# # $Id: Fasta.pm 16168 2009-09-25 21:07:32Z cjfields $ # # BioPerl module for Bio::Index::Fasta # # Please direct questions and support issues to # # Cared for by James Gilbert # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code =head1 NAME Bio::Index::Fasta - Interface for indexing (multiple) fasta files =head1 SYNOPSIS # Make an index for one or more fasta files use Bio::Index::Fasta; use strict; my $Index_File_Name = shift; my $inx = Bio::Index::Fasta->new(-filename => $Index_File_Name, -write_flag => 1); $inx->make_index(@ARGV); # Once the index is made it can accessed, either in the # same script or a different one use Bio::Index::Fasta; use strict; my $Index_File_Name = shift; my $inx = Bio::Index::Fasta->new(-filename => $Index_File_Name); my $out = Bio::SeqIO->new(-format => 'Fasta', -fh => \*STDOUT); foreach my $id (@ARGV) { my $seq = $inx->fetch($id); # Returns Bio::Seq object $out->write_seq($seq); } # or, alternatively my $id; my $seq = $inx->get_Seq_by_id($id); # identical to fetch() =head1 DESCRIPTION Inherits functions for managing dbm files from Bio::Index::Abstract.pm, and provides the basic funtionallity for indexing fasta files, and retrieving the sequence from them. For best results 'use strict'. Bio::Index::Fasta supports the Bio::DB::BioSeqI interface, meaning it can be used as a Sequence database for other parts of bioperl Additional example code is available in scripts/index/*PLS and in the Bioperl Tutorial (L) Note that by default the key for the sequence will be the first continuous string after the 'E' in the fasta header. If you want to use a specific substring of the fasta header you must use the id_parser() method. You can also set or customize the unique key used to retrieve by writing your own function and calling the id_parser() method. For example: $inx->id_parser(\&get_id); # make the index $inx->make_index($file_name); # here is where the retrieval key is specified sub get_id { my $line = shift; $line =~ /^>.+gi\|(\d+)/; $1; } =head1 FEED_BACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHOR - James Gilbert Email - jgrg@sanger.ac.uk =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::Index::Fasta; use strict; use warnings; use Bio::Seq; use base qw(Bio::Index::AbstractSeq); # # Suggested fix by Michael G Schwern to # get around a clash with CPAN shell... # sub _version { return 0.2; } =head2 _file_format Title : _file_format Function: The file format for this package, which is needed by the SeqIO system when reading the sequence. Returns : 'Fasta' =cut sub _file_format { return 'Fasta'; } =head2 _index_file Title : _index_file Usage : $index->_index_file( $file_name, $i ) Function: Specialist function to index FASTA format files. Is provided with a filename and an integer by make_index in its SUPER class. Example : Returns : Args : =cut sub _index_file { my( $self, $file, # File name $i, # Index-number of file being indexed ) = @_; my( $begin, # Offset from start of file of the start # of the last found record. ); my $id_parser = $self->id_parser; open my $FASTA, '<', $file or $self->throw("Can't open file for read : $file"); # Main indexing loop while (<$FASTA>) { if (/^>/) { # the following was fixed to allow validation - cjfields # $begin is the position of the first character after the '>' my $offset = ( $^O =~ /mswin/i ) ? 1 : 0; $begin = tell($FASTA) - length( $_ ) - $offset; foreach my $id (&$id_parser($_)) { $self->add_record($id, $i, $begin); } } } close $FASTA; return 1; } =head2 id_parser Title : id_parser Usage : $index->id_parser( CODE ) Function: Stores or returns the code used by record_id to parse the ID for record from a string. Useful for (for instance) specifying a different parser for different flavours of FASTA file. Returns \&default_id_parser (see below) if not set. If you supply your own id_parser subroutine, then it should expect a fasta description line. An entry will be added to the index for each string in the list returned. Example : $index->id_parser( \&my_id_parser ) Returns : ref to CODE if called without arguments Args : CODE =cut sub id_parser { my( $self, $code ) = @_; if ($code) { $self->{'_id_parser'} = $code; } return $self->{'_id_parser'} || \&default_id_parser; } =head2 default_id_parser Title : default_id_parser Usage : $id = default_id_parser( $header ) Function: The default Fasta ID parser for Fasta.pm Returns $1 from applying the regexp /^>\s*(\S+)/ to $header. Returns : ID string Args : a fasta header line string =cut sub default_id_parser { if ($_[0] =~ /^>\s*(\S+)/) { return $1; } else { return; } } 1;