The plot represents relative mononucleosome enrichment at each position in the genome (on a log of 2 scale). The coverage metric is given according to a formula [ (1+n)/N ] / [ (1+c)/C ] where n and c are the numbers of putative 147 base pair cores covering each base pair from nucleosome and control data, N and C are the total number of nucleosome and control reads obtained by SOLiD sequencing with 25 base pairs mapped to the reference genome.
The source data for this track can be fetched from our
ftp server
(nucleosomeAdjustedCoverage.wigAscii.gz; 450 Mb compressed,
1.6 Gb uncompressed).
This data is formatted into the two-column variableStep
wiggle format.
Valouev A, Ichikawa J, Tonthat T, Stuart J, Ranade S, Peckham H, Zeng K, Malek JA, Costa G, McKernan K, Sidow A, Fire A, Johnson SM. A high-resolution, nucleosome position map of C. elegans reveals a lack of universal sequence-dictated positioning. Genome Res. 2008 Jul;18(7):1051-63. Epub 2008 May 13.
Supported oligo ligation detection (SOLiD) sequencing technology from Applied Biosystems.