# $Id: PositionI.pm 16123 2009-09-17 12:57:27Z cjfields $ # # BioPerl module for Bio::Map::PositionI # # Please direct questions and support issues to # # Cared for by Sendu Bala # # Copyright Jason Stajich # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code =head1 NAME Bio::Map::PositionI - Abstracts the notion of a position having a value in the context of a marker and a Map =head1 SYNOPSIS # do not use this module directly # See Bio::Map::Position for an example of # implementation. =head1 DESCRIPTION This object stores one of the postions that a mappable object (e.g. Marker) may have in a map. Positions can have non-numeric values or other methods to store the locations, so they have a method numeric() which does the conversion. numeric() returns the position in a form that can be compared between other positions of the same type. It is not necessarily a value suitable for sorting positions (it may be the distance from the previous position); for that purpose the result of sortable() should be used. A 'position', in addition to being a single point, can also be an area and so can be imagined as a range and compared with other positions on the basis of overlap, intersection etc. =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHOR - Jason Stajich Email jason-at-bioperl.org =head1 CONTRIBUTORS Lincoln Stein, lstein-at-cshl.org Heikki Lehvaslaiho, heikki-at-bioperl-dot-org Sendu Bala, bix@sendu.me.uk =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::Map::PositionI; use strict; use Bio::Map::PositionHandler; use Bio::Map::Mappable; use Scalar::Util qw(looks_like_number); use base qw(Bio::Map::EntityI Bio::RangeI); =head2 EntityI methods These are fundamental to coordination of Positions and other entities, so are implemented at the interface level =cut =head2 get_position_handler Title : get_position_handler Usage : my $position_handler = $entity->get_position_handler(); Function: Gets a PositionHandlerI that $entity is registered with. Returns : Bio::Map::PositionHandlerI object Args : none =cut sub get_position_handler { my $self = shift; unless (defined $self->{_eh}) { my $ph = Bio::Map::PositionHandler->new(-self => $self); $self->{_eh} = $ph; $ph->register; } return $self->{_eh}; } =head2 PositionHandlerI-related methods These are fundamental to coordination of Positions and other entities, so are implemented at the interface level =cut =head2 map Title : map Usage : my $map = $position->map(); $position->map($map); Function: Get/Set the map the position is in. Returns : L Args : none to get new L to set =cut sub map { my ($self, $map) = @_; return $self->get_position_handler->map($map); } =head2 element Title : element Usage : my $element = $position->element(); $position->element($element); Function: Get/Set the element the position is for. Returns : L Args : none to get new L to set =cut sub element { my ($self, $element) = @_; return $self->get_position_handler->element($element); } =head2 marker Title : marker Function: This is a synonym of the element() method Status : deprecated, will be removed in the next version =cut *marker = \&element; =head2 PositionI-specific methods =cut =head2 value Title : value Usage : my $pos = $position->value(); Function: Get/Set the value for this position Returns : scalar, value Args : [optional] new value to set =cut sub value { my $self = shift; $self->throw_not_implemented(); } =head2 numeric Title : numeric Usage : my $num = $position->numeric; Function: Read-only method that is guaranteed to return a numeric representation of the start of this position. Returns : scalar numeric Args : none to get the co-ordinate normally (see absolute() method), OR Bio::Map::RelativeI to get the co-ordinate converted to be relative to what this Relative describes. =cut sub numeric { my $self = shift; $self->throw_not_implemented(); } =head2 sortable Title : sortable Usage : my $num = $position->sortable(); Function: Read-only method that is guaranteed to return a value suitable for correctly sorting this kind of position amongst other positions of the same kind on the same map. Note that sorting different kinds of position together is unlikely to give sane results. Returns : numeric Args : none =cut sub sortable { my $self = shift; $self->throw_not_implemented(); } =head2 relative Title : relative Usage : my $relative = $position->relative(); $position->relative($relative); Function: Get/set the thing this Position's coordinates (numerical(), start(), end()) are relative to, as described by a Relative object. Returns : Bio::Map::RelativeI (default is one describing "relative to the start of the Position's map") Args : none to get, OR Bio::Map::RelativeI to set =cut sub relative { my $self = shift; $self->throw_not_implemented(); } =head2 absolute Title : absolute Usage : my $absolute = $position->absolute(); $position->absolute($absolute); Function: Get/set how this Position's co-ordinates (numerical(), start(), end()) are reported. When absolute is off, co-ordinates are relative to the thing described by relative(). Ie. the value returned by start() will be the same as the value you set start() to. When absolute is on, co-ordinates are converted to be relative to the start of the map. So if relative() currently points to a Relative object describing "relative to another position which is 100 bp from the start of the map", this Position's start() had been set to 50 and absolute() returns 1, $position->start() will return 150. If absolute() returns 0 in the same situation, $position->start() would return 50. Returns : boolean (default 0) Args : none to get, OR boolean to set =cut sub absolute { my $self = shift; $self->throw_not_implemented(); } =head2 RangeI-based methods =cut =head2 start Title : start Usage : my $start = $position->start(); $position->start($start); Function: Get/set the start co-ordinate of this position. Returns : the start of this position Args : scalar numeric to set, OR none to get the co-ordinate normally (see absolute() method), OR Bio::Map::RelativeI to get the co-ordinate converted to be relative to what this Relative describes. =cut =head2 end Title : end Usage : my $end = $position->end(); $position->end($end); Function: Get/set the end co-ordinate of this position. Returns : the end of this position Args : scalar numeric to set, OR none to get the co-ordinate normally (see absolute() method), OR Bio::Map::RelativeI to get the co-ordinate converted to be relative to what this Relative describes. =cut =head2 length Title : length Usage : $length = $position->length(); Function: Get the length of this position. Returns : the length of this position Args : none =cut =head2 strand Title : strand Usage : $strand = $position->strand(); Function: Get the strand of this position; it is always 1 since maps to not have strands. Returns : 1 Args : none =cut sub strand { return 1; } =head2 toString Title : toString Usage : print $position->toString(), "\n"; Function: stringifies this range Returns : a string representation of the range of this Position Args : optional Bio::Map::RelativeI to have the co-ordinates reported relative to the thing described by that Relative =cut sub toString { my $self = shift; $self->throw_not_implemented(); } =head1 RangeI-related methods These methods work by considering only the values of start() and end(), as modified by considering every such co-ordinate relative to the start of the map (ie. absolute(1) is set temporarily during the calculation), or any supplied Relative. For the boolean methods, when the comparison Position is on the same map as the calling Position, there is no point supplying a Relative since the answer will be the same as without. Relative is most useful when comparing Positions on different maps and you have a Relative that describes some special place on each map like 'the start of the gene', where the actual start of the gene relative to the start of the map is different for each map. The methods do not consider maps during their calculations - things on different maps can overlap/contain/intersect/etc. each other. The geometrical methods (intersect, union etc.) do things to the geometry of ranges, and return Bio::Map::PositionI compliant objects or triplets (start, stop, strand) from which new positions could be built. When a PositionI is made it will have a map transferred to it if all the arguments shared the same map. If a Relative was supplied the result will have that same Relative. Note that the strand-testing args are there for compatability with the RangeI interface. They have no meaning when only using PositionI objects since maps do not have strands. Typically you will just set the argument to undef if you want to supply the argument after it. =head2 equals Title : equals Usage : if ($p1->equals($p2)) {...