# BioPerl module for Bio::Map::LinkageMap # # Please direct questions and support issues to # # Cared for by Sendu Bala # # Copyright Chad Matsalla # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code =head1 NAME Bio::Map::LinkageMap - A representation of a genetic linkage map. =head1 SYNOPSIS use Bio::Map::LinkageMap; # create a new map my $map = Bio::Map::LinkageMap->new(-name => 'Chads Superterriffic Map', -type => 'Linkage', -units=> 'cM'); # create the location of a marker for that map my $position = Bio::Map::LinkagePosition->new( -positions => 1, -distance => "22.3"); # create a marker and place it at that position my $marker = Bio::Map::Marker::Microsatellite->new( -name => 'SuuuperMarker', -position => $position); # place that marker on that map $map->add_element($marker); # done! =head1 DESCRIPTION This object describes the basic functionality of a genetic linkage map in Bioperl. Each 'position' can have one or more markers that map some number of units from the markers at the previous position. =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHOR - Chad Matsalla Email bioinformatics1@dieselwurks.com =head1 CONTRIBUTORS Lincoln Stein lstein@cshl.org Heikki Lehvaslaiho heikki-at-bioperl-dot-org Jason Stajich jason@bioperl.org Sendu Bala bix@sendu.me.uk =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::Map::LinkageMap; use strict; use base qw(Bio::Map::SimpleMap); =head2 new Title : new Usage : my $linkage_map = Bio::Map::LinkageMap->new(); Function: Builds a new Bio::Map::LinkageMap object Returns : Bio::Map::LinkageMap Args : -name => the name of the map (string) [optional] -type => the type of this map (string, defaults to Linkage) [optional] -species => species for this map (Bio::Species) [optional] -units => the map units (string, defaults to cM) [optional] -elements=> elements to initialize with (arrayref of Bio::Map::MappableI objects) [optional] -uid => Unique ID of this map =cut =head2 length Title : length Usage : my $length = $map->length(); Function: Retrieves the length of the map. In the case of a LinkageMap, the length is the sum of all marker distances. Returns : An integer representing the length of this LinkageMap. Will return 0 if length is not calculateable Args : None. =cut sub length { my ($self) = @_; $self->throw("Not yet implemented correctly"); my $total_distance; foreach my $element (@{$self->get_elements}) { #*** there is no such method ->each_position_value! $total_distance += ($element->position->each_position_value($self))[0]; } return $total_distance; } =head2 add_element($marker) Title : add_element($marker) Usage : $map->add_element($marker) Function: Add a Bio::Map::MappableI object to the Map Returns : none Args : Bio::Map::MappableI object Notes : It is strongly recommended that you use a Bio::Map::LinkagePosition as the position in any Bio::Map::Mappable that you create to place on this map. Using some other Bio::Map::Position might work but might be unpredictable. N.B. I've added Bio::Map::OrderedPosition which should achieve similar things from LinkagePosition and will work for RH markers too. =cut #*** what is this? what calls it? note that it seems to be private sub _add_element_will_be_deleted { my ($self,$marker) = @_; my $o_position = $marker->position(); $self->debug( "marker position is ". $marker->position()); # print("add_element: \$o_position is $o_position\n"); # print("add_element: \$marker is $marker\n"); my $position; unless ( $o_position->isa('Bio::Map::LinkagePosition') || $o_position->isa('Bio::Map::OrderedPosition') ) { $self->warn("You really should use a Linkage Position for this object. This insures that there is only one position. Trying anyway..."); my @p = ( $o_position->each_position_value($self)); $position = shift @p; if( ! defined $position ) { $self->throw("This marker ($marker) does not have a position in this map ($self)"); } } else { $position = $o_position->order; } if ($self->{'_elements'}[$position]) { $self->warn("Replacing the marker in position $position because in a linkage map the position is a key."); } $self->{'_elements'}[$position] = $marker; } 1;