/***************************************************************************** subtractMain.cpp (c) 2009 - Aaron Quinlan Hall Laboratory Department of Biochemistry and Molecular Genetics University of Virginia aaronquinlan@gmail.com Licenced under the GNU General Public License 2.0 license. ******************************************************************************/ #include "subtractBed.h" #include "version.h" using namespace std; // define our program name #define PROGRAM_NAME "bedtools subtract" // define our parameter checking macro #define PARAMETER_CHECK(param, paramLen, actualLen) \ (strncmp(argv[i], param, min(actualLen, paramLen))== 0) && \ (actualLen == paramLen) // function declarations void subtract_help(void); int subtract_main(int argc, char* argv[]) { // our configuration variables bool showHelp = false; // input files string bedAFile; string bedBFile; // input arguments float overlapFraction = 1E-9; bool haveBedA = false; bool haveBedB = false; bool haveFraction = false; bool sameStrand = false; bool diffStrand = false; bool removeAll = false; // check to see if we should print out some help if(argc <= 1) showHelp = true; for(int i = 1; i < argc; i++) { int parameterLength = (int)strlen(argv[i]); if((PARAMETER_CHECK("-h", 2, parameterLength)) || (PARAMETER_CHECK("--help", 5, parameterLength))) { showHelp = true; } } if(showHelp) subtract_help(); // do some parsing (all of these parameters require 2 strings) for(int i = 1; i < argc; i++) { int parameterLength = (int)strlen(argv[i]); if(PARAMETER_CHECK("-a", 2, parameterLength)) { if ((i+1) < argc) { haveBedA = true; bedAFile = argv[i + 1]; i++; } } else if(PARAMETER_CHECK("-b", 2, parameterLength)) { if ((i+1) < argc) { haveBedB = true; bedBFile = argv[i + 1]; i++; } } else if(PARAMETER_CHECK("-f", 2, parameterLength)) { if ((i+1) < argc) { haveFraction = true; overlapFraction = atof(argv[i + 1]); i++; } } else if (PARAMETER_CHECK("-s", 2, parameterLength)) { sameStrand = true; } else if (PARAMETER_CHECK("-S", 2, parameterLength)) { diffStrand = true; } else if (PARAMETER_CHECK("-A", 2, parameterLength)) { removeAll = true; } else { cerr << endl << "*****ERROR: Unrecognized parameter: " << argv[i] << " *****" << endl << endl; showHelp = true; } } // make sure we have both input files if (!haveBedA || !haveBedB) { cerr << endl << "*****" << endl << "*****ERROR: Need -a and -b files. " << endl << "*****" << endl; showHelp = true; } if (sameStrand && diffStrand) { cerr << endl << "*****" << endl << "*****ERROR: Request either -s OR -S, not both." << endl << "*****" << endl; showHelp = true; } if (!showHelp) { BedSubtract *bs = new BedSubtract(bedAFile, bedBFile, overlapFraction, sameStrand, diffStrand, removeAll); delete bs; return 0; } else { subtract_help(); } return 0; } void subtract_help(void) { cerr << "\nTool: bedtools subtract (aka subtractBed)" << endl; cerr << "Version: " << VERSION << "\n"; cerr << "Summary: Removes the portion(s) of an interval that is overlapped" << endl; cerr << "\t by another feature(s)." << endl << endl; cerr << "Usage: " << PROGRAM_NAME << " [OPTIONS] -a -b " << endl << endl; cerr << "Options: " << endl; cerr << "\t-f\t" << "Minimum overlap required as a fraction of A." << endl; cerr << "\t\t- Default is 1E-9 (i.e., 1bp)." << endl; cerr << "\t\t- (FLOAT) (e.g. 0.50)" << endl << endl; cerr << "\t-s\t" << "Require same strandedness. That is, only subtract hits in B" << endl; cerr << "\t\tthat overlap A on the _same_ strand." << endl; cerr << "\t\t- By default, overlaps are subtracted without respect to strand." << endl << endl; cerr << "\t-S\t" << "Force strandedness. That is, only subtract hits in B that" << endl; cerr << "\t\toverlap A on the _opposite_ strand." << endl; cerr << "\t\t- By default, overlaps are subtracted without respect to strand." << endl << endl; cerr << "\t-A\t" << "Remove entire feature if any overlap. That is, by default," << endl; cerr << "\t\tonly subtract the portion of A that overlaps B. Here, if" << endl; cerr << "\t\tany overlap is found (or -f amount), the entire feature is removed." << endl << endl; // end the program here exit(1); }