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| Denisova cave entrance in the Altai Mountains of Siberia, Russia where the bones were found from which DNA was sequenced (Copyright (C) 2010, Johannes Krause) |
The Denisova Sequence track shows high-coverage sequence reads from an archaic Denisovan individual mapped to the $organism genome reference assembly. The Denisova DNA was extracted from a phalanx bone excavated from Denisova Cave in the Altai Mountains in southern Siberia.
A novel single-stranded DNA library preparation method (Note 2, supplementary online materials of Meyer, 2012) was applied to DNA previously extracted from 40mg of bone (Reich, 2010). Using single-stranded DNA greatly increased the genomic coverage to 30X compared to an earlier 1.9X sequence (Reich, 2010). Sequence reads were aligned to $organism sequence $date (downloaded from the 1000 Genomes Project) using the Burrows-Wheeler Aligner.
Thanks to the Max Planck Institute for Evolutionary Anthropology for providing the BAM files used for this track.
Meyer M, Kircher M, Gansauge MT, Li H, Racimo F, Mallick S, Schraiber JG, Jay F, Prüfer K, de Filippo C et al. A High-Coverage Genome Sequence from an Archaic Denisovan Individual. Science. 2012 Aug 31. [Epub ahead of print] supplementary online materials, Note 2
Reich D, Green RE, Kircher M, Krause J, Patterson N, Durand EY, Viola B, Briggs AW, Stenzel U, Johnson PLF et al. Genetic history of an archaic hominin group from Denisova Cave in Siberia. Nature. 2010 Dec 23;468:1053-1060.