# $Id: Atomic.pm 16123 2009-09-17 12:57:27Z cjfields $ # # BioPerl module for Bio::Location::Atomic # Please direct questions and support issues to # # Cared for by Jason Stajich # # Copyright Jason Stajich # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code =head1 NAME Bio::Location::Atomic - Implementation of a Atomic Location on a Sequence =head1 SYNOPSIS use Bio::Location::Atomic; my $location = Bio::Location::Atomic->new(-start => 1, -end => 100, -strand => 1 ); if( $location->strand == -1 ) { printf "complement(%d..%d)\n", $location->start, $location->end; } else { printf "%d..%d\n", $location->start, $location->end; } =head1 DESCRIPTION This is an implementation of Bio::LocationI to manage simple location information on a Sequence. =head1 FEEDBACK User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHOR - Jason Stajich Email jason-at-bioperl-dot-org =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::Location::Atomic; use strict; use Bio::Location::WidestCoordPolicy; use base qw(Bio::Root::Root Bio::LocationI); our $coord_policy = Bio::Location::WidestCoordPolicy->new(); sub new { my ($class, @args) = @_; my $self = {}; # This is for the case when we've done something like this # get a 2 features from somewhere (like Bio::Tools::GFF) # Do # my $location = $f1->location->union($f2->location); # We get an error without the following code which # explictly loads the Bio::Location::Simple class eval { ($class) = ref($class) if ref($class); Bio::Root::Root->_load_module($class); }; if ( $@ ) { Bio::Root::Root->throw("$class cannot be found\nException $@"); } bless $self,$class; my ($v,$start,$end,$strand,$seqid) = $self->_rearrange([qw(VERBOSE START END STRAND SEQ_ID)],@args); defined $v && $self->verbose($v); defined $strand && $self->strand($strand); defined $start && $self->start($start); defined $end && $self->end($end); if( defined $self->start && defined $self->end && $self->start > $self->end && $self->strand != -1 ) { $self->warn("When building a location, start ($start) is expected to be less than end ($end), ". "however it was not. Switching start and end and setting strand to -1"); $self->strand(-1); my $e = $self->end; my $s = $self->start; $self->start($e); $self->end($s); } $seqid && $self->seq_id($seqid); return $self; } =head2 start Title : start Usage : $start = $loc->start(); Function: get/set the start of this range Returns : the start of this range Args : optionaly allows the start to be set : using $loc->start($start) =cut sub start { my ($self, $value) = @_; $self->min_start($value) if( defined $value ); return $self->SUPER::start(); } =head2 end Title : end Usage : $end = $loc->end(); Function: get/set the end of this range Returns : the end of this range Args : optionaly allows the end to be set : using $loc->end($start) =cut sub end { my ($self, $value) = @_; $self->min_end($value) if( defined $value ); return $self->SUPER::end(); } =head2 strand Title : strand Usage : $strand = $loc->strand(); Function: get/set the strand of this range Returns : the strandidness (-1, 0, +1) Args : optionaly allows the strand to be set : using $loc->strand($strand) =cut sub strand { my $self = shift; if ( @_ ) { my $value = shift; if ( defined($value) ) { if ( $value eq '+' ) { $value = 1; } elsif ( $value eq '-' ) { $value = -1; } elsif ( $value eq '.' ) { $value = 0; } elsif ( $value != -1 && $value != 1 && $value != 0 ) { $self->throw("$value is not a valid strand info"); } $self->{'_strand'} = $value; } } # do not pretend the strand has been set if in fact it wasn't return $self->{'_strand'}; #return $self->{'_strand'} || 0; } =head2 flip_strand Title : flip_strand Usage : $location->flip_strand(); Function: Flip-flop a strand to the opposite Returns : None Args : None =cut sub flip_strand { my $self= shift; $self->strand($self->strand * -1); } =head2 seq_id Title : seq_id Usage : my $seqid = $location->seq_id(); Function: Get/Set seq_id that location refers to Returns : seq_id (a string) Args : [optional] seq_id value to set =cut sub seq_id { my ($self, $seqid) = @_; if( defined $seqid ) { $self->{'_seqid'} = $seqid; } return $self->{'_seqid'}; } =head2 length Title : length Usage : $len = $loc->length(); Function: get the length in the coordinate space this location spans Example : Returns : an integer Args : none =cut sub length { my ($self) = @_; return abs($self->end() - $self->start()) + 1; } =head2 min_start Title : min_start Usage : my $minstart = $location->min_start(); Function: Get minimum starting location of feature startpoint Returns : integer or undef if no minimum starting point. Args : none =cut sub min_start { my ($self,$value) = @_; if(defined($value)) { $self->{'_start'} = $value; } return $self->{'_start'}; } =head2 max_start Title : max_start Usage : my $maxstart = $location->max_start(); Function: Get maximum starting location of feature startpoint. In this implementation this is exactly the same as min_start(). Returns : integer or undef if no maximum starting point. Args : none =cut sub max_start { my ($self,@args) = @_; return $self->min_start(@args); } =head2 start_pos_type Title : start_pos_type Usage : my $start_pos_type = $location->start_pos_type(); Function: Get start position type (ie <,>, ^). In this implementation this will always be 'EXACT'. Returns : type of position coded as text ('BEFORE', 'AFTER', 'EXACT','WITHIN', 