} Function: Test whether $p1 has the same start, end, length as $p2. Returns : true if they are describing the same position (regardless of map) Args : arg #1 = a Bio::RangeI (eg. a Bio::Map::Position) to compare this one to (mandatory) arg #2 = optional strand-testing arg ('strong', 'weak', 'ignore') arg #3 = optional Bio::Map::RelativeI to ask if the Positions equal in terms of their relative position to the thing described by that Relative =cut sub equals { # overriding the RangeI implementation so we can handle Relative my ($self, $other, $so, $rel) = @_; my ($own_start, $own_end) = $self->_pre_rangei($self, $rel); my ($other_start, $other_end) = $self->_pre_rangei($other, $rel); return ($self->_testStrand($other, $so) and $own_start == $other_start and $own_end == $other_end); } =head2 less_than Title : less_than Usage : if ($position->less_than($other_position)) {...} Function: Ask if this Position ends before another starts. Returns : boolean Args : arg #1 = a Bio::RangeI (eg. a Bio::Map::Position) to compare this one to (mandatory) arg #2 = optional Bio::Map::RelativeI to ask if the Position is less in terms of their relative position to the thing described by that Relative =cut sub less_than { my ($self, $other, $rel) = @_; my ($own_start, $own_end) = $self->_pre_rangei($self, $rel); my ($other_start, $other_end) = $self->_pre_rangei($other, $rel); return $own_end < $other_start; } =head2 greater_than Title : greater_than Usage : if ($position->greater_than($other_position)) {...} Function: Ask if this Position starts after another ends. Returns : boolean Args : arg #1 = a Bio::RangeI (eg. a Bio::Map::Position) to compare this one to (mandatory) arg #2 = optional Bio::Map::RelativeI to ask if the Position is greater in terms of their relative position to the thing described by that Relative =cut sub greater_than { my ($self, $other, $rel) = @_; my ($own_start, $own_end) = $self->_pre_rangei($self, $rel); my ($other_start, $other_end) = $self->_pre_rangei($other, $rel); return $own_start > $other_end; } =head2 overlaps Title : overlaps Usage : if ($p1->overlaps($p2)) {...} Function: Tests if $p1 overlaps $p2. Returns : True if the positions overlap (regardless of map), false otherwise Args : arg #1 = a Bio::RangeI (eg. a Bio::Map::Position) to compare this one to (mandatory) arg #2 = optional strand-testing arg ('strong', 'weak', 'ignore') arg #3 = optional Bio::Map::RelativeI to ask if the Positions overlap in terms of their relative position to the thing described by that Relative arg #4 = optional minimum percentage length of the overlap before reporting an overlap exists (default 0) =cut sub overlaps { # overriding the RangeI implementation so we can handle Relative my ($self, $other, $so, $rel, $min_percent) = @_; $min_percent ||= 0; my ($own_min, $other_min) = (0, 0); if ($min_percent > 0) { $own_min = (($self->length / 100) * $min_percent) - 1; $other_min = (($other->length / 100) * $min_percent) - 1; } my ($own_start, $own_end) = $self->_pre_rangei($self, $rel); my ($other_start, $other_end) = $self->_pre_rangei($other, $rel); return ($self->_testStrand($other, $so) and not (($own_start + $own_min > $other_end or $own_end - $own_min < $other_start) || ($own_start > $other_end - $other_min or $own_end < $other_start + $other_min))); } =head2 contains Title : contains Usage : if ($p1->contains($p2)) {...