gap #  
0.03 5 9  

Total sequences with primary and secondary motif

449

Alignment by most significant spacings

Best Similar
Secondary
CAGCAGCTGCTG
This Similar
Secondary
CAGCAGCTGCTG
Previous Top
SpaMo version
4.6.1 (Release date: Mon Mar 21 13:41:45 EST 2011)
Reference
"Inferring transcription factor complexes from ChIP-seq data", Tom Whitington, Martin C. Frith, James Johnson, and Timothy L. Bailey, submitted for publication.
Command line summary

Result calculation took 3 minutes 53 seconds
Note that the random number generator was initilized with a seed of 1 so you need "-numgen 1" in the list of arguments to replicate the experiment.
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