# # Ensembl module for Bio::EnsEMBL::DBSQL::Funcgen::SegmentationFeatureAdaptor # =head1 LICENSE Copyright (c) 1999-2013 The European Bioinformatics Institute and Genome Research Limited. All rights reserved. This software is distributed under a modified Apache license. For license details, please see http://www.ensembl.org/info/about/code_licence.html =head1 CONTACT Please email comments or questions to the public Ensembl developers list at . Questions may also be sent to the Ensembl help desk at . =head1 NAME Bio::EnsEMBL::DBSQL::Funcgen::SegmentationFeatureAdaptor - A database adaptor for fetching and storing SegmentationFeature objects. =head1 SYNOPSIS my $afa = $db->get_SegmentationFeatureAdaptor(); my $features = $afa->fetch_all_by_Slice($slice); =head1 DESCRIPTION The SegmentationFeatureAdaptor is a database adaptor for storing and retrieving SegmentationFeature objects. =cut package Bio::EnsEMBL::Funcgen::DBSQL::SegmentationFeatureAdaptor; use strict; use warnings; use Bio::EnsEMBL::Utils::Exception qw( throw warning ); use Bio::EnsEMBL::Funcgen::SegmentationFeature; use Bio::EnsEMBL::Funcgen::DBSQL::SetFeatureAdaptor; use vars qw(@ISA); @ISA = qw(Bio::EnsEMBL::Funcgen::DBSQL::SetFeatureAdaptor); #This adaptor does not yet use query extension =head2 _tables Args : None Example : None Description: PROTECTED implementation of superclass abstract method. Returns the names and aliases of the tables to use for queries. Returntype : List of listrefs of strings Exceptions : None Caller : Internal Status : At Risk =cut sub _tables { my $self = shift; return ( ['segmentation_feature', 'sf'], ['feature_set', 'fs'] ); } =head2 _columns Args : None Example : None Description: PROTECTED implementation of superclass abstract method. Returns a list of columns to use for queries. Returntype : List of strings Exceptions : None Caller : Internal Status : At Risk =cut sub _columns { my $self = shift; return qw( sf.segmentation_feature_id sf.seq_region_id sf.seq_region_start sf.seq_region_end sf.seq_region_strand sf.feature_type_id sf.feature_set_id sf.score sf.display_label ); } =head2 _objs_from_sth Arg [1] : DBI statement handle object Example : None Description: PROTECTED implementation of superclass abstract method. Creates SegmentationFeature objects from an executed DBI statement handle. Returntype : Listref of Bio::EnsEMBL::SegmentationFeature objects Exceptions : None Caller : Internal Status : At Risk =cut sub _objs_from_sth { my ($self, $sth, $mapper, $dest_slice) = @_; #For EFG this has to use a dest_slice from core/dnaDB whether specified or not. #So if it not defined then we need to generate one derived from the species_name and schema_build of the feature we're retrieving. my ($sa, $seq_region_id); #don't really need this if we're using DNADBSliceAdaptor? $sa = $dest_slice->adaptor->db->get_SliceAdaptor() if($dest_slice); $sa ||= $self->db->dnadb->get_SliceAdaptor(); #Some of this in now probably overkill as we'll always be using the DNADB as the slice DB #Hence it should always be on the same coord system my $fset_adaptor = $self->db->get_FeatureSetAdaptor; my $ftype_adaptor = $self->db->get_FeatureTypeAdaptor; my @features; my (%fset_hash, %slice_hash, %sr_name_hash, %sr_cs_hash, %ftype_hash); my ( $segmentation_feature_id, $efg_seq_region_id, $seq_region_start, $seq_region_end, $seq_region_strand, $ftype_id, $fset_id, $score, $display_label ); $sth->bind_columns( \$segmentation_feature_id, \$efg_seq_region_id, \$seq_region_start, \$seq_region_end, \$seq_region_strand, \$ftype_id, \$fset_id, \$score, \$display_label, ); my $asm_cs; my $cmp_cs; my $asm_cs_name; my $asm_cs_vers; my $cmp_cs_name; my $cmp_cs_vers; if ($mapper) { $asm_cs = $mapper->assembled_CoordSystem(); $cmp_cs = $mapper->component_CoordSystem(); $asm_cs_name = $asm_cs->name(); $asm_cs_vers = $asm_cs->version(); $cmp_cs_name = $cmp_cs->name(); $cmp_cs_vers = $cmp_cs->version(); } my $dest_slice_start; my $dest_slice_end; my $dest_slice_strand; my $dest_slice_length; my $dest_slice_sr_name; if ($dest_slice) { $dest_slice_start = $dest_slice->start(); $dest_slice_end = $dest_slice->end(); $dest_slice_strand = $dest_slice->strand(); $dest_slice_length = $dest_slice->length(); $dest_slice_sr_name = $dest_slice->seq_region_name(); } FEATURE: while ( $sth->fetch() ) { #Need to build a slice adaptor cache here? #Would only ever want to do this if we enable mapping between assemblies?? #Or if we supported the mapping between cs systems for a given schema_build, which would have to be handled by the core api #get core seq_region_id #This fails if we are using a 'comparable' CoordSystem as we don't have a cache #for the new DB. Wasn't this fixed with the tmp seq_region_cache? $seq_region_id = $self->get_core_seq_region_id($efg_seq_region_id); if(! $seq_region_id){ warn "Cannot get slice for eFG seq_region_id $efg_seq_region_id\n". "The region you are using is not present in the current seq_region_cache.