} Function: Tests whether $p1 totally contains $p2. Returns : true if the argument is totally contained within this position (regardless of map), false otherwise Args : arg #1 = a Bio::RangeI (eg. a Bio::Map::Position) to compare this one to, or scalar number (mandatory) arg #2 = optional strand-testing arg ('strong', 'weak', 'ignore') arg #3 = optional Bio::Map::RelativeI to ask if the Position is contained in terms of their relative position to the thing described by that Relative =cut sub contains { # overriding the RangeI implementation so we can handle Relative my ($self, $other, $so, $rel) = @_; my ($own_start, $own_end) = $self->_pre_rangei($self, $rel); my ($other_start, $other_end) = $self->_pre_rangei($other, $rel); return ($self->_testStrand($other, $so) and $other_start >= $own_start and $other_end <= $own_end); } =head2 intersection Title : intersection Usage : ($start, $stop, $strand) = $p1->intersection($p2) ($start, $stop, $strand) = Bio::Map::Position->intersection(\@positions); $mappable = $p1->intersection($p2, undef, $relative); $mappable = Bio::Map::Position->intersection(\@positions); Function: gives the range that is contained by all ranges Returns : undef if they do not overlap, OR Bio::Map::Mappable object who's positions are the cross-map-calculated intersection of the input positions on all the maps that the input positions belong to, OR, in list context, a three element array (start, end, strand) Args : arg #1 = [REQUIRED] a Bio::RangeI (eg. a Bio::Map::Position) to compare this one to, or an array ref of Bio::RangeI arg #2 = optional strand-testing arg ('strong', 'weak', 'ignore') arg #3 = optional Bio::Map::RelativeI to ask how the Positions intersect in terms of their relative position to the thing described by that Relative =cut sub intersection { # overriding the RangeI implementation so we can transfer map and handle # Relative my ($self, $given, $so, $rel) = @_; $self->throw("missing arg: you need to pass in another argument") unless $given; my @positions; if ($self eq "Bio::Map::PositionI") { $self = "Bio::Map::Position"; $self->warn("calling static methods of an interface is deprecated; use $self instead"); } if (ref $self) { push(@positions, $self); } ref($given) eq 'ARRAY' ? push(@positions, @{$given}) : push(@positions, $given); $self->throw("Need at least 2 Positions") unless @positions >= 2; my ($intersect, $i_start, $i_end, $c_start, $c_end, %known_maps); while (@positions > 0) { unless ($intersect) { $intersect = shift(@positions); ($i_start, $i_end) = $self->_pre_rangei($intersect, $rel); my $map = $intersect->map; $known_maps{$map->unique_id} = $map; } my $compare = shift(@positions); ($c_start, $c_end) = $self->_pre_rangei($compare, $rel); return unless $compare->_testStrand($intersect, $so); if ($compare->isa('Bio::Map::PositionI')) { my $this_map = $compare->map; if ($this_map) { $known_maps{$this_map->unique_id} = $this_map; } } else { $self->throw("Only Bio::Map::PositionI objects are supported, not [$compare]"); } my @starts = sort {$a <=> $b} ($i_start, $c_start); my @ends = sort {$a <=> $b} ($i_end, $c_end); my $start = pop @starts; # larger of the 2 starts my $end = shift @ends; # smaller of the 2 ends my $intersect_strand; # strand for the intersection if (defined($intersect->strand) && defined($compare->strand) && $intersect->strand == $compare->strand) { $intersect_strand = $compare->strand; } else { $intersect_strand = 0; } if ($start > $end) { return; } else { $intersect = $self->new(-start => $start, -end => $end, -strand => $intersect_strand); } } $intersect || return; my ($start, $end, $strand) = ($intersect->start, $intersect->end, $intersect->strand); my @intersects; foreach my $known_map (values %known_maps) { my $new_intersect = $intersect->new(-start => $start, -end => $end, -strand => $strand, -map => $known_map); $new_intersect->relative($rel) if $rel; push(@intersects, $new_intersect); } unless (@intersects) { $intersect->relative($rel) if $rel; @intersects = ($intersect); } my $result = Bio::Map::Mappable->new(); $result->add_position(@intersects); # sneaky, add_position can take a list of positions return $result; } =head2 union Title : union Usage : ($start, $stop, $strand) = $p1->union($p2); ($start, $stop, $strand) = Bio::Map::Position->union(@positions); my $mappable = $p1->union($p2); my $mappable = Bio::Map::Position->union(@positions); Function: finds the minimal position/range that contains all of the positions Returns : Bio::Map::Mappable object who's positions are the cross-map-calculated union of the input positions on all the maps that the input positions belong to, OR, in list context, a three element array (start, end, strand) Args : a Bio::Map::PositionI to compare this one to, or a list of such OR a single