# $Id: FPCMarker.pm 16123 2009-09-17 12:57:27Z cjfields $ # # BioPerl module for Bio::Map::fpcmarker # # Please direct questions and support issues to # # Cared for by Gaurav Gupta # # Copyright Gaurav Gupta # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code =head1 NAME Bio::Map::FPCMarker - An central map object representing a marker =head1 SYNOPSIS # get the marker object of $marker from the Bio::Map::FPCMarker my $markerobj = $physical->get_markerobj($marker); # acquire all the clones that hit this marker foreach my $clone ($markerobj->each_cloneid()) { print " +++$clone\n"; } # find the position of this marker in $contig print "Position in contig $contig"," = ",$markerobj->position($contig), "\n"; # find the group of the marker print "Group : ",$markerobj->group(); See L and L for more information. =head1 DESCRIPTION This object handles the notion of a marker. This object is intended to be used by a map parser like fpc.pm. =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHOR - Gaurav Gupta Email gaurav@genome.arizona.edu =head1 CONTRIBUTORS Sendu Bala bix@sendu.me.uk =head1 PROJECT LEADERS Jamie Hatfield jamie@genome.arizona.edu Dr. Cari Soderlund cari@genome.arizona.edu =head1 PROJECT DESCRIPTION The project was done in Arizona Genomics Computational Laboratory (AGCoL) at University of Arizona. This work was funded by USDA-IFAFS grant #11180 titled "Web Resources for the Computation and Display of Physical Mapping Data". For more information on this project, please refer: http://www.genome.arizona.edu =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::Map::FPCMarker; use strict; use Bio::Map::Position; use Time::Local; use base qw(Bio::Root::Root Bio::Map::MappableI); =head2 new Title : new Usage : my $clone = Bio::Map::FPCMarker->new ( -name => $marker, -type => $type, -global => $global, -frame => $frame, -group => $group, -subgroup=> $subgroup, -anchor => $anchor, -clones => \%clones, -contigs => \%contigs, -position => \%markerpos, -remark => $remark ); Function: Initialize a new Bio::Map::FPCMarker object Most people will not use this directly but get Markers through L Returns : L object Args : -name => marker name string, -type => type string, -global => global position for marker, -frame => boolean if marker is framework or placement, -group => group number for marker, -subgroup => subgroup number of marker, -anchor => boolean if marker is anchored, -clones => all the clone elements in map (hashref), -contigs => all the contig elements (hasref), -position => mapping of marker names to map position (hasref), -remark => remarks, separated by newlines =cut sub new { my ($class,@args) = @_; my $self= $class->SUPER::new(@args); my ($name,$type,$global,$frame,$group, $subgroup, $anchor, $clones,$contigs, $positions, $remark) = $self->_rearrange([qw(NAME TYPE GLOBAL FRAME GROUP SUBGROUP ANCHOR CLONES CONTIGS POSITIONS REMARK)],@args); $self->name($name) if defined $name; $self->type($type) if defined $type; $self->global($global) if defined $global; $self->group($group) if defined $group; $self->subgroup($group) if defined $subgroup; $self->anchor($anchor) if defined $anchor; $self->remark($remark) if defined $remark; $self->set_clones($clones) if defined $clones; $self->set_contigs($contigs) if defined $contigs; $self->set_positions($positions) if defined $positions; return $self; } =head1 Access Methods These methods let you get and set the member variables =head2 name Title : name Usage : my $name = $markerobj->name(); Function: Get/set the name for this marker Returns : scalar representing the current name of this marker Args : none to get, OR string to set =cut sub name { my ($self) = shift; return $self->{'_name'} = shift if @_; return $self->{'_name'}; } =head2 type Title : type Usage : my $type = $markerobj->type(); Function: Get/set the type for this marker Returns : scalar representing the current type of this marker Args : none to get, OR string to set =cut sub type { my ($self) = shift; return $self->{'_type'} = shift if @_; return $self->{'_type'}; } =head2 global Title : global Usage : my $type = $markerobj->global(); Function: Get/set the global position for this marker Returns : scalar representing the current global position of this marker Args : none to get, OR string to set =cut sub global { my ($self) = shift; return $self->{'_global'} = shift if @_; return $self->{'_global'}; } =head2 anchor Title : anchor Usage : my $anchor = $markerobj->anchor(); Function: indicate if the