# $Id: IO.pm 16123 2009-09-17 12:57:27Z cjfields $ # # BioPerl module for Bio::Assembly::IO # # based on the Bio::SeqIO module # by Ewan Birney # and Lincoln Stein # # Copyright Robson Francisco de Souza # # You may distribute this module under the same terms as perl itself # # _history # POD documentation - main docs before the code =head1 NAME Bio::Assembly::IO - Handler for Assembly::IO Formats =head1 SYNOPSIS use Bio::Assembly::IO; $in = Bio::Assembly::IO->new(-file=>"'phrap'); $out = Bio::Assembly::IO->new(-file=>">outputfilename", -format=>'phrap'); while ( my $scaffold = $in->next_assembly() ) { # do something with Bio::Assembly::Scaffold instance # ... $out->write_assembly(-scaffold => $scaffold); } $in->close; $out->close; =head1 DESCRIPTION Bio::Assembly::IO is a handler module for formats in the Assembly::IO set (e.g. Bio::Assembly::IO::phrap). =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing lists Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHOR Robson Francisco de Souza E-mail: rfsouza@citri.iq.usp.br =head1 CONTRIBUTORS # =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut package Bio::Assembly::IO; use strict; use base qw(Bio::Root::Root Bio::Root::IO); =head2 new Title : new Usage : Bio::Assembly::IO->new(-file =>$filename,-format=>'format') Function: Returns a new assembly stream Returns : A Bio::Assembly::IO::Handler initialised with the appropriate format Args : -file => $filename -format => format =cut sub new { my ($caller,@args) = @_; my $class = ref($caller) || $caller; # or do we want to call SUPER on an object if $caller is an # object? if( $class =~ /Bio::Assembly::IO::(\S+)/ ) { my ($self) = $class->SUPER::new(@args); $self->_initialize(@args); return $self; } else { my %param = @args; @param{ map { lc $_ } keys %param } = values %param; # lowercase keys $class->throw("Need at least a file name to proceed!") unless (defined $param{'-file'} || defined $ARGV[0]); my $format = $param{'-format'} || $class->_guess_format( $param{-file} || $ARGV[0] ); $format = "\L$format"; # normalize capitalization to lower case # normalize capitalization return unless( $class->_load_format_module($format) ); return "Bio::Assembly::IO::$format"->new(@args); } } # _initialize is chained for all SeqIO classes sub _initialize { my($self, @args) = @_; # initialize the IO part $self->_initialize_io(@args); } =head2 next_assembly Title : next_assembly Usage : $cluster = $stream->next_assembly() Function: Reads the next assembly object from the stream and returns it. Returns : a Bio::Assembly::ScaffoldI compliant object Args : none =cut sub next_assembly { my ($self, $seq) = @_; $self->throw("Sorry, you cannot read from a generic Bio::Assembly::IO object."); } =head2 write_assembly Title : write_assembly Usage : $ass_io->write_assembly($assembly) Function: Write the assembly object in Phrap compatible ACE format Returns : 1 on success, 0 for error Args : A Bio::Assembly::Scaffold object =cut sub write_assembly { shift->throw_not_implemented; } =head2 _load_format_module Title : _load_format_module Usage : *INTERNAL Assembly::IO stuff* Function: Loads up (like use) a module at run time on demand Example : Returns : Args : =cut sub _load_format_module { my ($self,$format) = @_; my $module = "Bio::Assembly::IO::" . $format; my $ok; eval { $ok = $self->_load_module($module); }; if ( $@ ) { print STDERR <_guess_format($filename) Function: guess format based on file suffix Example : Returns : guessed format of filename (lower case) Args : Notes : formats that _filehandle() will guess includes only phrap, by now. =cut sub _guess_format { my $class = shift; my $arg = shift; return unless defined($arg); return 'ace' if ($arg =~ /\.ace\.\d+$/i); return 'phrap' if ($arg =~ /\.phrap\.out$/i); } sub DESTROY { my $self = shift; $self->close(); } # I need some direction on these!! The module works so I haven't fiddled with them! # Me neither! (rfsouza) sub TIEHANDLE { my ($class,$val) = @_; return bless {'seqio' => $val}, $class; } sub READLINE { my $self = shift; return $self->{'seqio'}->next_seq() unless wantarray; my (@list, $obj); push @list, $obj while $obj = $self->{'seqio'}->next_seq(); return @list; } sub PRINT { my $self = shift; $self->{'seqio'}->write_seq(@_); } 1;