Bio::Map::PositionI or array ref of such AND a Bio::Map::RelativeI to ask for the Position's union in terms of their relative position to the thing described by that Relative =cut sub union { # overriding the RangeI implementation so we can transfer map and handle # Relative my ($self, @args) = @_; $self->throw("Not enough arguments") unless @args >= 1; my @positions; my $rel; if ($self eq "Bio::Map::PositionI") { $self = "Bio::Map::Position"; $self->warn("calling static methods of an interface is deprecated; use $self instead"); } if (ref $self) { push(@positions, $self); } if (ref $args[0] eq 'ARRAY') { push(@positions, @{shift(@args)}); } else { push(@positions, shift(@args)); } if ($args[0] && $args[0]->isa('Bio::Map::RelativeI')) { $rel = shift(@args); } foreach my $arg (@args) { # avoid pushing undefined values into @positions push(@positions, $arg) if $arg; } $self->throw("Need at least 2 Positions") unless @positions >= 2; my (@starts, @ends, %known_maps, $union_strand); foreach my $compare (@positions) { # RangeI union allows start or end to be undefined; however _pre_rangei # will throw my ($start, $end) = $self->_pre_rangei($compare, $rel); if ($compare->isa('Bio::Map::PositionI')) { my $this_map = $compare->map; if ($this_map) { $known_maps{$this_map->unique_id} = $this_map; } } else { $self->throw("Only Bio::Map::PositionI objects are supported, not [$compare]"); } if (! defined $union_strand) { $union_strand = $compare->strand; } else { if (! defined $compare->strand or $union_strand ne $compare->strand) { $union_strand = 0; } } push(@starts, $start); push(@ends, $end); } @starts = sort { $a <=> $b } @starts; @ends = sort { $a <=> $b } @ends; my $start = shift @starts; my $end = pop @ends; my @unions; foreach my $known_map (values %known_maps) { my $new_union = $self->new(-start => $start, -end => $end, -strand => $union_strand, -map => $known_map); $new_union->relative($rel) if $rel; push(@unions, $new_union); } unless (@unions) { @unions = ($self->new(-start => $start, -end => $end, -strand => $union_strand)); $unions[0]->relative($rel) if $rel; } my $result = Bio::Map::Mappable->new(); $result->add_position(@unions); # sneaky, add_position can take a list of positions return $result; } =head2 overlap_extent Title : overlap_extent Usage : ($a_unique,$common,$b_unique) = $a->overlap_extent($b) Function: Provides actual amount of overlap between two different positions Example : Returns : array of values containing the length unique to the calling position, the length common to both, and the length unique to the argument position Args : a position =cut #*** should this be overridden from RangeI? =head2 disconnected_ranges Title : disconnected_ranges Usage : my @disc_ranges = Bio::Map::Position->disconnected_ranges(@ranges); Function: Creates the minimal set of positions such that each input position is fully contained by at least one output position, and none of the output positions overlap. Returns : Bio::Map::Mappable with the calculated disconnected ranges Args : a Bio::Map::PositionI to compare this one to, or a list of such, OR a single Bio::Map::PositionI or array ref of such AND a Bio::Map::RelativeI to consider all Position's co-ordinates in terms of their relative position to the thing described by that Relative, AND, optionally, an int for the minimum percentage of overlap that must be present before considering two ranges to be overlapping (default 0) =cut sub disconnected_ranges { # overriding the RangeI implementation so we can transfer map and handle # Relative my ($self, @args) = @_; $self->throw("Not enough arguments") unless @args >= 1; my @positions; my $rel; my $overlap = 0; if ($self eq "Bio::Map::PositionI") { $self = "Bio::Map::Position"; $self->warn("calling static methods of an interface is deprecated; use $self instead"); } if (ref $self) { push(@positions, $self); } if (ref $args[0] eq 'ARRAY') { push(@positions, @{shift(@args)}); } else { push(@positions, shift(@args)); } if ($args[0] && $args[0]->isa('Bio::Map::RelativeI')) { $rel = shift(@args); $overlap = shift(@args); } foreach my $arg (@args) { push(@positions, $arg) if $arg; } $self->throw("Need at least 2 Positions") unless @positions >= 2; my %known_maps; foreach