# $Id: scoring.pm 16123 2009-09-17 12:57:27Z cjfields $ # # BioPerl module for Bio::Matrix::IO::scoring # # Please direct questions and support issues to # # Cared for by Jason Stajich # # Copyright Jason Stajich # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code =head1 NAME Bio::Matrix::IO::scoring - A parser for PAM/BLOSUM matricies =head1 SYNOPSIS use Bio::Matrix::IO; my $parser = Bio::Matrix::IO->new(-format => 'scoring', -file => 'BLOSUM50'); my $matrix = $parser->next_matrix; =head1 DESCRIPTION Describe the object here =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHOR - Jason Stajich Email jason-at-bioperl-dot-org =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::Matrix::IO::scoring; use strict; use Bio::Matrix::Scoring; use base qw(Bio::Matrix::IO); =head2 new Title : new Usage : my $obj = Bio::Matrix::IO::scoring->new(); Function: Builds a new Bio::Matrix::IO::scoring object Returns : an instance of Bio::Matrix::IO::scoring Args : =cut =head2 next_matrix Title : next_matrix Usage : my $matrux = $parser->next_matrix Function: parses a scoring matrix (BLOSUM,PAM styles) Returns : L Args : none =cut sub next_matrix{ my ($self) = @_; local ($_); my (@matrix,@cols,@rows,%extras,$inmatrix); while( defined ( $_ = $self->_readline ) ) { next if ( /^\s*$/); if( /^\#/ ) { if( $inmatrix ) { $self->_pushback($_); last; } if( m/Entropy\s+\=\s+(\S+)\,\s+ Expected\s+\=\s+(\S+)/ox ) { $extras{'-entropy'} = $1; $extras{'-expected'} = $2; } elsif ( m/Expected\s+score\s+\=\s+(\S+)\, \s+Entropy\s+\=\s+(\S+)/xo ){ $extras{'-entropy'} = $2; $extras{'-expected'} = $1; } elsif( m/(PAM\s+\d+)\s+substitution.+ scale\s+\=\s+(\S+)\s+\=\s+(\S+)/ox ) { $extras{'-matrix_name'} = $1; $extras{'-scale'} = $2; $extras{'-scale_value'} = $3; } elsif( /Blocks Database\s+\=\s+(\S+)/o ) { $extras{'-database'} = $1; } elsif( m/(\S+)\s+Bit\s+Units/ox ) { $extras{'-scale'} = $1; } elsif( m/Lowest score\s+\=\s+(\S+)\,\s+ Highest score\s+\=\s+(\S+)/ox ) { $extras{'-lowest_score'} = $1; $extras{'-highest_score'} = $2; } elsif( m/(Lambda)\s+\=\s+(\S+)\s+bits\, \s+(H)\s+\=\s+(\S+)/ox ) { # This is a DNA matrix $extras{$1} = $2; $extras{$3} = $4; } } elsif( s/^\s+(\S+)/$1/ ) { @cols = split; if( $cols[0] ne 'A' ) { $self->warn("Unrecognized first line of matrix, we might not have parsed it correctly"); } $inmatrix = 1; } elsif( $inmatrix ) { if( ! /^(\S+)/ ) { $inmatrix = 0; next } my ($rowname,@row) = split; push @rows, $rowname; push @matrix, [@row]; } else { print; } } my $matrix = Bio::Matrix::Scoring->new(-values => \@matrix, -rownames => \@rows, -colnames => \@cols, %extras); } =head2 write_matrix Title : write_matrix Usage : $matio->write_matrix($matrix) Function: Write out a matrix in the BLOSUM/PAM format Returns : none Args : L =cut sub write_matrix{ my ($self,@args) = @_; $self->warn("cannot actually use this function yet - it isn't finished"); return; } 1;