segment_juncs v1.2.0 (1752) --------------------------- Loading reference sequences... Loading CUFF.1271_From_rsch_4_4034850_0...done Loading CUFF.1337_From_sf_2_2662232_0...done Loading CUFF.1285_From_rsch_4_4085063_0...done Loading CUFF.1565_From_zu_0_3075813_0...done Loading CUFF.1275_From_po_0_3332479_0...done Loading CUFF.1649_From_zu_0_3082201_0...done Loading CUFF.1293_From_rsch_4_4107621_0...done Loading CUFF.1163_From_no_0_2951521_0...done Loading CUFF.1465_From_ws_0_3190963_0...done Loading CUFF.1055_From_ler_0_4004770_0...done Loading CUFF.1335_From_sf_2_2524453_0...done Loading CUFF.907_From_can_0_3122480_0...done Loading CUFF.1481_From_ws_0_3349444_1...done Loading CUFF.1165_From_no_0_2959683_0...done Loading CUFF.1459_From_ws_0_3182508_0...done Loading CUFF.869_From_bur_0_1873494_0...done Loading CUFF.1463_From_ws_0_3190679_0...done Loading CUFF.921_From_can_0_3233581_0...done Loading CUFF.1367_From_tsu_0_3135135_0...done Loading CUFF.1133_From_mt_0_4399784_0...done Loading CUFF.1617_From_zu_0_3278243_0...done Loading CUFF.1497_From_ws_0_3380934_0...done Loading CUFF.1315_From_rsch_4_4277566_0...done Loading CUFF.1613_From_zu_0_3259399_1...done Loading CUFF.1401_From_wil_2_3050523_0...done Loading CUFF.989_From_edi_0_2932438_0...done Loading CUFF.1173_From_no_0_3113578_1...done Loading CUFF.1495_From_ws_0_3371988_0...done Loading CUFF.1611_From_zu_0_3255503_0...done Loading CUFF.1187_From_oy_0_3098144_0...done Loading CUFF.1011_From_edi_0_2918156_0...done Loading CUFF.1603_From_zu_0_3242889_0...done Loading CUFF.1531_From_ws_0_3340488_0...done Loading CUFF.1373_From_tsu_0_3208747_0...done Loading CUFF.865_From_bur_0_1967596_0...done Loading CUFF.1361_From_tsu_0_3175121_0...done Loading CUFF.1065_From_ler_0_4153595_0...done Loading CUFF.1439_From_wil_2_3089541_0...done Loading CUFF.1119_From_hi_0_2939638_0...done Loading CUFF.1117_From_hi_0_2939638_0...done Loading CUFF.1409_From_wil_2_3066229_0...done Loading CUFF.1595_From_zu_0_3238769_0...done Loading CUFF.1077_From_ler_0_4225073_0...done Loading CUFF.913_From_can_0_3217045_0...done Loading CUFF.985_From_edi_0_2921730_0...done Loading CUFF.1281_From_oy_0_3121110_0...done Loading CUFF.973_From_edi_0_2871668_0...done Loading CUFF.943_From_ct_1_3247037_0...done Loading CUFF.1069_From_hi_0_2916184_0...done Loading CUFF.1035_From_kn_0_3751597_0...done Loading CUFF.1543_From_ws_0_3373604_0...done Loading CUFF.983_From_ct_1_3241157_0...done Loading CUFF.1311_From_rsch_4_4275442_0...done Loading CUFF.909_From_can_0_3223655_0...done Loading CUFF.1479_From_ws_0_3339092_0...done Loading CUFF.1443_From_wil_2_3052273_0...done Loading CUFF.881_From_bur_0_1969548_0...done Loading CUFF.1637_From_zu_0_3277661_0...done Loading CUFF.1313_From_po_0_3573778_0...done Loading CUFF.1385_From_sf_2_2653966_0...done Loading CUFF.1169_From_no_0_3101262_0...done Loading