# $Id: Contig.pm 16123 2009-09-17 12:57:27Z cjfields $ # # BioPerl module for Bio::Map::Contig # # Please direct questions and support issues to # # Cared for by Sendu Bala # # Copyright Gaurav Gupta # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code =head1 NAME Bio::Map::Contig - A MapI implementation handling the contigs of a Physical Map (such as FPC) =head1 SYNOPSIS # get the contig object of $contig from the Bio::Map::Physical my $ctgobj = $physical->get_contigobj($contig); # acquire all the markers that lie in this contig foreach my $marker ($ctgobj->each_markerid()) { print " +++$marker\n"; } # find the group of this contig print "Group: ",$ctgobj->group(),"\n"; # find the range of this contig print "RANGE: start:",$ctgobj->range()->start(),"\tend: ", $ctgobj->range()->end(),"\n"; # find the position of this contig in $group (chromosome) print "Position in Group $group"," = ",$ctgobj->position($group),"\n"; =head1 DESCRIPTION This is an implementation of Bio::Map::MapI. It handles the essential storage of name, species, type, and units as well as in memory representation of the elements of a map. Bio::Map::Contig has been tailored to work for FPC physical maps, but could probably be used for others as well (with the appropriate MapIO module). =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHOR - Gaurav Gupta Email gaurav@genome.arizona.edu =head1 CONTRIBUTORS Sendu Bala bix@sendu.me.uk =head1 PROJECT LEADERS Jamie Hatfield jamie@genome.arizona.edu Dr. Cari Soderlund cari@genome.arizona.edu =head1 PROJECT DESCRIPTION The project was done in Arizona Genomics Computational Laboratory (AGCoL) at University of Arizona. This work was funded by USDA-IFAFS grant #11180 titled "Web Resources for the Computation and Display of Physical Mapping Data". For more information on this project, please refer: http://www.genome.arizona.edu =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::Map::Contig; use vars qw($MAPCOUNT); use strict; # Object preamble - inherits from Bio::Root::Root use Bio::Range; use base qw(Bio::Map::SimpleMap); BEGIN { $MAPCOUNT = 1; } =head2 new Title : new Usage : my $clone = Bio::Map::Contig->new ( -name => $name, -chr_remark => $cremark, -user_remark => $uremark, -trace_remark => $tremark, -group => $group, -subgroup=> $subgroup, -anchor => $anchor, -markers => \%markers, -clones => \%clones, -position => $pos -range => Bio::Range->new(-start =>$s,-end=>$e), ); Function: Initialize a new Bio::Map::Contig object Most people will not use this directly but get Markers through L Returns : L object Args : ( -name => name string, -chr_remark => chr remark string, -user_remark => userremark string, -trace_remark => tremark string, -group => group string, -subgroup=> subgroup string, -anchor => boolean if this is anchored or not, -markers => hashref of contained markers, -clones => hashref of contained clones, -position => position -range => L =cut sub new { my ($class,@args) = @_; my $self = $class->SUPER::new(@args); my ($name,$cremark,$uremark,$tremark, $group,$subgroup, $anchor,$markers, $clones, $position,$range) = $self->_rearrange([qw(NAME CHR_REMARK USER_REMARK TRACE_REMARK GROUP SUBGROUP ANCHOR MARKERS CLONES POSITION RANGE)],@args); $self->name($name) if defined $name; $self->chr_remark($cremark) if defined $cremark; $self->user_remark($uremark) if defined $uremark; $self->trace_remark($tremark) if defined $tremark; $self->group($group) if defined $group; $self->subgroup($group) if defined $subgroup; $self->anchor($anchor) if defined $anchor; $self->set_markers($markers) if defined $markers; $self->set_clones($clones) if defined $clones; $self->range($range) if defined $range; $self->position($position) if defined $position; return $self; } =head2 Modifier methods All methods present in L are implemented by this class. Most of the methods are inherited from SimpleMap. The following methods have been modified to reflect the needs of physical maps. =head2 chr_remark Title : chr_remark Usage : my $chrremark = $contigobj->chr_remark(); Function: Get/set the group remark for this contig Returns : scalar representing the current group_remark of this contig Args : none to get, OR string to set =cut sub chr_remark { my ($self) = shift; $self->{'_cremark'} = shift if @_; return defined $self->{'_cremark'} ? $self->{'_cremark'} : ''; } =head2 user_remark Title : user_remark Usage : my $userremark = $contigobj->user_remark(); Function: