segment_juncs v1.2.0 (1752) --------------------------- Loading reference sequences... Loading CUFF.1271_From_rsch_4_4034850_0...done Loading CUFF.1337_From_sf_2_2662232_0...done Loading CUFF.1285_From_rsch_4_4085063_0...done Loading CUFF.1565_From_zu_0_3075813_0...done Loading CUFF.1275_From_po_0_3332479_0...done Loading CUFF.1649_From_zu_0_3082201_0...done Loading CUFF.1293_From_rsch_4_4107621_0...done Loading CUFF.1163_From_no_0_2951521_0...done Loading CUFF.1465_From_ws_0_3190963_0...done Loading CUFF.1055_From_ler_0_4004770_0...done Loading CUFF.1335_From_sf_2_2524453_0...done Loading CUFF.907_From_can_0_3122480_0...done Loading CUFF.1481_From_ws_0_3349444_1...done Loading CUFF.1165_From_no_0_2959683_0...done Loading CUFF.1459_From_ws_0_3182508_0...done Loading CUFF.869_From_bur_0_1873494_0...done Loading CUFF.1463_From_ws_0_3190679_0...done Loading CUFF.921_From_can_0_3233581_0...done Loading CUFF.1367_From_tsu_0_3135135_0...done Loading CUFF.1133_From_mt_0_4399784_0...done Loading CUFF.1617_From_zu_0_3278243_0...done Loading CUFF.1497_From_ws_0_3380934_0...done Loading CUFF.1315_From_rsch_4_4277566_0...done Loading CUFF.1613_From_zu_0_3259399_1...done Loading CUFF.1401_From_wil_2_3050523_0...done Loading CUFF.989_From_edi_0_2932438_0...done Loading CUFF.1173_From_no_0_3113578_1...done Loading CUFF.1495_From_ws_0_3371988_0...done Loading CUFF.1611_From_zu_0_3255503_0...done Loading CUFF.1187_From_oy_0_3098144_0...done Loading CUFF.1011_From_edi_0_2918156_0...done Loading CUFF.1603_From_zu_0_3242889_0...done Loading CUFF.1531_From_ws_0_3340488_0...done Loading CUFF.1373_From_tsu_0_3208747_0...done Loading CUFF.865_From_bur_0_1967596_0...done Loading CUFF.1361_From_tsu_0_3175121_0...done Loading CUFF.1065_From_ler_0_4153595_0...done Loading CUFF.1439_From_wil_2_3089541_0...done Loading CUFF.1119_From_hi_0_2939638_0...done Loading CUFF.1117_From_hi_0_2939638_0...done Loading CUFF.1409_From_wil_2_3066229_0...done Loading CUFF.1595_From_zu_0_3238769_0...done Loading CUFF.1077_From_ler_0_4225073_0...done Loading CUFF.913_From_can_0_3217045_0...done Loading CUFF.985_From_edi_0_2921730_0...done Loading CUFF.1281_From_oy_0_3121110_0...done Loading CUFF.973_From_edi_0_2871668_0...done Loading CUFF.943_From_ct_1_3247037_0...done Loading CUFF.1069_From_hi_0_2916184_0...done Loading CUFF.1035_From_kn_0_3751597_0...done Loading CUFF.1543_From_ws_0_3373604_0...done Loading CUFF.983_From_ct_1_3241157_0...done Loading CUFF.1311_From_rsch_4_4275442_0...done Loading CUFF.909_From_can_0_3223655_0...done Loading CUFF.1479_From_ws_0_3339092_0...done Loading CUFF.1443_From_wil_2_3052273_0...done Loading CUFF.881_From_bur_0_1969548_0...done Loading CUFF.1637_From_zu_0_3277661_0...done Loading CUFF.1313_From_po_0_3573778_0...done Loading CUFF.1385_From_sf_2_2653966_0...done Loading CUFF.1169_From_no_0_3101262_0...done Loading CUFF.1419_From_sf_2_2701328_0...done Loading CUFF.1111_From_ler_0_4165287_0...done Loading CUFF.1029_From_kn_0_3802200_0...done Loading CUFF.1625_From_zu_0_3243571_0...done Loading CUFF.1407_From_wil_2_3063473_0...done Loading