Marker is anchored or not (True | False) Returns : scalar representing the anchor (1 | 0) for this marker Args : none to get, OR 1|0 to set =cut sub anchor { my ($self) = shift; return $self->{'_anchor'} = shift if @_; return $self->{'_anchor'}; } =head2 framework Title : framework Usage : $frame = $markerobj->framework(); Function: indicate if the Marker is framework or placement (1 | 0) Returns : scalar representing if the marker is framework (1 if framework, 0 if placement) Args : none to get, OR 1|0 to set =cut sub framework { my ($self) = shift; return $self->{'_frame'} = shift if @_; return $self->{'_frame'}; } =head2 group Title : group Usage : $grpno = $markerobj->group(); Function: Get/set the group number for this marker. This is a generic term, used for Linkage-Groups as well as for Chromosomes. Returns : scalar representing the group number of this marker Args : none to get, OR string to set =cut sub group { my ($self) = shift; $self->{'_group'} = shift if @_; return $self->{'_group'} || 0; } =head2 subgroup Title : subgroup Usage : $subgroup = $marker->subgroup(); Function: Get/set the subgroup for this marker. This is a generic term: subgroup here could represent subgroup of a Chromosome or of a Linkage Group. The user must take care of which subgroup he/she is querying for. Returns : scalar representing the subgroup of this marker Args : none to get, OR string to set =cut sub subgroup { my ($self) = shift; $self->{'_subgroup'} = shift if @_; return $self->{'_subgroup'} || 0; } =head2 position Title : position Usage : $markerpos = $markerobj->position($ctg); Function: get the position of the marker in the contig Returns : scalar representing the position of the markernumber of the contig Args : $ctg is necessary to look for the position of the marker in that contig. *** This has nothing to do with an actual Bio::Map::PositionI object *** =cut sub position { my ($self,$ctg) = @_; return 0 unless defined $ctg; return 0 unless( defined $self->{'_position'} && defined $self->{'_position'}{$ctg}); return $self->{'_position'}{$ctg}; } =head2 remark Title : remark Usage : $markerremark = $markerobj->remark(); Function: get the remarks for this marker Returns : scalar of newline-separated markers Args : none =cut sub remark { my ($self) = shift; return $self->{'_remark'} = shift if @_; return $self->{'_remark'}; } =head2 each_cloneid Title : each_cloneid Usage : my @clones = $map->each_cloneid(); Function: retrieves all the clone ids in a map unordered Returns : list of strings (ids) Args : none *** This only supplies the ids set with the set_clones method *** *** It has nothing to do with actual Bio::Map::MappableI objects *** =cut sub each_cloneid { my ($self) = @_; return $self->_each_element('clones'); } =head2 each_contigid Title : each_contigid Usage : my @contigs = $map->each_contigid(); Function: retrieves all the contig ids in a map unordered Returns : list of strings (ids) Args : none *** This only supplies the ids set with the set_contigs method *** *** It has nothing to do with actual Bio::Map::MapI objects *** =cut sub each_contigid { my ($self) = @_; return $self->_each_element('contigs'); } sub _each_element{ my ($self, $type) = @_; $type = 'clones' unless defined $type; $type = lc("_$type"); return keys %{$self->{$type} || {}}; } =head2 set_clones Title : set_clones Usage : $marker->set_clones(\%clones) Function: Set the clone ids hashref Returns : None Args : Hashref of clone ids *** This only sets a hash of ids *** *** It has nothing to do with actual Bio::Map::MappableI objects *** =cut sub set_clones{ my ($self,$clones) = @_; if( defined $clones && ref($clones) =~ /HASH/ ) { $self->{'_clones'} = $clones; } } =head2 set_contigs Title : set_contigs Usage : $marker->set_contigs(\%contigs) Function: Set the contig ids hashref Returns : None Args : Hashref of contig ids *** This only sets a hash of ids *** *** It has nothing to do with actual Bio::Map::MapI objects *** =cut sub set_contigs{ my ($self,$contigs) = @_; if( defined $contigs && ref($contigs) =~ /HASH/ ) { $self->{'_contigs'} = $contigs; } } =head2 set_positions Title : set_positions Usage : $marker->set_positions(\%markerpos) Function: Set the positions hashref Returns : None Args : Hashref of marker positions *** This only sets a hash of numbers *** *** It has nothing to do with actual Bio::Map::PositionI objects *** =cut sub set_positions{ my ($self,$pos) = @_; if( defined $pos && ref($pos) =~ /HASH/ ) { $self->{'_positions'} = $pos; } } 1;