Get/set the user remark for this contig Returns : scalar representing the current user_remark of this contig Args : none to get, OR string to set =cut sub user_remark { my ($self) = shift; $self->{'_uremark'} = shift if @_; return defined $self->{'_uremark'} ? $self->{'_uremark'} : ''; } =head2 trace_remark Title : trace_remark Usage : my $traceremark = $contigobj->trace_remark(); Function: Get/set the trace remark for this contig Returns : scalar representing the current trace_remark of this contig Args : none to get, OR string to set =cut sub trace_remark { my ($self) = shift; $self->{'_tremark'} = shift if @_; return defined $self->{'_tremark'} ? $self->{'_tremark'} : ''; } =head2 range Title : range Usage : my $range = $contigobj->range(); Function: Get/set the range for this Contig Returns : Bio::Range representing the current range of this contig, start and end of the contig can be thus found using: my $start = $contigobj->range()->start(); my $end = $contigobj->range()->end(); Args : none to get, OR Bio::Range to set =cut sub range { my ($self) = shift; return $self->{'_range'} = shift if @_; return $self->{'_range'}; } =head2 position Title : position Usage : $ctgpos = $contigobj->position(); Function: Get/set the position of the contig in the group Returns : scalar representing the position of the contig in the group Args : none to get, OR string to set =cut sub position { my ($self) = shift; $self->{'_position'} = shift if @_; return $self->{'_position'} || 0; } =head2 anchor Title : anchor Usage : $ctganchor = $contig->anchor(); Function: Get/set the anchor value for this Contig (True | False) Returns : scalar representing the anchor (1 | 0) for this contig Args : none to get, OR string to set =cut sub anchor { my ($self) = shift; return $self->{'_anchor'} = shift if @_; return $self->{'_anchor'}; } =head2 group Title : group Usage : $groupno = $contigobj->group(); Function: Get/set the group number for this contig. This is a generic term, used for Linkage-Groups as well as for Chromosomes. Returns : scalar representing the group number of this contig Args : none =cut sub group { my ($self) = shift; $self->{'_group'} = shift if @_; return $self->{'_group'} || 0; } =head2 subgroup Title : subgroup Usage : $subgroup = $contig->subgroup(); Function: Get/set the subgroup for this contig. This is a generic term: subgroup here could represent subgroup of a Chromosome or of a Linkage Group. The user must take care of which subgroup he/she is querying for. Returns : A scalar representing the subgroup of this contig Args : none =cut sub subgroup { my ($self) = @_; return $self->{'_subgroup'} = shift if @_; return $self->{'_subgroup'} || 0; } =head2 each_cloneid Title : each_cloneid Usage : my @clones = $map->each_cloneid(); Function: retrieves all the clone ids in a map unordered Returns : list of strings (ids) Args : none *** This only supplies the ids set with the set_clones method *** *** It has nothing to do with actual Bio::Map::MappableI objects *** =cut sub each_cloneid { my ($self) = @_; return $self->_each_element('clones'); } =head2 each_markerid Title : each_markerid Usage : my @markers = $map->each_markerid(); Function: retrieves all the marker ids in a map unordered Returns : list of strings (ids) Args : none *** This only supplies the ids set with the set_markers method *** *** It has nothing to do with actual Bio::Map::MarkerI objects *** =cut sub each_markerid { my ($self) = @_; return $self->_each_element('markers'); } sub _each_element { my ($self, $type) = @_; $type = 'clones' if (!defined($type)); $type = lc("_$type"); return keys %{$self->{$type} || {}}; } =head2 set_clones Title : set_clones Usage : $marker->set_clones(\%clones) Function: Set the clones hashref Returns : None Args : Hashref of clone ids *** This only sets a hash of ids *** *** It has nothing to do with actual Bio::Map::MappableI objects *** =cut sub set_clones { my ($self,$clones) = @_; if( defined $clones && ref($clones) =~ /HASH/ ) { $self->{'_clones'} = $clones; } } =head2 set_markers Title : markers Usage : $obj->set_markers($newval) Function: Set list of Markers (hashref) Returns : None Args : Hashref of marker ids *** This only sets a hash of ids *** *** It has nothing to do with actual Bio::Map::MarkerI objects *** =cut sub set_markers { my ($self,$markers) = @_; if( defined $markers && ref($markers) =~ /HASH/ ) { $self->{'_markers'} = $markers; } } 1;