/home/xianggan/bin/exoner/tophat --num-threads 4 --mate-inner-dist 38 --mate-std-dev 40 --max-multihits 10 --coverage-search --microexon-search -o ./can_0 can_0.novel ./can_0/acc18_unmap.flt_1.fq
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/prep_reads --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir ./can_0/ --max-multihits 10 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --sam-header ./can_0/tmp/stub_header.sam --max-insertion-length 3 --max-deletion-length 3 --inner-dist-mean 38 --inner-dist-std-dev 40 --fastq ./can_0/acc18_unmap.flt_1.fq
bowtie -q --un ./can_0/tmp/left_kept_reads_missing.fq --max /dev/null -v 2 -p 4 -k 10 -m 10 can_0.novel ./can_0/tmp/left_kept_reads.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq ./can_0/tmp/left_kept_reads.fq - > ./can_0/tmp/file3wAI5n
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/long_spanning_reads --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir ./can_0/ --max-multihits 10 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --sam-header ./can_0/tmp/stub_header.sam --max-insertion-length 3 --max-deletion-length 3 --inner-dist-mean 38 --inner-dist-std-dev 40 can_0.novel.fa ./can_0/tmp/left_kept_reads.fq /dev/null /dev/null /dev/null ./can_0/tmp/left_kept_reads.bwtout  > ./can_0/tmp/fileSxCaIN
bowtie -q --un ./can_0/tmp/left_kept_reads_seg1_missing.fq --max /dev/null -v 2 -p 4 -k 10 -m 10 can_0.novel ./can_0/tmp/left_kept_reads_seg1.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq ./can_0/tmp/left_kept_reads_seg1.fq - > ./can_0/tmp/fileJI2JgI
bowtie -q --un ./can_0/tmp/left_kept_reads_seg2_missing.fq --max /dev/null -v 2 -p 4 -k 10 -m 10 can_0.novel ./can_0/tmp/left_kept_reads_seg2.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq ./can_0/tmp/left_kept_reads_seg2.fq - > ./can_0/tmp/fileAniXFO
bowtie -q --un ./can_0/tmp/left_kept_reads_seg3_missing.fq --max /dev/null -v 2 -p 4 -k 10 -m 10 can_0.novel ./can_0/tmp/left_kept_reads_seg3.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq ./can_0/tmp/left_kept_reads_seg3.fq - > ./can_0/tmp/fileb7XyLO
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/segment_juncs --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir ./can_0/ --max-multihits 10 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --sam-header ./can_0/tmp/stub_header.sam --max-insertion-length 3 --max-deletion-length 3 --inner-dist-mean 38 --inner-dist-std-dev 40 --no-closure-search --ium-reads ./can_0/tmp/left_kept_reads_seg1_missing.fq,./can_0/tmp/left_kept_reads_seg2_missing.fq,./can_0/tmp/left_kept_reads_seg3_missing.fq can_0.novel.fa ./can_0/tmp/segment.juncs ./can_0/tmp/segment.insertions ./can_0/tmp/segment.deletions ./can_0/tmp/left_kept_reads.fq ./can_0/tmp/left_kept_reads.bwtout ./can_0/tmp/left_kept_reads_seg1.bwtout,./can_0/tmp/left_kept_reads_seg2.bwtout,./can_0/tmp/left_kept_reads_seg3.bwtout
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/juncs_db 3 25 ./can_0/tmp/segment.juncs /dev/null /dev/null can_0.novel.fa
bowtie-build ./can_0/tmp/segment_juncs.fa ./can_0/tmp/segment_juncs
bowtie -q -v 2 -p 4 -k 10 -m 10 ./can_0/tmp/segment_juncs ./can_0/tmp/left_kept_reads_seg1.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq ./can_0/tmp/left_kept_reads_seg1.fq - > ./can_0/tmp/file2oSENE
bowtie -q -v 2 -p 4 -k 10 -m 10 ./can_0/tmp/segment_juncs ./can_0/tmp/left_kept_reads_seg2.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq ./can_0/tmp/left_kept_reads_seg2.fq - > ./can_0/tmp/file4vs1Gk
bowtie -q -v 2 -p 4 -k 10 -m 10 ./can_0/tmp/segment_juncs ./can_0/tmp/left_kept_reads_seg3.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq ./can_0/tmp/left_kept_reads_seg3.fq - > ./can_0/tmp/fileuh6i9G
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/long_spanning_reads --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir ./can_0/ --max-multihits 10 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --sam-header ./can_0/tmp/stub_header.sam --max-insertion-length 3 --max-deletion-length 3 --inner-dist-mean 38 --inner-dist-std-dev 40 --no-closure-search can_0.novel.fa ./can_0/tmp/left_kept_reads.fq ./can_0/tmp/segment.juncs /dev/null /dev/null ./can_0/tmp/left_kept_reads_seg1.bwtout,./can_0/tmp/left_kept_reads_seg2.bwtout,./can_0/tmp/left_kept_reads_seg3.bwtout ./can_0/tmp/left_kept_reads_seg1_to_spliced.bwtout,./can_0/tmp/left_kept_reads_seg2_to_spliced.bwtout,./can_0/tmp/left_kept_reads_seg3_to_spliced.bwtout > ./can_0/tmp/left_kept_reads.fq.candidate_hits.sam
sort -k 1,1n --temporary-directory=./can_0/tmp/ ./can_0/tmp/fileSxCaIN ./can_0/tmp/left_kept_reads.fq.candidate_hits.sam > ./can_0/tmp/fileMB48hX
sort -k 1,1n --temporary-directory=./can_0/tmp/ ./can_0/tmp/fileSxCaIN ./can_0/tmp/left_kept_reads.fq.candidate_hits.sam
mv ./can_0/tmp/fileMB48hX ./can_0/tmp/left_kept_reads.fq.candidate_hits.sam
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/tophat_reports --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir ./can_0/ --max-multihits 10 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --sam-header ./can_0/tmp/stub_header.sam --max-insertion-length 3 --max-deletion-length 3 --inner-dist-mean 38 --inner-dist-std-dev 40 --no-closure-search ./can_0/junctions.bed ./can_0/insertions.bed ./can_0/deletions.bed ./can_0/tmp/accepted_hits.sam ./can_0/tmp/left_kept_reads.fq.candidate_hits.sam ./can_0/tmp/left_kept_reads.fq
samtools view -S -b ./can_0/tmp/accepted_hits.sam > ./can_0/tmp/fileNpcsoY
samtools sort ./can_0/tmp/fileNpcsoY ./can_0/accepted_hits