=head1 NAME Bio::FeatureIO::interpro - read features from InterPro XML =head1 SYNOPSIS my $in = Bio::FeatureIO(-format=>'interpro'); while (my $feat = $in->next_feature) { # do something with the Bio::SeqFeatureI object } =head1 DESCRIPTION See L. =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHOR - Allen Day Email allenday@ucla.edu =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::FeatureIO::interpro; use strict; use base qw(Bio::FeatureIO); use Bio::SeqFeature::Annotated; use Bio::OntologyIO; use Bio::Annotation::Comment; use Bio::Annotation::DBLink; use Bio::Annotation::OntologyTerm; use Bio::Annotation::SimpleValue; use Bio::Annotation::Target; use URI::Escape; use XML::DOM; use XML::DOM::XPath; sub _initialize { my($self,%arg) = @_; $self->SUPER::_initialize(%arg); $self->xml_parser(XML::DOM::Parser->new()); my $buf; while(($buf = $self->_readline()) && $buf !~ /_pushback($buf); } sub next_feature { my $self =shift; my $buf; #line buffer my $ok = 0; #true if there is another record in stream my $record; #holds the record to be parsed and returned. #try to dump buffer from last record before moving on to next record my $f = $self->_shift_feature_buffer(); if($f){ return $f; } while(my $buf = $self->_readline()){ $ok = 1 if $buf =~ m!!; } return unless $ok; my $dom = $self->xml_parser->parse($record); my ($pNode) = $dom->findnodes('/protein'); my @iNodes = $pNode->findnodes('/protein/interpro'); foreach my $iNode (@iNodes){ my @cNodes = $iNode->findnodes('classification'); my @mNodes = $iNode->findnodes('match'); #we don't handle these #my @nNodes = $iNode->findnodes('contains'); #my @fNodes = $iNode->findnodes('found_in'); foreach my $mNode (@mNodes){ my @lNodes = $mNode->findnodes('location'); foreach my $lNode (@lNodes){ my $feature = Bio::SeqFeature::Annotated->new( -start => $lNode->getAttribute('start'), -end => $lNode->getAttribute('end'), -score => $lNode->getAttribute('score'), # -seq_id => $pNode->getAttribute('id'), ); $feature->seq_id->value($pNode->getAttribute('id')); #warn $pNode->getAttribute('id'); $feature->source( $lNode->getAttribute('evidence') ); my $t = Bio::Annotation::OntologyTerm->new(-identifier => 'SO:0000417', -name => 'polypeptide_domain'); $feature->add_Annotation('type',$t); my $c = Bio::Annotation::Comment->new(-tagname => 'comment', -text => $iNode->getAttribute('name')); $feature->add_Annotation($c); my $d = Bio::Annotation::DBLink->new(); $d->database($mNode->getAttribute('dbname')); $d->primary_id($mNode->getAttribute('id')); $d->optional_id($mNode->getAttribute('name')); $feature->annotation->add_Annotation('dblink',$d); my $s = Bio::Annotation::SimpleValue->new(-tagname => 'status', -value => $lNode->getAttribute('status')); $feature->annotation->add_Annotation($s); foreach my $cNode (@cNodes){ my $o = Bio::Annotation::OntologyTerm->new(-identifier => $cNode->getAttribute('id')); $feature->annotation->add_Annotation('ontology_term',$o); } $self->_push_feature_buffer($feature); } } } return $self->_shift_feature_buffer; } =head2 _push_feature_buffer() Usage : Function: Returns : Args : =cut sub _push_feature_buffer { my ($self,$f) = @_; if(ref($f)){ push @{ $self->{feature_buffer} }, $f; } } =head2 _shift_feature_buffer() Usage : Function: Returns : Args : =cut sub _shift_feature_buffer { my ($self) = @_; return $self->{feature_buffer} ? shift @{ $self->{feature_buffer} } : undef; } =head2 xml_parser() Usage : $obj->xml_parser($newval) Function: Example : Returns : value of xml_parser (a scalar) Args : on set, new value (a scalar or undef, optional) =cut sub xml_parser { my($self,$val) = @_; $self->{'xml_parser'} = $val if defined($val); return $self->{'xml_parser'}; } 1;