CUFF.1419_From_sf_2_2701328_0...done Loading CUFF.1111_From_ler_0_4165287_0...done Loading CUFF.1029_From_kn_0_3802200_0...done Loading CUFF.1625_From_zu_0_3243571_0...done Loading CUFF.1407_From_wil_2_3063473_0...done Loading CUFF.919_From_can_0_3258779_0...done Loading CUFF.1383_From_tsu_0_3242833_1...done Loading CUFF.1375_From_tsu_0_3211741_0...done Loading CUFF.1347_From_sf_2_2682779_0...done Loading CUFF.1493_From_wil_2_3087183_0...done Loading CUFF.1557_From_wu_0_2824308_0...done Loading CUFF.879_From_bur_0_2021834_0...done Loading CUFF.1377_From_tsu_0_3215671_0...done Loading CUFF.1421_From_tsu_0_3215821_0...done Loading CUFF.915_From_can_0_3231205_0...done Loading CUFF.875_From_bur_0_1999718_0...done Loading CUFF.949_From_can_0_3218459_0...done Loading CUFF.1521_From_ws_0_3344070_0...done Loading CUFF.1405_From_wil_2_3052951_0...done Loading CUFF.1089_From_ler_0_4224097_0...done Loading CUFF.1159_From_no_0_3088052_0...done Loading CUFF.1363_From_sf_2_2671632_0...done Loading CUFF.1471_From_tsu_0_3212935_0...done Loading CUFF.1007_From_edi_0_2888586_0...done Loading CUFF.1455_From_wil_2_3068737_0...done Loading CUFF.937_From_can_0_3239881_0...done Loading CUFF.1607_From_zu_0_3255169_0...done Loading CUFF.971_From_edi_0_2891226_0...done Loading CUFF.1623_From_zu_0_3289907_1...done Loading CUFF.1411_From_rsch_4_4266550_0...done Loading CUFF.1177_From_no_0_3116640_0...done Loading CUFF.1451_From_wil_2_3077447_0...done Loading CUFF.1647_From_zu_0_3289907_1...done Loading CUFF.1487_From_ws_0_3347582_0...done Loading CUFF.1175_From_hi_0_2953648_0...done Loading CUFF.1437_From_wil_2_3088151_0...done Loading CUFF.1033_From_kn_0_3799876_0...done Loading CUFF.889_From_bur_0_2032354_0...done Loading CUFF.935_From_bur_0_2020452_0...done Loading CUFF.1215_From_oy_0_3162092_0...done Loading CUFF.1633_From_zu_0_3280213_0...done Loading CUFF.1289_From_po_0_3606391_0...done Loading CUFF.1501_From_ws_0_3365892_0...done Loading CUFF.1585_From_wu_0_2837848_0...done Loading CUFF.1053_From_kn_0_3819922_1...done Loading CUFF.1267_From_no_0_3128142_0...done Loading CUFF.1413_From_sf_2_2687786_0...done Loading CUFF.1181_From_no_0_3120392_0...done Loading CUFF.941_From_bur_0_2025286_0...done Loading CUFF.1369_From_sf_2_2700286_0...done Loading CUFF.997_From_edi_0_2934714_0...done Loading CUFF.1537_From_ws_0_3382500_0...done Loading CUFF.1211_From_po_0_3269389_0...done Loading CUFF.1519_From_wu_0_2597064_0...done Loading CUFF.1507_From_wu_0_2587993_0...done Loading CUFF.1209_From_po_0_3295404_0...done Loading CUFF.1523_From_wu_0_2638225_0...done Loading CUFF.981_From_hi_0_2690171_0...done Loading CUFF.1027_From_kn_0_3558357_0...done Loading CUFF.1093_From_mt_0_4130072_0...done Loading CUFF.1219_From_po_0_3319326_0...done Loading CUFF.1547_From_wu_0_2685428_0...done Loading CUFF.1353_From_tsu_0_3002533_0...done Loading