This track displays regions bound by Sp1 and Sp3, in the following three cell lines, assayed by ChIP and microarray hybridization:
Cell Line Classification Isolated From HCT 116 colorectal carcinoma colon Jurkat, Clone E6-1 acute T cell leukemia T lymphocyte K-562 chronic myelogenous leukemia (CML) bone marrow
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Chromatin IP was performed as described in Trinklein et al. (2004). Amplified and labeled ChIP DNA was hybridized to oligo tiling arrays produced by NimbleGen, along with a total genomic reference sample. The data for each array were median subtracted (log 2 ratios) and normalized (divided by the standard deviation). The value given for each probe is the transformed mean ratio of ChIP DNA:Total DNA.
Three biological replicates and two technical replicates were performed. The Myers lab is currently testing the specificity and sensitivity using real-time PCR.
These data were generated in the Richard M. Myers lab at Stanford University (now at HudsonAlpha Institute for Biotechnology).
Trinklein, N.D., Chen, W.C., Kingston, R.E. and Myers, R.M. The role of heat shock transcription factor 1 in the genome-wide regulation of the mammalian heat shock response. Mol. Biol. Cell 15(3), 1254-61 (2004).