/home/xianggan/bin/exoner/tophat --num-threads 4 --library-type fr-firststrand --mate-inner-dist 38 --mate-std-dev 40 --max-multihits 10 --coverage-search --microexon-search -o Sf_2/ -G /data/www/arabcall/TAIR10_GFF3_genes_transposons.gff /data/arabidopsis/xiang/tair10_um Sf_2//Run32_lane8_Sf_2_seedling.fastq.flt_1.fq
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/gtf_juncs /data/www/arabcall/TAIR10_GFF3_genes_transposons.gff
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/prep_reads --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir Sf_2// --max-multihits 10 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --sam-header Sf_2//tmp/stub_header.sam --max-insertion-length 3 --max-deletion-length 3 --inner-dist-mean 38 --inner-dist-std-dev 40 --gtf-annotations /data/www/arabcall/TAIR10_GFF3_genes_transposons.gff --library-type fr-firststrand --fastq Sf_2//Run32_lane8_Sf_2_seedling.fastq.flt_1.fq
bowtie -q --un Sf_2//tmp/left_kept_reads_missing.fq --max /dev/null -v 2 -p 4 -k 10 -m 10 /data/arabidopsis/xiang/tair10_um Sf_2//tmp/left_kept_reads.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq Sf_2//tmp/left_kept_reads.fq - > Sf_2//tmp/file8qdRLn
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/long_spanning_reads --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir Sf_2// --max-multihits 10 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --sam-header Sf_2//tmp/stub_header.sam --max-insertion-length 3 --max-deletion-length 3 --inner-dist-mean 38 --inner-dist-std-dev 40 --gtf-annotations /data/www/arabcall/TAIR10_GFF3_genes_transposons.gff --library-type fr-firststrand /data/arabidopsis/xiang/tair10_um.fa Sf_2//tmp/left_kept_reads.fq /dev/null /dev/null /dev/null Sf_2//tmp/left_kept_reads.bwtout  > Sf_2//tmp/fileOU228s
bowtie -q --un Sf_2//tmp/left_kept_reads_seg1_missing.fq --max /dev/null -v 2 -p 4 -k 10 -m 10 /data/arabidopsis/xiang/tair10_um Sf_2//tmp/left_kept_reads_seg1.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq Sf_2//tmp/left_kept_reads_seg1.fq - > Sf_2//tmp/fileK3sASg
bowtie -q --un Sf_2//tmp/left_kept_reads_seg2_missing.fq --max /dev/null -v 2 -p 4 -k 10 -m 10 /data/arabidopsis/xiang/tair10_um Sf_2//tmp/left_kept_reads_seg2.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq Sf_2//tmp/left_kept_reads_seg2.fq - > Sf_2//tmp/fileCy9Y9A
bowtie -q --un Sf_2//tmp/left_kept_reads_seg3_missing.fq --max /dev/null -v 2 -p 4 -k 10 -m 10 /data/arabidopsis/xiang/tair10_um Sf_2//tmp/left_kept_reads_seg3.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq Sf_2//tmp/left_kept_reads_seg3.fq - > Sf_2//tmp/fileomrSSJ
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/segment_juncs --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir Sf_2// --max-multihits 10 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --sam-header Sf_2//tmp/stub_header.sam --max-insertion-length 3 --max-deletion-length 3 --inner-dist-mean 38 --inner-dist-std-dev 40 --gtf-annotations /data/www/arabcall/TAIR10_GFF3_genes_transposons.gff --no-closure-search --library-type fr-firststrand --ium-reads Sf_2//tmp/left_kept_reads_seg1_missing.fq,Sf_2//tmp/left_kept_reads_seg2_missing.fq,Sf_2//tmp/left_kept_reads_seg3_missing.fq /data/arabidopsis/xiang/tair10_um.fa Sf_2//tmp/segment.juncs Sf_2//tmp/segment.insertions Sf_2//tmp/segment.deletions Sf_2//tmp/left_kept_reads.fq Sf_2//tmp/left_kept_reads.bwtout Sf_2//tmp/left_kept_reads_seg1.bwtout,Sf_2//tmp/left_kept_reads_seg2.bwtout,Sf_2//tmp/left_kept_reads_seg3.bwtout