CUFF.919_From_can_0_3258779_0...done Loading CUFF.1383_From_tsu_0_3242833_1...done Loading CUFF.1375_From_tsu_0_3211741_0...done Loading CUFF.1347_From_sf_2_2682779_0...done Loading CUFF.1493_From_wil_2_3087183_0...done Loading CUFF.1557_From_wu_0_2824308_0...done Loading CUFF.879_From_bur_0_2021834_0...done Loading CUFF.1377_From_tsu_0_3215671_0...done Loading CUFF.1421_From_tsu_0_3215821_0...done Loading CUFF.915_From_can_0_3231205_0...done Loading CUFF.875_From_bur_0_1999718_0...done Loading CUFF.949_From_can_0_3218459_0...done Loading CUFF.1521_From_ws_0_3344070_0...done Loading CUFF.1405_From_wil_2_3052951_0...done Loading CUFF.1089_From_ler_0_4224097_0...done Loading CUFF.1159_From_no_0_3088052_0...done Loading CUFF.1363_From_sf_2_2671632_0...done Loading CUFF.1471_From_tsu_0_3212935_0...done Loading CUFF.1007_From_edi_0_2888586_0...done Loading CUFF.1455_From_wil_2_3068737_0...done Loading CUFF.937_From_can_0_3239881_0...done Loading CUFF.1607_From_zu_0_3255169_0...done Loading CUFF.971_From_edi_0_2891226_0...done Loading CUFF.1623_From_zu_0_3289907_1...done Loading CUFF.1411_From_rsch_4_4266550_0...done Loading CUFF.1177_From_no_0_3116640_0...done Loading CUFF.1451_From_wil_2_3077447_0...done Loading CUFF.1647_From_zu_0_3289907_1...done Loading CUFF.1487_From_ws_0_3347582_0...done Loading CUFF.1175_From_hi_0_2953648_0...done Loading CUFF.1437_From_wil_2_3088151_0...done Loading CUFF.1033_From_kn_0_3799876_0...done Loading CUFF.889_From_bur_0_2032354_0...done Loading CUFF.935_From_bur_0_2020452_0...done Loading CUFF.1215_From_oy_0_3162092_0...done Loading CUFF.1633_From_zu_0_3280213_0...done Loading CUFF.1289_From_po_0_3606391_0...done Loading CUFF.1501_From_ws_0_3365892_0...done Loading CUFF.1585_From_wu_0_2837848_0...done Loading CUFF.1053_From_kn_0_3819922_1...done Loading CUFF.1267_From_no_0_3128142_0...done Loading CUFF.1413_From_sf_2_2687786_0...done Loading CUFF.1181_From_no_0_3120392_0...done Loading CUFF.941_From_bur_0_2025286_0...done Loading CUFF.1369_From_sf_2_2700286_0...done Loading CUFF.997_From_edi_0_2934714_0...done Loading CUFF.1537_From_ws_0_3382500_0...done Loading CUFF.1211_From_po_0_3269389_0...done Loading CUFF.1519_From_wu_0_2597064_0...done Loading CUFF.1507_From_wu_0_2587993_0...done Loading CUFF.1209_From_po_0_3295404_0...done Loading CUFF.1523_From_wu_0_2638225_0...done Loading CUFF.981_From_hi_0_2690171_0...done Loading CUFF.1027_From_kn_0_3558357_0...done Loading CUFF.1093_From_mt_0_4130072_0...done Loading CUFF.1219_From_po_0_3319326_0...done Loading CUFF.1547_From_wu_0_2685428_0...done Loading CUFF.1353_From_tsu_0_3002533_0...done Loading CUFF.1569_From_zu_0_3035650_0...done Loading CUFF.1141_From_mt_0_4449890_0...done Loading CUFF.1529_From_wu_0_2655159_0...done Loading CUFF.1575_From_wu_0_2831218_0...done Loading CUFF.1013_From_kn_0_3532396_0...done Loading CUFF.1095_From_mt_0_4131964_0...done Loading CUFF.1081_From_mt_0_4099484_0...done Loading CUFF.1515_From_wu_0_2648785_0...done Loading CUFF.1153_From_no_0_2923846_0...done Loading CUFF.1307_From_rsch_4_4277566_0...done Loading