/home/xianggan/bin/exoner/tophat --num-threads 4 --library-type fr-firststrand --mate-inner-dist 38 --mate-std-dev 40 --max-multihits 10 --coverage-search --microexon-search -o Can_0/ -G novel221.gtf novel221.contig Can_0//Run33_lane1_Can_0_seedling.fastq.unmap_1.flt_1.fq
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/gtf_juncs novel221.gtf
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/prep_reads --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir Can_0// --max-multihits 10 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --sam-header Can_0//tmp/stub_header.sam --max-insertion-length 3 --max-deletion-length 3 --inner-dist-mean 38 --inner-dist-std-dev 40 --gtf-annotations novel221.gtf --library-type fr-firststrand --fastq Can_0//Run33_lane1_Can_0_seedling.fastq.unmap_1.flt_1.fq
bowtie -q --un Can_0//tmp/left_kept_reads_missing.fq --max /dev/null -v 2 -p 4 -k 10 -m 10 novel221.contig Can_0//tmp/left_kept_reads.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq Can_0//tmp/left_kept_reads.fq - > Can_0//tmp/file1oTDp4
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/long_spanning_reads --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir Can_0// --max-multihits 10 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --sam-header Can_0//tmp/stub_header.sam --max-insertion-length 3 --max-deletion-length 3 --inner-dist-mean 38 --inner-dist-std-dev 40 --gtf-annotations novel221.gtf --library-type fr-firststrand novel221.contig.fa Can_0//tmp/left_kept_reads.fq /dev/null /dev/null /dev/null Can_0//tmp/left_kept_reads.bwtout  > Can_0//tmp/file1t3vy6
bowtie -q --un Can_0//tmp/left_kept_reads_seg1_missing.fq --max /dev/null -v 2 -p 4 -k 10 -m 10 novel221.contig Can_0//tmp/left_kept_reads_seg1.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq Can_0//tmp/left_kept_reads_seg1.fq - > Can_0//tmp/fileRNzUj0
bowtie -q --un Can_0//tmp/left_kept_reads_seg2_missing.fq --max /dev/null -v 2 -p 4 -k 10 -m 10 novel221.contig Can_0//tmp/left_kept_reads_seg2.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq Can_0//tmp/left_kept_reads_seg2.fq - > Can_0//tmp/filedXL8mr
bowtie -q --un Can_0//tmp/left_kept_reads_seg3_missing.fq --max /dev/null -v 2 -p 4 -k 10 -m 10 novel221.contig Can_0//tmp/left_kept_reads_seg3.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq Can_0//tmp/left_kept_reads_seg3.fq - > Can_0//tmp/file5vZ7iv
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/segment_juncs --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir Can_0// --max-multihits 10 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --sam-header Can_0//tmp/stub_header.sam --max-insertion-length 3 --max-deletion-length 3 --inner-dist-mean 38 --inner-dist-std-dev 40 --gtf-annotations novel221.gtf --no-closure-search --library-type fr-firststrand --ium-reads Can_0//tmp/left_kept_reads_seg1_missing.fq,Can_0//tmp/left_kept_reads_seg2_missing.fq,Can_0//tmp/left_kept_reads_seg3_missing.fq novel221.contig.fa Can_0//tmp/segment.juncs Can_0//tmp/segment.insertions Can_0//tmp/segment.deletions Can_0//tmp/left_kept_reads.fq Can_0//tmp/left_kept_reads.bwtout Can_0//tmp/left_kept_reads_seg1.bwtout,Can_0//tmp/left_kept_reads_seg2.bwtout,Can_0//tmp/left_kept_reads_seg3.bwtout
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/juncs_db 3 25 Can_0//tmp/novel221.juncs,Can_0//tmp/segment.juncs /dev/null /dev/null novel221.contig.fa
bowtie-build Can_0//tmp/segment_juncs.fa Can_0//tmp/segment_juncs
bowtie -q -v 2 -p 4 -k 10 -m 10 Can_0//tmp/segment_juncs Can_0//tmp/left_kept_reads_seg1.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq Can_0//tmp/left_kept_reads_seg1.fq - > Can_0//tmp/fileeUj0cL
bowtie -q -v 2 -p 4 -k 10 -m 10 Can_0//tmp/segment_juncs Can_0//tmp/left_kept_reads_seg2.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq Can_0//tmp/left_kept_reads_seg2.fq - > Can_0//tmp/fileZz2KAi
bowtie -q -v 2 -p 4 -k 10 -m 10 Can_0//tmp/segment_juncs Can_0//tmp/left_kept_reads_seg3.fq | /Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/fix_map_ordering --fastq Can_0//tmp/left_kept_reads_seg3.fq - > Can_0//tmp/fileGZV4kU
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/long_spanning_reads --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir Can_0// --max-multihits 10 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --sam-header Can_0//tmp/stub_header.sam --max-insertion-length 3 --max-deletion-length 3 --inner-dist-mean 38 --inner-dist-std-dev 40 --gtf-annotations novel221.gtf --no-closure-search --library-type fr-firststrand novel221.contig.fa Can_0//tmp/left_kept_reads.fq Can_0//tmp/novel221.juncs,Can_0//tmp/segment.juncs /dev/null /dev/null Can_0//tmp/left_kept_reads_seg1.bwtout,Can_0//tmp/left_kept_reads_seg2.bwtout,Can_0//tmp/left_kept_reads_seg3.bwtout Can_0//tmp/left_kept_reads_seg1_to_spliced.bwtout,Can_0//tmp/left_kept_reads_seg2_to_spliced.bwtout,Can_0//tmp/left_kept_reads_seg3_to_spliced.bwtout > Can_0//tmp/left_kept_reads.fq.candidate_hits.sam
sort -k 1,1n --temporary-directory=Can_0//tmp/ Can_0//tmp/file1t3vy6 Can_0//tmp/left_kept_reads.fq.candidate_hits.sam > Can_0//tmp/fileKeiHPm
sort -k 1,1n --temporary-directory=Can_0//tmp/ Can_0//tmp/file1t3vy6 Can_0//tmp/left_kept_reads.fq.candidate_hits.sam
mv Can_0//tmp/fileKeiHPm Can_0//tmp/left_kept_reads.fq.candidate_hits.sam
/Net/fs1/home/xianggan/bin/tophat-1.2.0.Linux_x86_64/tophat_reports --min-anchor 8 --splice-mismatches 0 --min-report-intron 50 --max-report-intron 500000 --min-isoform-fraction 0.15 --output-dir Can_0// --max-multihits 10 --segment-length 25 --segment-mismatches 2 --min-closure-exon 100 --min-closure-intron 50 --max-closure-intron 5000 --min-coverage-intron 50 --max-coverage-intron 20000 --min-segment-intron 50 --max-segment-intron 500000 --sam-header Can_0//tmp/stub_header.sam --max-insertion-length 3 --max-deletion-length 3 --inner-dist-mean 38 --inner-dist-std-dev 40 --gtf-annotations novel221.gtf --no-closure-search --library-type fr-firststrand Can_0//junctions.bed Can_0//insertions.bed Can_0//deletions.bed Can_0//tmp/accepted_hits.sam Can_0//tmp/left_kept_reads.fq.candidate_hits.sam Can_0//tmp/left_kept_reads.fq
samtools view -S -b Can_0//tmp/accepted_hits.sam > Can_0//tmp/fileDFyyjw
samtools sort Can_0//tmp/fileDFyyjw Can_0//accepted_hits