CUFF.1569_From_zu_0_3035650_0...done Loading CUFF.1141_From_mt_0_4449890_0...done Loading CUFF.1529_From_wu_0_2655159_0...done Loading CUFF.1575_From_wu_0_2831218_0...done Loading CUFF.1013_From_kn_0_3532396_0...done Loading CUFF.1095_From_mt_0_4131964_0...done Loading CUFF.1081_From_mt_0_4099484_0...done Loading CUFF.1515_From_wu_0_2648785_0...done Loading CUFF.1153_From_no_0_2923846_0...done Loading CUFF.1307_From_rsch_4_4277566_0...done Loading CUFF.1213_From_po_0_3316390_0...done Loading CUFF.1003_From_hi_0_2790034_0...done Loading CUFF.1631_From_zu_0_3277661_1...done Loading CUFF.1207_From_po_0_3308932_0...done Loading CUFF.1397_From_tsu_0_3228017_0...done Loading CUFF.1008_From_kn_0_3525938_0...done Loading CUFF.1017_From_kn_0_3566907_0...done Loading CUFF.1573_From_zu_0_3065917_0...done Loading CUFF.1099_From_mt_0_4177596_0...done Loading CUFF.1253_From_po_0_3387411_0...done Loading CUFF.1453_From_ws_0_3185408_0...done Loading CUFF.1599_From_zu_0_3200689_0...done Loading CUFF.1229_From_po_0_3339041_0...done Loading CUFF.1061_From_ler_0_4016532_0...done Loading CUFF.901_From_can_0_3094988_0...done Loading CUFF.1629_From_zu_0_3287917_0...done Loading CUFF.1139_From_ler_0_4211779_1...done Loading CUFF.1287_From_rsch_4_4099439_0...done Loading CUFF.1101_From_mt_0_4188374_0...done Loading CUFF.953_From_edi_0_2759591_0...done Loading CUFF.1597_From_zu_0_3181459_0...done Loading CUFF.1233_From_po_0_3365309_0...done Loading CUFF.991_From_edi_0_2891968_0...done Loading CUFF.993_From_hi_0_2787534_0...done Loading CUFF.1049_From_ler_0_4002120_0...done Loading CUFF.1539_From_wu_0_2702886_0...done Loading CUFF.1113_From_mt_0_4206392_0...done Loading CUFF.1431_From_ws_0_3170660_0...done Loading CUFF.1527_From_wu_0_2675967_0...done Loading CUFF.1259_From_po_0_3402348_0...done Loading CUFF.1379_From_sf_2_2656790_0...done Loading CUFF.955_From_edi_0_2766531_0...done Loading CUFF.1491_From_ws_0_3373966_0...done Loading CUFF.1121_From_mt_0_4226690_0...done Loading CUFF.1489_From_ws_0_3372620_0...done Loading CUFF.1087_From_ler_0_4211779_0...done Loading CUFF.1559_From_wu_0_2824162_0...done Loading CUFF.967_From_edi_0_2887084_2...done Loading CUFF.1645_From_wu_0_2816600_0...done Loading CUFF.1473_From_ws_0_3293243_0...done Loading CUFF.1399_From_wil_2_2978735_0...done Loading CUFF.969_From_edi_0_2888616_0...done Loading CUFF.1305_From_rsch_4_4263688_0...done Loading CUFF.911_From_can_0_3230893_0...done Loading CUFF.1639_From_zu_0_3291057_0...done Loading CUFF.1167_From_no_0_3096552_0...done Loading CUFF.1475_From_ws_0_3313321_0...done Loading CUFF.1197_From_oy_0_3103794_0...done Loading CUFF.1359_From_tsu_0_3148935_0...done Loading CUFF.1223_From_po_0_3340213_0...done Loading CUFF.1329_From_sf_2_2642906_0...done Loading CUFF.1641_From_zu_0_3291057_0...done Loading CUFF.1423_From_wil_2_3072219_0...done Loading CUFF.1325_From_sf_2_2626132_0...done