/home/xianggan/bin/exoner/tophat --num-threads 4 --mate-inner-dist 38 --mate-std-dev 40 --max-multihits 10 --coverage-search --microexon-search -o ./kn_0.col kn_0.novel ./kn_0.col/col0Tokn_0.flt_1.fq
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/prep_reads --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir ./kn_0.col/ --max-multihits 10 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --sam-header ./kn_0.col/tmp/stub_header.sam --max-insertion-length 3 --max-deletion-length 3 --inner-dist-mean 38 --inner-dist-std-dev 40 --fastq ./kn_0.col/col0Tokn_0.flt_1.fq
bowtie -q --un ./kn_0.col/tmp/left_kept_reads_missing.fq --max /dev/null -v 2 -p 4 -k 10 -m 10 kn_0.novel ./kn_0.col/tmp/left_kept_reads.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq ./kn_0.col/tmp/left_kept_reads.fq - > ./kn_0.col/tmp/fileUmyrOh
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/long_spanning_reads --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir ./kn_0.col/ --max-multihits 10 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --sam-header ./kn_0.col/tmp/stub_header.sam --max-insertion-length 3 --max-deletion-length 3 --inner-dist-mean 38 --inner-dist-std-dev 40 kn_0.novel.fa ./kn_0.col/tmp/left_kept_reads.fq /dev/null /dev/null /dev/null ./kn_0.col/tmp/left_kept_reads.bwtout  > ./kn_0.col/tmp/fileLWGxtF
bowtie -q --un ./kn_0.col/tmp/left_kept_reads_seg1_missing.fq --max /dev/null -v 2 -p 4 -k 10 -m 10 kn_0.novel ./kn_0.col/tmp/left_kept_reads_seg1.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq ./kn_0.col/tmp/left_kept_reads_seg1.fq - > ./kn_0.col/tmp/file2x7VKT
bowtie -q --un ./kn_0.col/tmp/left_kept_reads_seg2_missing.fq --max /dev/null -v 2 -p 4 -k 10 -m 10 kn_0.novel ./kn_0.col/tmp/left_kept_reads_seg2.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq ./kn_0.col/tmp/left_kept_reads_seg2.fq - > ./kn_0.col/tmp/file1IYAeQ
bowtie -q --un ./kn_0.col/tmp/left_kept_reads_seg3_missing.fq --max /dev/null -v 2 -p 4 -k 10 -m 10 kn_0.novel ./kn_0.col/tmp/left_kept_reads_seg3.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq ./kn_0.col/tmp/left_kept_reads_seg3.fq - > ./kn_0.col/tmp/fileGtpe9D
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/segment_juncs --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir ./kn_0.col/ --max-multihits 10 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --sam-header ./kn_0.col/tmp/stub_header.sam --max-insertion-length 3 --max-deletion-length 3 --inner-dist-mean 38 --inner-dist-std-dev 40 --no-closure-search --ium-reads ./kn_0.col/tmp/left_kept_reads_seg1_missing.fq,./kn_0.col/tmp/left_kept_reads_seg2_missing.fq,./kn_0.col/tmp/left_kept_reads_seg3_missing.fq kn_0.novel.fa ./kn_0.col/tmp/segment.juncs ./kn_0.col/tmp/segment.insertions ./kn_0.col/tmp/segment.deletions ./kn_0.col/tmp/left_kept_reads.fq ./kn_0.col/tmp/left_kept_reads.bwtout ./kn_0.col/tmp/left_kept_reads_seg1.bwtout,./kn_0.col/tmp/left_kept_reads_seg2.bwtout,./kn_0.col/tmp/left_kept_reads_seg3.bwtout
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/juncs_db 3 25 ./kn_0.col/tmp/segment.juncs /dev/null /dev/null kn_0.novel.fa
bowtie-build ./kn_0.col/tmp/segment_juncs.fa ./kn_0.col/tmp/segment_juncs
bowtie -q -v 2 -p 4 -k 10 -m 10 ./kn_0.col/tmp/segment_juncs ./kn_0.col/tmp/left_kept_reads_seg1.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq ./kn_0.col/tmp/left_kept_reads_seg1.fq - > ./kn_0.col/tmp/file78Dm0g
bowtie -q -v 2 -p 4 -k 10 -m 10 ./kn_0.col/tmp/segment_juncs ./kn_0.col/tmp/left_kept_reads_seg2.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq ./kn_0.col/tmp/left_kept_reads_seg2.fq - > ./kn_0.col/tmp/fileMZeTmZ
bowtie -q -v 2 -p 4 -k 10 -m 10 ./kn_0.col/tmp/segment_juncs ./kn_0.col/tmp/left_kept_reads_seg3.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq ./kn_0.col/tmp/left_kept_reads_seg3.fq - > ./kn_0.col/tmp/fileK7JGrM
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/long_spanning_reads --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir ./kn_0.col/ --max-multihits 10 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --sam-header ./kn_0.col/tmp/stub_header.sam --max-insertion-length 3 --max-deletion-length 3 --inner-dist-mean 38 --inner-dist-std-dev 40 --no-closure-search kn_0.novel.fa ./kn_0.col/tmp/left_kept_reads.fq ./kn_0.col/tmp/segment.juncs /dev/null /dev/null ./kn_0.col/tmp/left_kept_reads_seg1.bwtout,./kn_0.col/tmp/left_kept_reads_seg2.bwtout,./kn_0.col/tmp/left_kept_reads_seg3.bwtout ./kn_0.col/tmp/left_kept_reads_seg1_to_spliced.bwtout,./kn_0.col/tmp/left_kept_reads_seg2_to_spliced.bwtout,./kn_0.col/tmp/left_kept_reads_seg3_to_spliced.bwtout > ./kn_0.col/tmp/left_kept_reads.fq.candidate_hits.sam
sort -k 1,1n --temporary-directory=./kn_0.col/tmp/ ./kn_0.col/tmp/fileLWGxtF ./kn_0.col/tmp/left_kept_reads.fq.candidate_hits.sam > ./kn_0.col/tmp/filetDLcGL
sort -k 1,1n --temporary-directory=./kn_0.col/tmp/ ./kn_0.col/tmp/fileLWGxtF ./kn_0.col/tmp/left_kept_reads.fq.candidate_hits.sam
mv ./kn_0.col/tmp/filetDLcGL ./kn_0.col/tmp/left_kept_reads.fq.candidate_hits.sam
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/tophat_reports --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir ./kn_0.col/ --max-multihits 10 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --sam-header ./kn_0.col/tmp/stub_header.sam --max-insertion-length 3 --max-deletion-length 3 --inner-dist-mean 38 --inner-dist-std-dev 40 --no-closure-search ./kn_0.col/junctions.bed ./kn_0.col/insertions.bed ./kn_0.col/deletions.bed ./kn_0.col/tmp/accepted_hits.sam ./kn_0.col/tmp/left_kept_reads.fq.candidate_hits.sam ./kn_0.col/tmp/left_kept_reads.fq
samtools view -S -b ./kn_0.col/tmp/accepted_hits.sam > ./kn_0.col/tmp/fileGGiayn
samtools sort ./kn_0.col/tmp/fileGGiayn ./kn_0.col/accepted_hits