=pod =head1 NAME Bio::FeatureIO::bed - read/write features from UCSC BED format =head1 SYNOPSIS my $in = Bio::FeatureIO(-format => 'bed', -file => 'file.bed'); for my $feat ($in->next_feature) { # do something with $feat (a Bio::SeqFeature::Annotated object) } my $out = Bio::FeatureIO(-format=>'bed'); for my $feat ($seq->get_seqFeatures) { $out->write_feature($feat); } =head1 DESCRIPTION See L. Currently for read and write only the first 6 fields (chr, start, end, name, score, strand) are supported. =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHOR - Allen Day Email allenday@ucla.edu =head1 CONTRIBUTORS Sendu Bala, bix@sendu.me.uk =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::FeatureIO::bed; use strict; use base qw(Bio::FeatureIO); use Bio::SeqFeature::Annotated; use Bio::Annotation::SimpleValue; use Bio::OntologyIO; use Scalar::Util qw(looks_like_number); =head2 _initialize Title : _initialize Function: initializes BED for reading/writing Args : all optional: name description ---------------------------------------------------------- -name the name for the BED track, stored in header name defaults to localtime() -description the description for the BED track, stored in header. defaults to localtime(). -use_score whether or not the score attribute of features should be used when rendering them. the higher the score the darker the color. defaults to 0 (false) =cut sub _initialize { my($self,%arg) = @_; $self->SUPER::_initialize(%arg); $self->name($arg{-name} || scalar(localtime())); $self->description($arg{-description} || scalar(localtime())); $self->use_score($arg{-use_score} || 0); $self->_print(sprintf('track name="%s" description="%s" useScore=%d', $self->name, $self->description, $self->use_score ? 1 : 0 )."\n") if $self->mode eq 'w'; } =head2 use_score Title : use_score Usage : $obj->use_score($newval) Function: should score be used to adjust feature color when rendering? set to true if so. Example : Returns : value of use_score (a scalar) Args : on set, new value (a scalar or undef, optional) =cut sub use_score{ my $self = shift; return $self->{'use_score'} = shift if @_; return $self->{'use_score'}; } =head2 name Title : name Usage : $obj->name($newval) Function: name of BED track Example : Returns : value of name (a scalar) Args : on set, new value (a scalar or undef, optional) =cut sub name{ my $self = shift; return $self->{'name'} = shift if @_; return $self->{'name'}; } =head2 description Title : description Usage : $obj->description($newval) Function: description of BED track Example : Returns : value of description (a scalar) Args : on set, new value (a scalar or undef, optional) =cut sub description{ my $self = shift; return $self->{'description'} = shift if @_; return $self->{'description'}; } sub write_feature { my($self,$feature) = @_; $self->throw("only Bio::SeqFeature::Annotated objects are writeable") unless $feature->isa('Bio::SeqFeature::Annotated'); my $chrom = $feature->seq_id || ''; my $chrom_start = $feature->start || 0; # output start is supposed to be 0-based my $chrom_end = ($feature->end + 1) || 1; # output end is supposed to not be part of the feature #try to make a reasonable name my $name = undef; my @v; if (@v = ($feature->annotation->get_Annotations('Name'))){ $name = $v[0]; $self->warn("only using first of feature's multiple names: ".join ',', map {$_->value} @v) if scalar(@v) > 1; } elsif (@v = ($feature->annotation->get_Annotations('ID'))){ $name = $v[0]; $self->warn("only using first of feature's multiple IDs: ".join ',', map {$_->value} @v) if scalar(@v) > 1; } else { $name = 'anonymous'; } if (ref($name)) { $name = $name->value; } if (ref($chrom)) { $chrom = $chrom->value; } my $score = $feature->score || 0; my $strand = $feature->strand == 0 ? '-' : '+'; #default to + my $thick_start = ''; #not implemented, used for CDS my $thick_end = ''; #not implemented, used for CDS my $reserved = 0; my $block_count = ''; #not implemented, used for sub features my $block_sizes = ''; #not implemented, used for sub features my $block_starts = ''; #not implemented, used for sub features $self->_print(join("\t",($chrom,$chrom_start,$chrom_end,$name,$score,$strand,$thick_start,$thick_end,$reserved,$block_count,$block_sizes, $block_starts))."\n"); $self->write_feature($_) foreach $feature->get_SeqFeatures(); } sub next_feature { my $self = shift; my $line = $self->_readline || return; my ($seq_id, $start, $end, $name, $score, $strand) = split(/\s+/, $line); $strand ||= '+'; unless (looks_like_number($start) && looks_like_number($end)) { # skip what is probably a header line return $self->next_feature; } my $feature = Bio::SeqFeature::Annotated->new(-start => $start, # start is 0 based -end => --$end, # end is not part of the feature $score ? (-score => $score) : (), $strand ? (-strand => $strand eq '+' ? 1 : -1) : ()); $feature->seq_id($seq_id); if ($name) { my $sv = Bio::Annotation::SimpleValue->new(-tagname => 'Name', -value => $name); $feature->annotation->add_Annotation($sv); } return $feature; } 1;