Loading CUFF.1417_From_wil_2_3078633_1...done Loading CUFF.1583_From_wu_0_2829124_0...done Loading CUFF.1309_From_rsch_4_4307314_0...done Loading CUFF.1605_From_zu_0_3257369_0...done Loading CUFF.1345_From_sf_2_2689374_0...done Loading CUFF.1619_From_zu_0_3280025_0...done Loading CUFF.975_From_edi_0_2902016_0...done Loading CUFF.1545_From_wu_0_2770362_0...done Loading CUFF.1643_From_wu_0_2816600_0...done Loading CUFF.1609_From_zu_0_3260873_0...done Loading CUFF.1189_From_oy_0_3115434_0...done Loading CUFF.1467_From_ws_0_3309675_0...done Loading CUFF.1553_From_wu_0_2806776_0...done Loading CUFF.1265_From_po_0_3577252_0...done Loading CUFF.1073_From_ler_0_4209589_1...done Loading CUFF.1635_From_zu_0_3263539_0...done Loading CUFF.1579_From_wu_0_2802868_0...done Loading CUFF.1513_From_ws_0_3381290_0...done Loading CUFF.1415_From_wil_2_3082811_1...done Loading CUFF.1393_From_tsu_0_3228555_0...done Loading CUFF.1333_From_sf_2_2666154_1...done Loading CUFF.1015_From_hi_0_2966608_0...done Loading CUFF.951_From_ct_1_3280175_0...done Loading CUFF.871_From_bur_0_2012810_0...done Loading CUFF.1371_From_tsu_0_3211333_0...done Loading CUFF.1279_From_po_0_3599571_0...done Loading CUFF.1577_From_wu_0_2832848_0...done Loading CUFF.1561_From_wu_0_2827904_0...done Loading CUFF.1041_From_kn_0_3824150_0...done Loading CUFF.1627_From_zu_0_3262887_0...done Loading CUFF.1381_From_tsu_0_3240885_0...done Loading CUFF.1277_From_po_0_3599571_0...done Loading CUFF.1447_From_wil_2_3091415_1...done Loading left segment hits... Microaligned 387 segments done. Found 38 potential split-segment junctions Found 4 potential small deletions Found 5 potential small insertions Indexing extensions in ./Mt_0_R2.TOnovel/tmp/left_kept_reads_seg1_missing.fq Indexing extensions in ./Mt_0_R2.TOnovel/tmp/left_kept_reads_seg2_missing.fq Indexing extensions in ./Mt_0_R2.TOnovel/tmp/left_kept_reads_seg3_missing.fq Total extensions: 19107470 Total extensions: 19107470 Total extensions: 19107470 Looking for junctions by island end pairings Adding hits from segment file 0 to coverage map Adding hits from segment file 1 to coverage map Adding hits from segment file 2 to coverage map Map covers 10361 bases Map covers 10199 bases in sufficiently long segments Map contains 161 good islands 4952 are left looking bases 3625 are right looking bases Collecting potential splice sites in islands reporting synthetic splice junctions... Examining donor-acceptor pairings in CUFF.951_From_ct_1_3280175_0 Examining donor-acceptor pairings in CUFF.1471_From_tsu_0_3212935_0 Examining donor-acceptor pairings in CUFF.1371_From_tsu_0_3211333_0 Examining donor-acceptor pairings in CUFF.1279_From_po_0_3599571_0 Examining donor-acceptor pairings in CUFF.1133_From_mt_0_4399784_0 Examining donor-acceptor pairings in CUFF.1253_From_po_0_3387411_0 Examining donor-acceptor pairings in