This track shows the Zebrafish Zv7 (July 2007) assembly provided by The Wellcome Trust Sanger Institute. It contains scaffolds (supercontigs) totaling 1,440,582,308 bp. The assembly has been tied to the fingerprint contig (FPC) map (data freeze April 11, 2007), which provides a tiling path of sequenced clones. 1.02 Gb of sequence from 7,823 sequenced clones (7,139 finished and 684 unfinished) were used as a scaffold for the assembly. Gaps were filled with contigs from a whole genome shotgun (WGS) assembly of 5.5x coverage comprised of reads from a library created from a single Tuebingen doubled haploid zebrafish. Approximately 1.28 Gbp (89%) of the resulting integrated assembly were placed on chromosomes 1-25, including estimated gap sizes and 100 bp gaps inserted betweeen scaffolds. The complete sequence of the mitochondrion genome, which is shown as chrM in the Genome Browser, was obtained from GenBank.
The entire assembly of 5,036 scaffolds includes the 26 chromosomes and 5,010 unplaced scaffolds that fall into two groups:
All components within this track are of fragment type "W" (WGS contig) except for chrM, which is type "F" (Finished).
In dense mode, this track depicts the path through the draft and finished clones (aka the golden path) used to create the assembled sequence. Clone boundaries are distinguished by the use of alternating gold and brown coloration. Where gaps exist in the path, spaces are shown between the gold and brown blocks. If the relative order and orientation of the scaffolds between the two blocks is known, a line is drawn to bridge the blocks.
For detailed information on the methods used to produce this assembly, see the Wellcome Trust Sanger Institute Danio rerio Sequencing Project website.
The Zv7 Zebrafish assembly was produced by The Wellcome Trust Sanger Institute, in collaboration with the Max Planck Institute for Developmental Biology, the Netherlands Institute for Developmental Biology (Hubrecht Laboratory), and Yi Zhou, Anthony DiBiase and Leonard Zon from the Boston Children's Hospital.