\n". "Maybe you need to redefine the dnadb or update_DB_for_release?"; next; } #Get the FeatureSet object $fset_hash{$fset_id} = $fset_adaptor->fetch_by_dbID($fset_id) if ! exists $fset_hash{$fset_id}; $ftype_hash{$ftype_id} = $ftype_adaptor->fetch_by_dbID($ftype_id) if ! exists $fset_hash{$ftype_id}; # Get the slice object my $slice = $slice_hash{'ID:'.$seq_region_id}; if (! $slice) { $slice = $sa->fetch_by_seq_region_id($seq_region_id); $slice_hash{'ID:'.$seq_region_id} = $slice; $sr_name_hash{$seq_region_id} = $slice->seq_region_name(); $sr_cs_hash{$seq_region_id} = $slice->coord_system(); } my $sr_name = $sr_name_hash{$seq_region_id}; my $sr_cs = $sr_cs_hash{$seq_region_id}; # Remap the feature coordinates to another coord system if a mapper was provided if ($mapper) { throw("Not yet implmented mapper, check equals are Funcgen calls too!"); ($sr_name, $seq_region_start, $seq_region_end, $seq_region_strand) = $mapper->fastmap($sr_name, $seq_region_start, $seq_region_end, $seq_region_strand, $sr_cs); # Skip features that map to gaps or coord system boundaries next FEATURE if !defined $sr_name; # Get a slice in the coord system we just mapped to if ( $asm_cs == $sr_cs || ( $cmp_cs != $sr_cs && $asm_cs->equals($sr_cs) ) ) { $slice = $slice_hash{"NAME:$sr_name:$cmp_cs_name:$cmp_cs_vers"} ||= $sa->fetch_by_region($cmp_cs_name, $sr_name, undef, undef, undef, $cmp_cs_vers); } else { $slice = $slice_hash{"NAME:$sr_name:$asm_cs_name:$asm_cs_vers"} ||= $sa->fetch_by_region($asm_cs_name, $sr_name, undef, undef, undef, $asm_cs_vers); } } # If a destination slice was provided convert the coords # If the destination slice starts at 1 and is forward strand, nothing needs doing if ($dest_slice) { unless ($dest_slice_start == 1 && $dest_slice_strand == 1) { if ($dest_slice_strand == 1) { $seq_region_start = $seq_region_start - $dest_slice_start + 1; $seq_region_end = $seq_region_end - $dest_slice_start + 1; } else { my $tmp_seq_region_start = $seq_region_start; $seq_region_start = $dest_slice_end - $seq_region_end + 1; $seq_region_end = $dest_slice_end - $tmp_seq_region_start + 1; $seq_region_strand *= -1; } } # Throw away features off the end of the requested slice if(! $self->force_reslice){ #force_reslice set by RegulatoryFeature::regulatory_attributes #so we don't lose attrs which are not on the dest_slice next FEATURE if $seq_region_end < 1 || $seq_region_start > $dest_slice_length || ( $dest_slice_sr_name ne $sr_name ); $slice = $dest_slice; } } push @features, Bio::EnsEMBL::Funcgen::SegmentationFeature->new_fast ( { start => $seq_region_start, end => $seq_region_end, strand => $seq_region_strand, slice => $slice, #'analysis' => $fset_hash{$fset_id}->analysis(), #Need to pass this to keep Feature.pm happy #Let's grab this from the FeatureSet in SetFeature new and pass score => $score, adaptor => $self, dbID => $segmentation_feature_id, display_label => $display_label, set => $fset_hash{$fset_id}, feature_type => $ftype_hash{$ftype_id}, } ); } return \@features; } =head2 store Args : List of Bio::EnsEMBL::Funcgen::SegmentationFeature objects Example : $ofa->store(@features); Description: Stores given SegmentationFeature objects in the database. Should only be called once per feature because no checks are made for duplicates. Sets dbID and adaptor on the objects that it stores. Returntype : Listref of stored SegmentationFeatures Exceptions : Throws if a list of SegmentationFeature objects is not provided or if the Analysis, CellType and FeatureType objects are not attached or stored Caller : General Status : At Risk =cut sub store{ my ($self, @segs) = @_; if (scalar(@segs) == 0) { throw('Must call store with a list of SegmentationFeature objects'); } my $sth = $self->prepare(" INSERT INTO segmentation_feature ( seq_region_id, seq_region_start, seq_region_end, seq_region_strand, feature_type_id, feature_set_id, score, display_label ) VALUES (?, ?, ?, ?, ?, ?, ?, ?) "); my $db = $self->db(); FEATURE: foreach my $seg (@segs) { if( !ref $seg || !$seg->isa('Bio::EnsEMBL::Funcgen::SegmentationFeature') ) { throw('Feature must be an SegmentationFeature object'); } if ( $seg->is_stored($db) ) { #does not accomodate adding Feature to >1 feature_set warning('SegmentationFeature [' . $seg->dbID() . '] is already stored in the database'); next FEATURE; } $self->db->is_stored_and_valid('Bio::EnsEMBL::Funcgen::FeatureSet', $seg->feature_set); my $seq_region_id; ($seg, $seq_region_id) = $self->_pre_store($seg); $sth->bind_param(1, $seq_region_id, SQL_INTEGER); $sth->bind_param(2, $seg->start(), SQL_INTEGER); $sth->bind_param(3, $seg->end(), SQL_INTEGER); $sth->bind_param(4, $seg->strand(), SQL_TINYINT); $sth->bind_param(5, $seg->feature_type->dbID(), SQL_INTEGER); $sth->bind_param(6, $seg->feature_set->dbID(), SQL_INTEGER); $sth->bind_param(7, $seg->score(), SQL_DOUBLE); $sth->bind_param(8, $seg->display_label(), SQL_VARCHAR); $sth->execute(); $seg->dbID( $sth->{'mysql_insertid'} ); $seg->adaptor($self); } return \@segs; } 1;