CUFF.1035_From_kn_0_3751597_0 Examining donor-acceptor pairings in CUFF.1219_From_po_0_3319326_0 Examining donor-acceptor pairings in CUFF.1399_From_wil_2_2978735_0 Examining donor-acceptor pairings in CUFF.1531_From_ws_0_3340488_0 Examining donor-acceptor pairings in CUFF.1577_From_wu_0_2832848_0 Examining donor-acceptor pairings in CUFF.1611_From_zu_0_3255503_0 Examining donor-acceptor pairings in CUFF.989_From_edi_0_2932438_0 Examining donor-acceptor pairings in CUFF.1631_From_zu_0_3277661_1 Examining donor-acceptor pairings in CUFF.1637_From_zu_0_3277661_0 Examining donor-acceptor pairings in CUFF.1117_From_hi_0_2939638_0 Examining donor-acceptor pairings in CUFF.1215_From_oy_0_3162092_0 Examining donor-acceptor pairings in CUFF.911_From_can_0_3230893_0 Examining donor-acceptor pairings in CUFF.1177_From_no_0_3116640_0 Examining donor-acceptor pairings in CUFF.1437_From_wil_2_3088151_0 Examining donor-acceptor pairings in CUFF.1101_From_mt_0_4188374_0 Examining donor-acceptor pairings in CUFF.1099_From_mt_0_4177596_0 Examining donor-acceptor pairings in CUFF.1573_From_zu_0_3065917_0 Examining donor-acceptor pairings in CUFF.1197_From_oy_0_3103794_0 Examining donor-acceptor pairings in CUFF.1289_From_po_0_3606391_0 Examining donor-acceptor pairings in CUFF.1277_From_po_0_3599571_0 Examining donor-acceptor pairings in CUFF.1259_From_po_0_3402348_0 Examining donor-acceptor pairings in CUFF.1017_From_kn_0_3566907_0 Examining donor-acceptor pairings in CUFF.1359_From_tsu_0_3148935_0 Examining donor-acceptor pairings in CUFF.919_From_can_0_3258779_0 Examining donor-acceptor pairings in CUFF.1447_From_wil_2_3091415_1 Examining donor-acceptor pairings in CUFF.1641_From_zu_0_3291057_0 Examining donor-acceptor pairings in CUFF.1169_From_no_0_3101262_0 Examining donor-acceptor pairings in CUFF.1003_From_hi_0_2790034_0 Examining donor-acceptor pairings in CUFF.1579_From_wu_0_2802868_0 Examining donor-acceptor pairings in CUFF.1383_From_tsu_0_3242833_1 Examining donor-acceptor pairings in CUFF.1335_From_sf_2_2524453_0 Examining donor-acceptor pairings in CUFF.1547_From_wu_0_2685428_0 Examining donor-acceptor pairings in CUFF.871_From_bur_0_2012810_0 Examining donor-acceptor pairings in CUFF.935_From_bur_0_2020452_0 Examining donor-acceptor pairings in CUFF.1453_From_ws_0_3185408_0 Examining donor-acceptor pairings in CUFF.1163_From_no_0_2951521_0 Examining donor-acceptor pairings in CUFF.1635_From_zu_0_3263539_0 Examining donor-acceptor pairings in CUFF.1049_From_ler_0_4002120_0 Examining donor-acceptor pairings in CUFF.1633_From_zu_0_3280213_0 Examining donor-acceptor pairings in CUFF.1495_From_ws_0_3371988_0 Examining donor-acceptor pairings in CUFF.1529_From_wu_0_2655159_0 Examining donor-acceptor pairings in CUFF.1491_From_ws_0_3373966_0 Examining donor-acceptor pairings in CUFF.1213_From_po_0_3316390_0 Examining donor-acceptor