The plot represents local coverage by control micrococcal nuclease fragments from cleavage of naked C. elegans DNA. By analogy with the experimental nucleosome data, 147 base pair sense segments starting from each of the individual control reads has been used to assemble a control coverage plot that accounts for enzymatic and sequencing biases of sampling from the reference genome.
The plot represents local coverage by control micrococcal nuclease fragments from cleavage of naked C. elegans DNA. By analogy with the experimental nucleosome data, 147 base pair antisense segments starting from each of the individual control reads has been used to assemble a control coverage plot that accounts for enzymatic and sequencing biases of sampling from the reference genome.
Valouev A, Ichikawa J, Tonthat T, Stuart J, Ranade S, Peckham H, Zeng K, Malek JA, Costa G, McKernan K, Sidow A, Fire A, Johnson SM. A high-resolution, nucleosome position map of C. elegans reveals a lack of universal sequence-dictated positioning. Genome Res. 2008 Jul;18(7):1051-63. Epub 2008 May 13.
Supported oligo ligation detection (SOLiD) sequencing technology from Applied Biosystems.