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/juncs_db 3 25 Sf_2//tmp/TAIR10_GFF3_genes_transposons.juncs,Sf_2//tmp/segment.juncs /dev/null /dev/null /data/arabidopsis/xiang/tair10_um.fa
bowtie-build Sf_2//tmp/segment_juncs.fa Sf_2//tmp/segment_juncs
bowtie -q -v 2 -p 4 -k 10 -m 10 Sf_2//tmp/segment_juncs Sf_2//tmp/left_kept_reads_seg1.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq Sf_2//tmp/left_kept_reads_seg1.fq - > Sf_2//tmp/fileLkfVXk
bowtie -q -v 2 -p 4 -k 10 -m 10 Sf_2//tmp/segment_juncs Sf_2//tmp/left_kept_reads_seg2.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq Sf_2//tmp/left_kept_reads_seg2.fq - > Sf_2//tmp/file72Tbh0
bowtie -q -v 2 -p 4 -k 10 -m 10 Sf_2//tmp/segment_juncs Sf_2//tmp/left_kept_reads_seg3.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq Sf_2//tmp/left_kept_reads_seg3.fq - > Sf_2//tmp/filePjsCGC
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/long_spanning_reads --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir Sf_2// --max-multihits 10 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --sam-header Sf_2//tmp/stub_header.sam --max-insertion-length 3 --max-deletion-length 3 --inner-dist-mean 38 --inner-dist-std-dev 40 --gtf-annotations /data/www/arabcall/TAIR10_GFF3_genes_transposons.gff --no-closure-search --library-type fr-firststrand /data/arabidopsis/xiang/tair10_um.fa Sf_2//tmp/left_kept_reads.fq Sf_2//tmp/TAIR10_GFF3_genes_transposons.juncs,Sf_2//tmp/segment.juncs /dev/null /dev/null Sf_2//tmp/left_kept_reads_seg1.bwtout,Sf_2//tmp/left_kept_reads_seg2.bwtout,Sf_2//tmp/left_kept_reads_seg3.bwtout Sf_2//tmp/left_kept_reads_seg1_to_spliced.bwtout,Sf_2//tmp/left_kept_reads_seg2_to_spliced.bwtout,Sf_2//tmp/left_kept_reads_seg3_to_spliced.bwtout > Sf_2//tmp/left_kept_reads.fq.candidate_hits.sam
sort -k 1,1n --temporary-directory=Sf_2//tmp/ Sf_2//tmp/fileOU228s Sf_2//tmp/left_kept_reads.fq.candidate_hits.sam > Sf_2//tmp/filefuc8jD
sort -k 1,1n --temporary-directory=Sf_2//tmp/ Sf_2//tmp/fileOU228s Sf_2//tmp/left_kept_reads.fq.candidate_hits.sam
mv Sf_2//tmp/filefuc8jD Sf_2//tmp/left_kept_reads.fq.candidate_hits.sam
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/tophat_reports --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir Sf_2// --max-multihits 10 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --sam-header Sf_2//tmp/stub_header.sam --max-insertion-length 3 --max-deletion-length 3 --inner-dist-mean 38 --inner-dist-std-dev 40 --gtf-annotations /data/www/arabcall/TAIR10_GFF3_genes_transposons.gff --no-closure-search --library-type fr-firststrand Sf_2//junctions.bed Sf_2//insertions.bed Sf_2//deletions.bed Sf_2//tmp/accepted_hits.sam Sf_2//tmp/left_kept_reads.fq.candidate_hits.sam Sf_2//tmp/left_kept_reads.fq
samtools view -S -b Sf_2//tmp/accepted_hits.sam > Sf_2//tmp/file4e8lc0
samtools sort Sf_2//tmp/file4e8lc0 Sf_2//accepted_hits