pairings in CUFF.967_From_edi_0_2887084_2 Examining donor-acceptor pairings in CUFF.1119_From_hi_0_2939638_0 Examining donor-acceptor pairings in CUFF.1649_From_zu_0_3082201_0 Examining donor-acceptor pairings in CUFF.1053_From_kn_0_3819922_1 Examining donor-acceptor pairings in CUFF.1431_From_ws_0_3170660_0 Examining donor-acceptor pairings in CUFF.1095_From_mt_0_4131964_0 Examining donor-acceptor pairings in CUFF.1421_From_tsu_0_3215821_0 Examining donor-acceptor pairings in CUFF.1481_From_ws_0_3349444_1 Examining donor-acceptor pairings in CUFF.1475_From_ws_0_3313321_0 Examining donor-acceptor pairings in CUFF.1267_From_no_0_3128142_0 Examining donor-acceptor pairings in CUFF.1139_From_ler_0_4211779_1 Examining donor-acceptor pairings in CUFF.1087_From_ler_0_4211779_0 Examining donor-acceptor pairings in CUFF.1141_From_mt_0_4449890_0 Found 0 potential island-end pairing junctions done Aligning 387 microexon segments in 101 windows window 100 Checked 774 segments against 101 windows for microexon junctions Found 1 potential microexon junctions -- microexon -- CUFF.1421_From_tsu_0_3215821_0 511 620 - -- done -- -- seg -- CUFF.1577_From_wu_0_2832848_0 341 433 + CUFF.1577_From_wu_0_2832848_0 349 433 + CUFF.1577_From_wu_0_2832848_0 363 433 - CUFF.1577_From_wu_0_2832848_0 366 433 + CUFF.1579_From_wu_0_2802868_0 190 578 - CUFF.1579_From_wu_0_2802868_0 190 593 + CUFF.1579_From_wu_0_2802868_0 197 602 - CUFF.1579_From_wu_0_2802868_0 204 578 - CUFF.1579_From_wu_0_2802868_0 204 593 + CUFF.1579_From_wu_0_2802868_0 206 602 + CUFF.1579_From_wu_0_2802868_0 213 578 - CUFF.1579_From_wu_0_2802868_0 213 593 + CUFF.1579_From_wu_0_2802868_0 218 578 - CUFF.1579_From_wu_0_2802868_0 218 593 + CUFF.1579_From_wu_0_2802868_0 221 602 + CUFF.1579_From_wu_0_2802868_0 224 593 + CUFF.1579_From_wu_0_2802868_0 225 602 - CUFF.1579_From_wu_0_2802868_0 227 602 + CUFF.1577_From_wu_0_2832848_0 341 445 + CUFF.1577_From_wu_0_2832848_0 349 445 + CUFF.1577_From_wu_0_2832848_0 363 445 - CUFF.1577_From_wu_0_2832848_0 366 445 + CUFF.1579_From_wu_0_2802868_0 190 581 - CUFF.1579_From_wu_0_2802868_0 190 596 + CUFF.1579_From_wu_0_2802868_0 204 581 - CUFF.1579_From_wu_0_2802868_0 204 596 + CUFF.1579_From_wu_0_2802868_0 213 581 - CUFF.1579_From_wu_0_2802868_0 213 596 + CUFF.1579_From_wu_0_2802868_0 218 581 - CUFF.1579_From_wu_0_2802868_0 218 596 + CUFF.1579_From_wu_0_2802868_0 224 596 + CUFF.1577_From_wu_0_2832848_0 341 454 + CUFF.1577_From_wu_0_2832848_0 349 454 + CUFF.1577_From_wu_0_2832848_0 363 454 - CUFF.1577_From_wu_0_2832848_0 366 454 + CUFF.1579_From_wu_0_2802868_0 190 604 + CUFF.1579_From_wu_0_2802868_0 204 604 + CUFF.1579_From_wu_0_2802868_0 213 604 + -- done -- -- cov -- -- done -- -- buf -- -- done -- Reporting potential splice junctions...done Reported 39 total possible splices Reporting potential deletions... Reporting potential insertions...