# # Ensembl module for Bio::EnsEMBL::DBSQL::Funcgen::ExternalFeatureAdaptor # =head1 LICENSE Copyright (c) 1999-2013 The European Bioinformatics Institute and Genome Research Limited. All rights reserved. This software is distributed under a modified Apache license. For license details, please see http://www.ensembl.org/info/about/code_licence.html =head1 CONTACT Please email comments or questions to the public Ensembl developers list at . Questions may also be sent to the Ensembl help desk at . =head1 NAME Bio::EnsEMBL::DBSQL::Funcgen::ExternalFeatureAdaptor - A database adaptor for fetching and storing ExternalFeature objects. =head1 SYNOPSIS my $afa = $db->get_ExternalFeatureAdaptor(); my $features = $afa->fetch_all_by_Slice($slice); =head1 DESCRIPTION The ExternalFeatureAdaptor is a database adaptor for storing and retrieving ExternalFeature objects. =cut use strict; use warnings; package Bio::EnsEMBL::Funcgen::DBSQL::ExternalFeatureAdaptor; use Bio::EnsEMBL::Utils::Exception qw( throw warning ); use Bio::EnsEMBL::Funcgen::ExternalFeature; use Bio::EnsEMBL::Funcgen::DBSQL::SetFeatureAdaptor; use vars qw(@ISA); @ISA = qw(Bio::EnsEMBL::Funcgen::DBSQL::SetFeatureAdaptor); =head2 _tables Args : None Example : None Description: PROTECTED implementation of superclass abstract method. Returns the names and aliases of the tables to use for queries. Returntype : List of listrefs of strings Exceptions : None Caller : Internal Status : At Risk =cut sub _tables { my $self = shift; return ( [ 'external_feature', 'ef' ], [ 'feature_set', 'fs' ],#this is required for fetching on analysis, external_db (cell_type or feature_type). ); } =head2 _columns Args : None Example : None Description: PROTECTED implementation of superclass abstract method. Returns a list of columns to use for queries. Returntype : List of strings Exceptions : None Caller : Internal Status : At Risk =cut sub _columns { my $self = shift; return qw( ef.external_feature_id ef.seq_region_id ef.seq_region_start ef.seq_region_end ef.seq_region_strand ef.display_label ef.feature_type_id ef.feature_set_id ef.interdb_stable_id ); } =head2 _objs_from_sth Arg [1] : DBI statement handle object Example : None Description: PROTECTED implementation of superclass abstract method. Creates ExternalFeature objects from an executed DBI statement handle. Returntype : Listref of Bio::EnsEMBL::ExternalFeature objects Exceptions : None Caller : Internal Status : At Risk =cut sub _objs_from_sth { my ($self, $sth, $mapper, $dest_slice) = @_; #For EFG this has to use a dest_slice from core/dnaDB whether specified or not. #So if it not defined then we need to generate one derived from the species_name and schema_build of the feature we're retrieving. my ($sa, @features, $seq_region_id, %fset_hash, %slice_hash, %sr_name_hash, %sr_cs_hash, %ftype_hash); $sa = $dest_slice->adaptor->db->get_SliceAdaptor() if($dest_slice);#don't really need this if we're using DNADBSliceAdaptor? $sa ||= $self->db->get_SliceAdaptor(); my $fset_adaptor = $self->db->get_FeatureSetAdaptor(); my $ftype_adaptor = $self->db->get_FeatureTypeAdaptor(); my ( $external_feature_id, $efg_seq_region_id, $seq_region_start, $seq_region_end, $seq_region_strand, $fset_id, $display_label, $ftype_id, $interdb_stable_id ); $sth->bind_columns( \$external_feature_id, \$efg_seq_region_id, \$seq_region_start, \$seq_region_end, \$seq_region_strand, \$display_label, \$ftype_id, \$fset_id, \$interdb_stable_id ); #abstract to BaseFeatureAdaptor? my ($asm_cs, $cmp_cs, $asm_cs_name, $asm_cs_vers, $cmp_cs_name, $cmp_cs_vers); if ($mapper) { $asm_cs = $mapper->assembled_CoordSystem(); $cmp_cs = $mapper->component_CoordSystem(); $asm_cs_name = $asm_cs->name(); $asm_cs_vers = $asm_cs->version(); $cmp_cs_name = $cmp_cs->name(); $cmp_cs_vers = $cmp_cs->version(); } my ($dest_slice_start, $dest_slice_end, $dest_slice_strand, $dest_slice_length, $dest_slice_sr_name); if ($dest_slice) { $dest_slice_start = $dest_slice->start(); $dest_slice_end = $dest_slice->end(); $dest_slice_strand = $dest_slice->strand(); $dest_slice_length = $dest_slice->length(); $dest_slice_sr_name = $dest_slice->seq_region_name(); } FEATURE: while ( $sth->fetch() ) { #Need to build a slice adaptor cache here? #Would only ever want to do this if we enable mapping between assemblies?? #Or if we supported the mapping between cs systems for a given schema_build, #which would have to be handled by the core api #get core seq_region_id $seq_region_id = $self->get_core_seq_region_id($efg_seq_region_id); if(! $seq_region_id){ warn "Cannot get slice for eFG seq_region_id $efg_seq_region_id\n". "The region you are using is not present in the current dna DB"; next; } #Get the FeatureSet/Type objects $fset_hash{$fset_id} = $fset_adaptor->fetch_by_dbID($fset_id) if(! exists $fset_hash{$fset_id}); $ftype_hash{$ftype_id} = $ftype_adaptor->fetch_by_dbID($ftype_id) if(! exists $ftype_hash{$ftype_id}); # Get the slice object my $slice = $slice_hash{'ID:'.$seq_region_id}; if (! $slice) { $slice = $sa->fetch_by_seq_region_id($seq_region_id); $slice_hash{'ID:'.$seq_region_id} = $slice; $sr_name_hash{$seq_region_id} = $slice->seq_region_name(); $sr_cs_hash{$seq_region_id} = $slice->coord_system(); } my $sr_name = $sr_name_hash{$seq_region_id}; my $sr_cs = $sr_cs_hash{$seq_region_id}; #abstract to BaseFeatureAdaptor? # Remap the feature coordinates to another coord system if a mapper was provided if ($mapper) { throw("Not yet implmented mapper, check equals are Funcgen calls too!"); ($sr_name, $seq_region_start, $seq_region_end, $seq_region_strand) = $mapper->fastmap($sr_name, $seq_region_start, $seq_region_end, $seq_region_strand, $sr_cs); # Skip features that map to gaps or coord system boundaries next FEATURE if !defined $sr_name; # Get a slice in the coord system we just mapped to if ( $asm_cs == $sr_cs || ( $cmp_cs != $sr_cs && $asm_cs->equals($sr_cs) ) ) { $slice = $slice_hash{"NAME:$sr_name:$cmp_cs_name:$cmp_cs_vers"} ||= $sa->fetch_by_region($cmp_cs_name, $sr_name, undef, undef, undef, $cmp_cs_vers); } else { $slice = $slice_hash{"NAME:$sr_name:$asm_cs_name:$asm_cs_vers"} ||= $sa->fetch_by_region($asm_cs_name, $sr_name, undef, undef, undef, $asm_cs_vers); } } # If a destination slice was provided convert the coords # If the destination slice starts at 1 and is forward strand, nothing needs doing if ($dest_slice) { unless ($dest_slice_start == 1 && $dest_slice_strand == 1) { if ($dest_slice_strand == 1) { $seq_region_start = $seq_region_start - $dest_slice_start + 1; $seq_region_end = $seq_region_end - $dest_slice_start + 1; } else { my $tmp_seq_region_start = $seq_region_start; $seq_region_start = $dest_slice_end - $seq_region_end + 1; $seq_region_end = $dest_slice_end - $tmp_seq_region_start + 1; $seq_region_strand *= -1; } } # Throw away features off the end of the requested slice next FEATURE if $seq_region_end < 1 || $seq_region_start > $dest_slice_length || ( $dest_slice_sr_name ne $sr_name ); $slice = $dest_slice; } push @features, Bio::EnsEMBL::Funcgen::ExternalFeature->new_fast ({ 'start' => $seq_region_start, 'end' => $seq_region_end, 'strand' => $seq_region_strand, 'slice' => $slice, 'analysis' => $fset_hash{$fset_id}->analysis(), 'adaptor' => $self, 'dbID' => $external_feature_id, 'display_label' => $display_label, 'set' => $fset_hash{$fset_id}, 'feature_type' => $ftype_hash{$ftype_id}, 'interdb_stable_id', => $interdb_stable_id, }); } return \@features; } =head2 store Args : List of Bio::EnsEMBL::Funcgen::ExternalFeature objects Example : $ofa->store(@features); Description: Stores given ExternalFeature objects in the database. Should only be called once per feature because no checks are made for duplicates. Sets dbID and adaptor on the objects that it stores. Returntype : Arrayref of stored ExternalFeatures Exceptions : Throws if a list of ExternalFeature objects is not provided or if the Analysis, CellType and FeatureType objects are not attached or stored Caller : General Status : At Risk =cut sub store{ my ($self, @efs) = @_; if (scalar(@efs) == 0) { throw('Must call store with a list of ExternalFeature objects'); } my $sth = $self->prepare(" INSERT INTO external_feature ( seq_region_id, seq_region_start, seq_region_end, seq_region_strand, display_label, feature_type_id, feature_set_id ) VALUES (?, ?, ?, ?, ?, ?, ?) "); my $db = $self->db(); FEATURE: foreach my $ef (@efs) { if(! (ref($ef) && $ef->isa('Bio::EnsEMBL::Funcgen::ExternalFeature') )) { throw('Feature must be an ExternalFeature object'); } if ( $ef->is_stored($db) ) { #does not accomodate adding Feature to >1 feature_set warning('ExternalFeature [' . $ef->dbID() . '] is already stored in the database'); next FEATURE; } $self->db->is_stored_and_valid('Bio::EnsEMBL::Funcgen::FeatureSet', $ef->feature_set); my $seq_region_id; ($ef, $seq_region_id) = $self->_pre_store($ef); $sth->bind_param(1, $seq_region_id, SQL_INTEGER); $sth->bind_param(2, $ef->start(), SQL_INTEGER); $sth->bind_param(3, $ef->end(), SQL_INTEGER); $sth->bind_param(4, $ef->strand(), SQL_TINYINT); $sth->bind_param(5, $ef->display_label(), SQL_VARCHAR); $sth->bind_param(6, $ef->feature_type->dbID(), SQL_INTEGER); $sth->bind_param(7, $ef->feature_set->dbID(), SQL_INTEGER); $sth->execute(); $ef->dbID( $sth->{'mysql_insertid'} ); $ef->adaptor($self); $self->store_associated_feature_types($ef) if (defined $ef->{'associated_feature_types'}); } return \@efs; } =head2 fetch_by_interdb_stable_id Arg [1] : Integer $stable_id - The 'interdb stable id' of the ExternalFeature to retrieve Example : my $rf = $rf_adaptor->fetch_by_interdb_stable_id(1); Description: Retrieves a ExternalFeature via its interdb_stable id. This is really an internal method to facilitate inter DB linking. Returntype : Bio::EnsEMBL::Funcgen::ExternalFeature Exceptions : none Caller : general Status : Stable =cut sub fetch_by_interdb_stable_id { my ($self, $stable_id) = @_; $self->bind_param_generic_fetch($stable_id, SQL_INTEGER); return $self->generic_fetch('ef.interdb_stable_id=?')->[0]; } 1;