'BETWEEN') Args : none =cut sub start_pos_type { my($self) = @_; return 'EXACT'; } =head2 min_end Title : min_end Usage : my $minend = $location->min_end(); Function: Get minimum ending location of feature endpoint Returns : integer or undef if no minimum ending point. Args : none =cut sub min_end { my($self,$value) = @_; if(defined($value)) { $self->{'_end'} = $value; } return $self->{'_end'}; } =head2 max_end Title : max_end Usage : my $maxend = $location->max_end(); Function: Get maximum ending location of feature endpoint In this implementation this is exactly the same as min_end(). Returns : integer or undef if no maximum ending point. Args : none =cut sub max_end { my($self,@args) = @_; return $self->min_end(@args); } =head2 end_pos_type Title : end_pos_type Usage : my $end_pos_type = $location->end_pos_type(); Function: Get end position type (ie <,>, ^) In this implementation this will always be 'EXACT'. Returns : type of position coded as text ('BEFORE', 'AFTER', 'EXACT','WITHIN', 'BETWEEN') Args : none =cut sub end_pos_type { my($self) = @_; return 'EXACT'; } =head2 location_type Title : location_type Usage : my $location_type = $location->location_type(); Function: Get location type encoded as text Returns : string ('EXACT', 'WITHIN', 'IN-BETWEEN') Args : none =cut sub location_type { my ($self) = @_; return 'EXACT'; } =head2 is_remote Title : is_remote Usage : $self->is_remote($newval) Function: Getset for is_remote value Returns : value of is_remote Args : newvalue (optional) =cut sub is_remote { my $self = shift; if( @_ ) { my $value = shift; $self->{'is_remote'} = $value; } return $self->{'is_remote'}; } =head2 each_Location Title : each_Location Usage : @locations = $locObject->each_Location($order); Function: Conserved function call across Location:: modules - will return an array containing the component Location(s) in that object, regardless if the calling object is itself a single location or one containing sublocations. Returns : an array of Bio::LocationI implementing objects - for Simple locations, the return value is just itself. Args : =cut sub each_Location { my ($self) = @_; return ($self); } =head2 to_FTstring Title : to_FTstring Usage : my $locstr = $location->to_FTstring() Function: returns the FeatureTable string of this location Returns : string Args : none =cut sub to_FTstring { my($self) = @_; if( $self->start == $self->end ) { return $self->start; } my $str = $self->start . ".." . $self->end; if( $self->strand == -1 ) { $str = sprintf("complement(%s)", $str); } return $str; } =head2 valid_Location Title : valid_Location Usage : if ($location->valid_location) {...}; Function: boolean method to determine whether location is considered valid (has minimum requirements for Simple implementation) Returns : Boolean value: true if location is valid, false otherwise Args : none =cut sub valid_Location { my ($self) = @_; return 1 if $self->{'_start'} && $self->{'_end'}; return 0; } =head2 coordinate_policy Title : coordinate_policy Usage : $policy = $location->coordinate_policy(); $location->coordinate_policy($mypolicy); # set may not be possible Function: Get the coordinate computing policy employed by this object. See L for documentation about the policy object and its use. The interface *does not* require implementing classes to accept setting of a different policy. The implementation provided here does, however, allow to do so. Implementors of this interface are expected to initialize every new instance with a L object. The implementation provided here will return a default policy object if none has been set yet. To change this default policy object call this method as a class method with an appropriate argument. Note that in this case only subsequently created Location objects will be affected. Returns : A L implementing object. Args : On set, a L implementing object. See L for more information =cut sub coordinate_policy { my ($self, $policy) = @_; if(defined($policy)) { if(! $policy->isa('Bio::Location::CoordinatePolicyI')) { $self->throw("Object of class ".ref($policy)." does not implement". " Bio::Location::CoordinatePolicyI"); } if(ref($self)) { $self->{'_coordpolicy'} = $policy; } else { # called as class method $coord_policy = $policy; } } return (ref($self) && exists($self->{'_coordpolicy'}) ? $self->{'_coordpolicy'} : $coord_policy); } =head2 trunc Title : trunc Usage : $trunc_location = $location->trunc($start, $end, $relative_ori); Function: To truncate a location and keep annotations and features within the truncated segment intact. This might do things differently where the truncation splits the location in half. CAVEAT : As yet, this is an untested and unannounced method. Use with caution! Returns : A L object. Args : The start and end position for the trunction, and the relative orientation. =cut sub trunc { my ($self,$start,$end,$relative_ori) = @_; my $newstart = $self->start - $start+1; my $newend = $self->end - $start+1; my $newstrand = $relative_ori * $self->strand; my $out; if( $newstart < 1 || $newend > ($end-$start+1) ) { $out = Bio::Location::Atomic->new(); $out->start($self->start); $out->end($self->end); $out->strand($self->strand); $out->seq_id($self->seqid); $out->is_remote(1); } else { $out = Bio::Location::Atomic->new(); $out->start($newstart); $out->end($newend); $out->strand($newstrand); $out->seq_id(); } return $out; } 1;