CUFF.1213_From_po_0_3316390_0...done Loading CUFF.1003_From_hi_0_2790034_0...done Loading CUFF.1631_From_zu_0_3277661_1...done Loading CUFF.1207_From_po_0_3308932_0...done Loading CUFF.1397_From_tsu_0_3228017_0...done Loading CUFF.1008_From_kn_0_3525938_0...done Loading CUFF.1017_From_kn_0_3566907_0...done Loading CUFF.1573_From_zu_0_3065917_0...done Loading CUFF.1099_From_mt_0_4177596_0...done Loading CUFF.1253_From_po_0_3387411_0...done Loading CUFF.1453_From_ws_0_3185408_0...done Loading CUFF.1599_From_zu_0_3200689_0...done Loading CUFF.1229_From_po_0_3339041_0...done Loading CUFF.1061_From_ler_0_4016532_0...done Loading CUFF.901_From_can_0_3094988_0...done Loading CUFF.1629_From_zu_0_3287917_0...done Loading CUFF.1139_From_ler_0_4211779_1...done Loading CUFF.1287_From_rsch_4_4099439_0...done Loading CUFF.1101_From_mt_0_4188374_0...done Loading CUFF.953_From_edi_0_2759591_0...done Loading CUFF.1597_From_zu_0_3181459_0...done Loading CUFF.1233_From_po_0_3365309_0...done Loading CUFF.991_From_edi_0_2891968_0...done Loading CUFF.993_From_hi_0_2787534_0...done Loading CUFF.1049_From_ler_0_4002120_0...done Loading CUFF.1539_From_wu_0_2702886_0...done Loading CUFF.1113_From_mt_0_4206392_0...done Loading CUFF.1431_From_ws_0_3170660_0...done Loading CUFF.1527_From_wu_0_2675967_0...done Loading CUFF.1259_From_po_0_3402348_0...done Loading CUFF.1379_From_sf_2_2656790_0...done Loading CUFF.955_From_edi_0_2766531_0...done Loading CUFF.1491_From_ws_0_3373966_0...done Loading CUFF.1121_From_mt_0_4226690_0...done Loading CUFF.1489_From_ws_0_3372620_0...done Loading CUFF.1087_From_ler_0_4211779_0...done Loading CUFF.1559_From_wu_0_2824162_0...done Loading CUFF.967_From_edi_0_2887084_2...done Loading CUFF.1645_From_wu_0_2816600_0...done Loading CUFF.1473_From_ws_0_3293243_0...done Loading CUFF.1399_From_wil_2_2978735_0...done Loading CUFF.969_From_edi_0_2888616_0...done Loading CUFF.1305_From_rsch_4_4263688_0...done Loading CUFF.911_From_can_0_3230893_0...done Loading CUFF.1639_From_zu_0_3291057_0...done Loading CUFF.1167_From_no_0_3096552_0...done Loading CUFF.1475_From_ws_0_3313321_0...done Loading CUFF.1197_From_oy_0_3103794_0...done Loading CUFF.1359_From_tsu_0_3148935_0...done Loading CUFF.1223_From_po_0_3340213_0...done Loading CUFF.1329_From_sf_2_2642906_0...done Loading CUFF.1641_From_zu_0_3291057_0...done Loading CUFF.1423_From_wil_2_3072219_0...done Loading CUFF.1325_From_sf_2_2626132_0...done Loading CUFF.1417_From_wil_2_3078633_1...done Loading CUFF.1583_From_wu_0_2829124_0...done Loading CUFF.1309_From_rsch_4_4307314_0...done Loading CUFF.1605_From_zu_0_3257369_0...done Loading CUFF.1345_From_sf_2_2689374_0...done Loading CUFF.1619_From_zu_0_3280025_0...done Loading CUFF.975_From_edi_0_2902016_0...done Loading CUFF.1545_From_wu_0_2770362_0...done Loading CUFF.1643_From_wu_0_2816600_0...done Loading CUFF.1609_From_zu_0_3260873_0...done Loading CUFF.1189_From_oy_0_3115434_0...done Loading CUFF.1467_From_ws_0_3309675_0...done Loading CUFF.1553_From_wu_0_2806776_0...done Loading CUFF.1265_From_po_0_3577252_0...done Loading