my $pos (@positions) { $pos->isa('Bio::Map::PositionI') || $self->throw("Must supply only Bio::Map::PositionI objects, not [$pos]"); my $map = $pos->map || next; $known_maps{$map->unique_id} = $map; } my %prior_positions; foreach my $map (values %known_maps) { foreach my $pos ($map->get_positions) { $prior_positions{$pos} = 1; } } my @outranges = (); foreach my $inrange (@positions) { my @outranges_new = (); my %overlapping_ranges = (); for (my $i=0; $i<@outranges; $i++) { my $outrange = $outranges[$i]; if ($inrange->overlaps($outrange, undef, $rel, $overlap)) { my $union_able = $inrange->union($outrange, $rel); # using $inrange->union($outrange, $rel); gives >6x speedup, # but different answer, not necessarily incorrect... foreach my $pos ($union_able->get_positions) { $overlapping_ranges{$pos->toString} = $pos; # we flatten down to a result on a single map # to avoid creating 10s of thousands of positions during this process; # we then apply the final answer to all maps at the very end last; } } else { push(@outranges_new, $outrange); } } @outranges = @outranges_new; my @overlappers = values %overlapping_ranges; if (@overlappers) { if (@overlappers > 1) { my $merged_range_able = shift(@overlappers)->union(\@overlappers, $rel); push(@outranges, $merged_range_able->get_positions); } else { push(@outranges, @overlappers); } } else { push(@outranges, $self->new(-start => $inrange->start($rel), -end => $inrange->end($rel), -strand => $inrange->strand, -map => $inrange->map, -relative => $rel)); } } # purge positions that were created whilst calculating the answer, but # aren't the final answer and weren't there previously my %answers = map { $_ => 1 } @outranges; foreach my $map (values %known_maps) { foreach my $pos ($map->get_positions) { if (! exists $prior_positions{$pos} && ! exists $answers{$pos}) { $map->purge_positions($pos); } } } my %post_positions; foreach my $map (values %known_maps) { foreach my $pos ($map->get_positions) { $post_positions{$pos} = 1; } } @outranges || return; # make an outrange on all known maps my @final_positions; foreach my $map (values %known_maps) { foreach my $pos (@outranges) { if ($pos->map eq $map) { push(@final_positions, $pos); } else { push(@final_positions, $pos->new(-start => $pos->start, -end => $pos->end, -relative => $pos->relative, -map => $map)); } } } # assign the positions to a result mappable my $result = Bio::Map::Mappable->new(); $result->add_position(@final_positions); # sneaky, add_position can take a list of positions return $result; } # get start & end suitable for rangeI methods, taking relative into account sub _pre_rangei { my ($self, $other, $rel) = @_; $self->throw("Must supply an object") unless $other; if ($rel) { $self->throw("Must supply an object for the Relative argument") unless ref($rel); $self->throw("This is [$rel], not a Bio::Map::RelativeI") unless $rel->isa('Bio::Map::RelativeI'); } my ($other_start, $other_end); if (ref($other)) { if (ref($other) eq 'ARRAY') { $self->throw("_pre_rangei got an array"); } $self->throw("This is [$other], not a Bio::RangeI object") unless defined $other && $other->isa('Bio::RangeI'); if ($other->isa('Bio::Map::PositionI')) { # to get the desired start/end we need the position to be on a map; # if it isn't on one temporarily place it on self's map # - this lets us have 'generic' positions that aren't on any map # but have a relative defined and can thus be usefully compared to # positions that /are/ on maps my $other_map = $other->map; unless ($other_map) { my $self_map = $self->map || $self->throw("Trying to compare two positions but neither had been placed on a map"); $other->map($self_map); } # want start and end positions relative to the supplied rel or map start $rel ||= $other->absolute_relative; $other_start = $other->start($rel); $other_end = $other->end($rel); unless ($other_map) { $self->map->purge_positions($other); } } else { $other_start = $other->start; $other_end = $other->end; } } else { $self->throw("not a number") unless looks_like_number($other); $other_start = $other_end = $other; } $other->throw("start is undefined") unless defined $other_start; $other->throw("end is undefined") unless defined $other_end; return ($other_start, $other_end); } 1;