## net.sf.picard.metrics.StringHeader # net.sf.picard.analysis.CollectAlignmentSummaryMetrics INPUT=can_0_A.bam OUTPUT=can.metr.txt REFERENCE_SEQUENCE=aa.fasta VALIDATION_STRINGENCY=SILENT ASSUME_SORTED=true MAX_INSERT_SIZE=100000 ADAPTER_SEQUENCE=[AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT, AGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG, AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT, AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTG, AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT, AGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNNNATCTCGTATGCCGTCTTCTGCTTG] IS_BISULFITE_SEQUENCED=false TMP_DIR=/tmp/xianggan VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 ## net.sf.picard.metrics.StringHeader # Started on: Thu Nov 04 17:47:28 GMT 2010 ## METRICS CLASS net.sf.picard.analysis.AlignmentSummaryMetrics CATEGORY TOTAL_READS PF_READS PCT_PF_READS PF_NOISE_READS PF_READS_ALIGNED PCT_PF_READS_ALIGNED PF_HQ_ALIGNED_READS PF_HQ_ALIGNED_BASES PF_HQ_ALIGNED_Q20_BASES PF_HQ_MEDIAN_MISMATCHES PF_HQ_ERROR_RATE MEAN_READ_LENGTH READS_ALIGNED_IN_PAIRS PCT_READS_ALIGNED_IN_PAIRS BAD_CYCLES STRAND_BALANCE PCT_CHIMERAS PCT_ADAPTER FIRST_OF_PAIR 95838612 95838612 1 0 93573495 0.976365 67104880 2750436053 2564727506 0 0.011044 40.840735 91656299 0.979511 0 0.497274 0.009149 0.000085 SECOND_OF_PAIR 95838612 95838612 1 0 92485426 0.965012 66508022 2722883563 2476962635 0 0.013788 40.840735 91656326 0.991035 0 0.502809 0.009161 0.000043 PAIR 191677224 191677224 1 0 186058921 0.970689 133612902 5473319616 5041690141 0 0.012409 40.840735 183312625 0.98524 0 0.500025 0.009155 0.000064 UNPAIRED 31446396 31446396 1 0 30511536 0.970271 22777873 1156903058 1123231471 0 0.009557 51 0 0 0 0.472582 0 0.00001