CUFF.1073_From_ler_0_4209589_1...done Loading CUFF.1635_From_zu_0_3263539_0...done Loading CUFF.1579_From_wu_0_2802868_0...done Loading CUFF.1513_From_ws_0_3381290_0...done Loading CUFF.1415_From_wil_2_3082811_1...done Loading CUFF.1393_From_tsu_0_3228555_0...done Loading CUFF.1333_From_sf_2_2666154_1...done Loading CUFF.1015_From_hi_0_2966608_0...done Loading CUFF.951_From_ct_1_3280175_0...done Loading CUFF.871_From_bur_0_2012810_0...done Loading CUFF.1371_From_tsu_0_3211333_0...done Loading CUFF.1279_From_po_0_3599571_0...done Loading CUFF.1577_From_wu_0_2832848_0...done Loading CUFF.1561_From_wu_0_2827904_0...done Loading CUFF.1041_From_kn_0_3824150_0...done Loading CUFF.1627_From_zu_0_3262887_0...done Loading CUFF.1381_From_tsu_0_3240885_0...done Loading CUFF.1277_From_po_0_3599571_0...done Loading CUFF.1447_From_wil_2_3091415_1...done Loading left segment hits... Microaligned 137 segments done. Found 71 potential split-segment junctions Found 3 potential small deletions Found 2 potential small insertions Indexing extensions in ./Ws_0_R1.TOnovel/tmp/left_kept_reads_seg1_missing.fq Indexing extensions in ./Ws_0_R1.TOnovel/tmp/left_kept_reads_seg2_missing.fq Indexing extensions in ./Ws_0_R1.TOnovel/tmp/left_kept_reads_seg3_missing.fq Total extensions: 19456902 Total extensions: 19456902 Total extensions: 19456902 Looking for junctions by island end pairings Adding hits from segment file 0 to coverage map Adding hits from segment file 1 to coverage map Adding hits from segment file 2 to coverage map Map covers 9604 bases Map covers 9455 bases in sufficiently long segments Map contains 149 good islands 4800 are left looking bases 3314 are right looking bases Collecting potential splice sites in islands reporting synthetic splice junctions... Examining donor-acceptor pairings in CUFF.951_From_ct_1_3280175_0 Examining donor-acceptor pairings in CUFF.1605_From_zu_0_3257369_0 Examining donor-acceptor pairings in CUFF.1467_From_ws_0_3309675_0 Examining donor-acceptor pairings in CUFF.1279_From_po_0_3599571_0 Examining donor-acceptor pairings in CUFF.1173_From_no_0_3113578_1 Examining donor-acceptor pairings in CUFF.1035_From_kn_0_3751597_0 Examining donor-acceptor pairings in CUFF.1459_From_ws_0_3182508_0 Examining donor-acceptor pairings in CUFF.1219_From_po_0_3319326_0 Examining donor-acceptor pairings in CUFF.1629_From_zu_0_3287917_0 Examining donor-acceptor pairings in CUFF.1487_From_ws_0_3347582_0 Examining donor-acceptor pairings in CUFF.1275_From_po_0_3332479_0 Examining donor-acceptor pairings in CUFF.1015_From_hi_0_2966608_0 Examining donor-acceptor pairings in CUFF.1609_From_zu_0_3260873_0 Examining donor-acceptor pairings in CUFF.1521_From_ws_0_3344070_0 Examining donor-acceptor pairings in CUFF.1513_From_ws_0_3381290_0 Examining donor-acceptor pairings in CUFF.1489_From_ws_0_3372620_0 Examining donor-acceptor pairings in CUFF.1643_From_wu_0_2816600_0 Examining donor-acceptor pairings in CUFF.1265_From_po_0_3577252_0 Examining donor-acceptor pairings in CUFF.1117_From_hi_0_2939638_0 Examining donor-acceptor pairings in