This track displays the probes from the Illumina WG-6 3.0 BeadChip. The WG-6 BeadChip contains probes for the following set of RNA transcripts:
Probe source | Number of probes | Number of unique probe sources |
RefSeq NM (well-established coding transcript) | 27,454 | 22,435 |
RefSeq XM (provisional coding transcript) | 7,870 | 7,518 |
RefSeq NR (well-established non-coding transcript) | 446 | 358 |
RefSeq XR (provisional non-coding transcript) | 196 | 190 |
UniGene ESTs | 12,837 | 12,837 |
TOTAL | 48,803 | 43,338 |
The track shows the location of the probes on the genome after the RNAs they correspond to were all aligned to the genome using BLAT. Alignment scores range from 0 to 1000, where 1000 is a perfect score. In the display, darker browns are for higher-scoring alignments.
Click on a probe track item to see detailed information about that probe ID. View the base-by-base alignment for that probe by clicking the "View Alignment" link on the details page.
The probe set was collected from the NCBI GEO (Gene Expression Omnibus), and the 43,338 RNA sequences were collected from Genbank using NCBI's EUtils interface to Entrez. These RNAs were aligned to the genome using BLAT, and 43,224 of them aligned well to 46,432 locations on the genome. The single best alignment was used, except in 1,789 cases where the RNA mapped equally well to two or more locations. The probes were then aligned to their respective RNAs using BLAT, and if a good alignment resulted, the probe was then mapped through to the genome using the combination of the probe-on-RNA and the RNA-on-genome alignments. Of the 48,803 original probes, 40,852 map well through this procedure to 44,163 locations on the genome.