/***************************************************************************** mergeMain.cpp (c) 2009 - Aaron Quinlan Hall Laboratory Department of Biochemistry and Molecular Genetics University of Virginia aaronquinlan@gmail.com Licenced under the GNU General Public License 2.0 license. ******************************************************************************/ #include "mergeBed.h" #include "version.h" using namespace std; // define our program name #define PROGRAM_NAME "bedtools merge" // define our parameter checking macro #define PARAMETER_CHECK(param, paramLen, actualLen) (strncmp(argv[i], param, min(actualLen, paramLen))== 0) && (actualLen == paramLen) // function declarations void merge_help(void); int merge_main(int argc, char* argv[]) { // our configuration variables bool showHelp = false; // input files string bedFile = "stdin"; int maxDistance = 0; string scoreOp = ""; // input arguments bool haveBed = true; bool numEntries = false; bool haveMaxDistance = false; bool forceStrand = false; bool reportNames = false; bool reportScores = false; for(int i = 1; i < argc; i++) { int parameterLength = (int)strlen(argv[i]); if((PARAMETER_CHECK("-h", 2, parameterLength)) || (PARAMETER_CHECK("--help", 5, parameterLength))) { showHelp = true; } } if(showHelp) merge_help(); // do some parsing (all of these parameters require 2 strings) for(int i = 1; i < argc; i++) { int parameterLength = (int)strlen(argv[i]); if(PARAMETER_CHECK("-i", 2, parameterLength)) { if ((i+1) < argc) { bedFile = argv[i + 1]; i++; } } else if(PARAMETER_CHECK("-n", 2, parameterLength)) { numEntries = true; } else if(PARAMETER_CHECK("-d", 2, parameterLength)) { if ((i+1) < argc) { haveMaxDistance = true; maxDistance = atoi(argv[i + 1]); i++; } } else if (PARAMETER_CHECK("-s", 2, parameterLength)) { forceStrand = true; } else if (PARAMETER_CHECK("-nms", 4, parameterLength)) { reportNames = true; } else if (PARAMETER_CHECK("-scores", 7, parameterLength)) { reportScores = true; if ((i+1) < argc) { scoreOp = argv[i + 1]; i++; } } else { cerr << endl << "*****ERROR: Unrecognized parameter: " << argv[i] << " *****" << endl << endl; showHelp = true; } } // make sure we have both input files if (!haveBed) { cerr << endl << "*****" << endl << "*****ERROR: Need -i BED file. " << endl << "*****" << endl; showHelp = true; } if ((reportScores == true) && (scoreOp != "sum") && (scoreOp != "max") && (scoreOp != "min") && (scoreOp != "mean") && (scoreOp != "mode") && (scoreOp != "median") && (scoreOp != "antimode") && (scoreOp != "collapse")) { cerr << endl << "*****" << endl << "*****ERROR: Invalid scoreOp selection \"" << scoreOp << endl << "\" *****" << endl; showHelp = true; } if (!showHelp) { BedMerge *bm = new BedMerge(bedFile, numEntries, maxDistance, forceStrand, reportNames, reportScores, scoreOp); delete bm; } else { merge_help(); } return 0; } void merge_help(void) { cerr << "\nTool: bedtools merge (aka mergeBed)" << endl; cerr << "Version: " << VERSION << "\n"; cerr << "Summary: Merges overlapping BED/GFF/VCF entries into a single interval." << endl << endl; cerr << "Usage: " << PROGRAM_NAME << " [OPTIONS] -i " << endl << endl; cerr << "Options: " << endl; cerr << "\t-s\t" << "Force strandedness. That is, only merge features" << endl; cerr << "\t\tthat are the same strand." << endl; cerr << "\t\t- By default, merging is done without respect to strand." << endl << endl; cerr << "\t-n\t" << "Report the number of BED entries that were merged." << endl; cerr << "\t\t- Note: \"1\" is reported if no merging occurred." << endl << endl; cerr << "\t-d\t" << "Maximum distance between features allowed for features" << endl; cerr << "\t\tto be merged." << endl; cerr << "\t\t- Def. 0. That is, overlapping & book-ended features are merged." << endl; cerr << "\t\t- (INTEGER)" << endl << endl; cerr << "\t-nms\t" << "Report the names of the merged features separated by semicolons." << endl << endl; cerr << "\t-scores\t" << "Report the scores of the merged features. Specify one of " << endl; cerr << "\t\tthe following options for reporting scores:" << endl; cerr << "\t\t sum, min, max," << endl; cerr << "\t\t mean, median, mode, antimode," << endl; cerr << "\t\t collapse (i.e., print a semicolon-separated list)," << endl; cerr << "\t\t- (INTEGER)" << endl << endl; cerr << "Notes: " << endl; cerr << "\t(1) All output, regardless of input type (e.g., GFF or VCF)" << endl; cerr << "\t will in BED format with zero-based starts" << endl << endl; cerr << "\t(2) The input file (-i) file must be sorted by chrom, then start." << endl << endl; // end the program here exit(1); }