CUFF.911_From_can_0_3230893_0 Examining donor-acceptor pairings in CUFF.1437_From_wil_2_3088151_0 Examining donor-acceptor pairings in CUFF.869_From_bur_0_1873494_0 Examining donor-acceptor pairings in CUFF.1101_From_mt_0_4188374_0 Examining donor-acceptor pairings in CUFF.1223_From_po_0_3340213_0 Examining donor-acceptor pairings in CUFF.1451_From_wil_2_3077447_0 Examining donor-acceptor pairings in CUFF.1229_From_po_0_3339041_0 Examining donor-acceptor pairings in CUFF.1277_From_po_0_3599571_0 Examining donor-acceptor pairings in CUFF.1575_From_wu_0_2831218_0 Examining donor-acceptor pairings in CUFF.955_From_edi_0_2766531_0 Examining donor-acceptor pairings in CUFF.1259_From_po_0_3402348_0 Examining donor-acceptor pairings in CUFF.1017_From_kn_0_3566907_0 Examining donor-acceptor pairings in CUFF.1361_From_tsu_0_3175121_0 Examining donor-acceptor pairings in CUFF.1167_From_no_0_3096552_0 Examining donor-acceptor pairings in CUFF.1639_From_zu_0_3291057_0 Examining donor-acceptor pairings in CUFF.1061_From_ler_0_4016532_0 Examining donor-acceptor pairings in CUFF.1447_From_wil_2_3091415_1 Examining donor-acceptor pairings in CUFF.1641_From_zu_0_3291057_0 Examining donor-acceptor pairings in CUFF.1003_From_hi_0_2790034_0 Examining donor-acceptor pairings in CUFF.1547_From_wu_0_2685428_0 Examining donor-acceptor pairings in CUFF.907_From_can_0_3122480_0 Examining donor-acceptor pairings in CUFF.1479_From_ws_0_3339092_0 Examining donor-acceptor pairings in CUFF.1559_From_wu_0_2824162_0 Examining donor-acceptor pairings in CUFF.1311_From_rsch_4_4275442_0 Examining donor-acceptor pairings in CUFF.1553_From_wu_0_2806776_0 Examining donor-acceptor pairings in CUFF.1309_From_rsch_4_4307314_0 Examining donor-acceptor pairings in CUFF.973_From_edi_0_2871668_0 Examining donor-acceptor pairings in CUFF.1049_From_ler_0_4002120_0 Examining donor-acceptor pairings in CUFF.1529_From_wu_0_2655159_0 Examining donor-acceptor pairings in CUFF.943_From_ct_1_3247037_0 Examining donor-acceptor pairings in CUFF.969_From_edi_0_2888616_0 Examining donor-acceptor pairings in CUFF.1111_From_ler_0_4165287_0 Examining donor-acceptor pairings in CUFF.1543_From_ws_0_3373604_0 Examining donor-acceptor pairings in CUFF.1473_From_ws_0_3293243_0 Examining donor-acceptor pairings in CUFF.1649_From_zu_0_3082201_0 Examining donor-acceptor pairings in CUFF.1423_From_wil_2_3072219_0 Examining donor-acceptor pairings in CUFF.1431_From_ws_0_3170660_0 Examining donor-acceptor pairings in CUFF.1481_From_ws_0_3349444_1 Examining donor-acceptor pairings in CUFF.1475_From_ws_0_3313321_0 Examining donor-acceptor pairings in CUFF.1267_From_no_0_3128142_0 Examining donor-acceptor pairings in CUFF.1645_From_wu_0_2816600_0 Examining donor-acceptor pairings in CUFF.1141_From_mt_0_4449890_0 Found 2 potential island-end pairing junctions done Aligning 137 microexon segments in 88 windows Checked 274 segments against 88 windows for microexon junctions Found 0 potential microexon junctions -- microexon -- -- done -- -- seg -- CUFF.1437_From_wil_2_3088151_0 455 519 - CUFF.1437_From_wil_2_3088151_0 