# $Id: Marker.pm 16123 2009-09-17 12:57:27Z cjfields $ # # BioPerl module for Bio::Map::Marker # # Please direct questions and support issues to # # Cared for by Sendu Bala # # Copyright Chad Matsalla # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code =head1 NAME Bio::Map::Marker - An central map object representing a generic marker that can have multiple location in several maps. =head1 SYNOPSIS # get map objects somehow # a marker with complex localisation $o_usat = Bio::Map::Marker->new(-name=>'Chad Super Marker 2', -positions => [ [$map1, $position1], [$map1, $position2] ] ); # The markers deal with Bio::Map::Position objects which can also # be explicitely created and passed on to markers as an array ref: $o_usat2 = Bio::Map::Marker->new(-name=>'Chad Super Marker 3', -positions => [ $pos1, $pos2 ] ); # a marker with unique position in a map $marker1 = Bio::Map::Marker->new(-name=>'hypervariable1', -map => $map1, -position => 100 ); # an other way of creating a marker with unique position in a map: $marker2 = Bio::Map::Marker->new(-name=>'hypervariable2'); $map1->add_element($marker2); $marker2->position(100); # position method is a short cut for get/setting unique positions # which overwrites previous values # to place a marker to other maps or to have multiple positions # for a map within the same map use add_position() $marker2->add_position(200); # new position in the same map $marker2->add_position($map2,200); # new map # setting a map() in a marker or adding a marker into a map are # identical mathods. Both set the bidirectional connection which is # used by the marker to remember its latest, default map. # Regardes of how marker positions are created, they are stored and # returned as Bio::Map::PositionI objects: # unique position print $marker1->position->value, "\n"; # several positions foreach $pos ($marker2->each_position($map1)) { print $pos->value, "\n"; } See L and L for more information. =head1 DESCRIPTION A Marker is a Bio::Map::Mappable with some properties particular to markers. It also offers a number of convienience methods to make dealing with map elements easier. =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHOR - Chad Matsalla Email bioinformatics1@dieselwurks.com =head1 CONTRIBUTORS Heikki Lehvaslaiho heikki-at-bioperl-dot-org Lincoln Stein lstein@cshl.org Jason Stajich jason@bioperl.org Sendu Bala bix@sendu.me.uk =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::Map::Marker; use strict; use Bio::Map::Position; use base qw(Bio::Map::Mappable Bio::Map::MarkerI); =head2 new Title : new Usage : my $marker = Bio::Map::Marker->new( -name => 'Whizzy marker', -position => $position); Function: Builds a new Bio::Map::Marker object Returns : Bio::Map::Marker Args : -name => name of this microsatellite [optional], string,default 'Unknown' -default_map => the default map for this marker, a Bio::Map::MapI -position => map position for this marker, a Bio::Map::PositionI -positions => array ref of Bio::Map::PositionI objects position and positions can also take as values anything the corresponding methods can take =cut sub new { my ($class, @args) = @_; my $self = $class->SUPER::new(@args); bless($self, ref $class || $class); my ($name, $default_map, $map, $position, $positions) = $self->_rearrange([qw(NAME DEFAULT_MAP MAP POSITION POSITIONS )], @args); if ($name) { $self->name($name); } else {$self->name('Unnamed marker'); } $map && $self->default_map($map); $default_map && $self->default_map($default_map); $position && $self->position($position); $positions && $self->positions($positions); return $self; } =head2 default_map Title : default_map Usage : my $map = $marker->default_map(); Function: Get/Set the default map for the marker. Returns : L Args : [optional] new L =cut sub default_map { my ($self, $map) = @_; if (defined $map) { $self->thow("This is [$map], not Bio::Map::MapI object") unless $map->isa('Bio::Map::MapI'); $self->{'_default_map'} = $map; } return $self->{'_default_map'} || return; } =head2 map Title : map Function: This is a synonym of the default_map() method *** does not actually add this marker to the map! *** Status : deprecated, will be removed in next version =cut *map = \&default_map; =head2 get_position_object Title : get_position_class Usage : my $position = $marker->get_position_object(); Function: To get an object of the default Position class for this Marker. Subclasses should redefine this method. The Position returned needs to be a L with -element set to self. Returns : L Args : none for an 'empty' PositionI object, optionally Bio::Map::MapI and value string to set the Position's -map and -value attributes. =cut sub get_position_object { my ($self, $map, $value) = @_; $map ||= $self->default_map; if ($value) { $self->throw("Value better be scalar, not [$value]") unless ref($value) eq ''; } my $pos = Bio::Map::Position->new(); $pos->map($map) if $map; $pos->value($value) if defined($value); $pos->element($self); return $pos; } =head2 position Title : position Usage : my $position = $mappable->position(); $mappable->position($position); Function: Get/Set the Position of this Marker (where it is on which map), purging all other positions before setting. Returns : L Args : Bio::Map::PositionI OR Bio::Map::MapI AND scalar OR scalar, but only if the marker has a default map =cut sub position { my ($self, $pos, $pos_actual) = @_; if ($pos) { $self->purge_positions; $self->add_position($pos, $pos_actual); } my @positions = $self->each_position; $self->warn('This marker has more than one Position, returning the most recently added') if scalar @positions > 1; return pop(@positions); } =head2 add_position Title : add_position Usage : $marker->add_position($position); Function: Add a Position to this marker Returns : n/a Args : Bio::Map::PositionI OR Bio::Map::MapI AND scalar OR scalar, but only if the marker has a default map =cut sub add_position { my ($self, $pos, $pos_actual) = @_; $self->throw("Must give a Position") unless defined $pos; my $map = $self->default_map; my $pos_map; if (ref($pos)) { if (ref($pos) eq 'ARRAY') { ($pos, $pos_actual) = @{$pos}; unless ($pos && $pos_actual && ref($pos)) { $self->throw("Supplied an array ref but did not contain two values, the first an object"); } } if ($pos->isa('Bio::Map::PositionI')) { $pos_map = $pos->map; $self->default_map($pos_map) unless $map; $map = $pos_map if $pos_map; } elsif ($pos->isa('Bio::Map::MapI')) { $self->default_map($pos) unless $map; $map = $pos; $pos = $pos_actual; } else { $self->throw("This is [$pos], not a Bio::Map::PositionI or Bio::Map::MapI object"); } } $self->throw("You need to give a marker a default map before you can set positions without explicit map!" ) unless $map; if (ref($pos) && $pos->isa('Bio::Map::PositionI')) { $pos->map($map) unless $pos_map; $self->SUPER::add_position($pos); } else { $self->get_position_object($map, $pos); # adds position to us } } =head2 positions Title : positions Usage : $marker->positions([$pos1, $pos2, $pos3]); Function: Add multiple Bio::Map::PositionI to this marker Returns : n/a Args : array ref of $map/value tuples or array ref of Bio::Map::PositionI =cut sub positions { my ($self, $args_ref) = @_; foreach my $arg (@{$args_ref}) { if (ref($arg) eq 'ARRAY') { $self->add_position(@{$arg}); } else { $self->add_position($arg); } } } =head2 in_map Title : in_map Usage : if ( $marker->in_map($map) ) {} Function: Tests if this marker is found on a specific map Returns : boolean Args : a map unique id OR Bio::Map::MapI =cut sub in_map { my ($self, $query) = @_; $self->throw("Must supply an argument") unless defined($query); if (ref($query) eq '') { foreach my $map ($self->known_maps) { my $uid = $map->unique_id; if ($uid) { ($uid eq $query) && return 1; } } } else { return $self->SUPER::in_map($query); } return 0; } 1;