455 521 - CUFF.1437_From_wil_2_3088151_0 459 519 + CUFF.1437_From_wil_2_3088151_0 462 519 - CUFF.1437_From_wil_2_3088151_0 462 521 - CUFF.1437_From_wil_2_3088151_0 464 519 - CUFF.1437_From_wil_2_3088151_0 464 521 - CUFF.1437_From_wil_2_3088151_0 466 519 - CUFF.1437_From_wil_2_3088151_0 466 521 - CUFF.1111_From_ler_0_4165287_0 535 642 + CUFF.1111_From_ler_0_4165287_0 537 645 - CUFF.1111_From_ler_0_4165287_0 537 654 + CUFF.1111_From_ler_0_4165287_0 539 645 - CUFF.1111_From_ler_0_4165287_0 539 654 + CUFF.1111_From_ler_0_4165287_0 541 642 + CUFF.1111_From_ler_0_4165287_0 543 645 - CUFF.1111_From_ler_0_4165287_0 543 654 + CUFF.1111_From_ler_0_4165287_0 549 642 - CUFF.1111_From_ler_0_4165287_0 552 642 - CUFF.1543_From_ws_0_3373604_0 303 389 - CUFF.1543_From_ws_0_3373604_0 306 389 + CUFF.1543_From_ws_0_3373604_0 309 389 + CUFF.1543_From_ws_0_3373604_0 313 389 - CUFF.1543_From_ws_0_3373604_0 317 389 + CUFF.1543_From_ws_0_3373604_0 325 392 + CUFF.1543_From_ws_0_3373604_0 328 389 + CUFF.1543_From_ws_0_3373604_0 330 389 + CUFF.1543_From_ws_0_3373604_0 333 389 + CUFF.1543_From_ws_0_3373604_0 337 397 + CUFF.1437_From_wil_2_3088151_0 455 525 - CUFF.1437_From_wil_2_3088151_0 459 525 + CUFF.1437_From_wil_2_3088151_0 462 525 - CUFF.1437_From_wil_2_3088151_0 464 525 - CUFF.1437_From_wil_2_3088151_0 466 525 - CUFF.1111_From_ler_0_4165287_0 537 647 - CUFF.1111_From_ler_0_4165287_0 537 657 + CUFF.1111_From_ler_0_4165287_0 539 647 - CUFF.1111_From_ler_0_4165287_0 539 657 + CUFF.1111_From_ler_0_4165287_0 543 647 - CUFF.1111_From_ler_0_4165287_0 543 657 + CUFF.1543_From_ws_0_3373604_0 303 397 - CUFF.1543_From_ws_0_3373604_0 306 397 + CUFF.1543_From_ws_0_3373604_0 309 397 + CUFF.1543_From_ws_0_3373604_0 313 397 - CUFF.1543_From_ws_0_3373604_0 317 397 + CUFF.1543_From_ws_0_3373604_0 325 395 + CUFF.1543_From_ws_0_3373604_0 328 397 + CUFF.1543_From_ws_0_3373604_0 330 397 + CUFF.1543_From_ws_0_3373604_0 333 397 + CUFF.1543_From_ws_0_3373604_0 337 400 + CUFF.1111_From_ler_0_4165287_0 537 660 + CUFF.1111_From_ler_0_4165287_0 537 664 - CUFF.1111_From_ler_0_4165287_0 539 660 + CUFF.1111_From_ler_0_4165287_0 539 664 - CUFF.1111_From_ler_0_4165287_0 543 660 + CUFF.1111_From_ler_0_4165287_0 543 664 - CUFF.1543_From_ws_0_3373604_0 303 400 - CUFF.1543_From_ws_0_3373604_0 306 400 + CUFF.1543_From_ws_0_3373604_0 309 400 + CUFF.1543_From_ws_0_3373604_0 313 400 - CUFF.1543_From_ws_0_3373604_0 317 400 + CUFF.1543_From_ws_0_3373604_0 325 403 + CUFF.1543_From_ws_0_3373604_0 328 400 + CUFF.1543_From_ws_0_3373604_0 330 400 + CUFF.1543_From_ws_0_3373604_0 333 400 + CUFF.1543_From_ws_0_3373604_0 337 407 + CUFF.1543_From_ws_0_3373604_0 313 407 - CUFF.1543_From_ws_0_3373604_0 317 407 + CUFF.1543_From_ws_0_3373604_0 328 407 + CUFF.1543_From_ws_0_3373604_0 330 407 + CUFF.1543_From_ws_0_3373604_0 333 407 + -- done -- -- cov -- CUFF.1423_From_wil_2_3072219_0 47 981 + CUFF.1475_From_ws_0_3313321_0 26 393 - -- done -- -- buf -- -- done -- Reporting potential splice junctions...done Reported 73 total possible splices Reporting potential deletions... Reporting potential insertions...