# $Id: InstanceSiteI.pm 16123 2009-09-17 12:57:27Z cjfields $ =head1 NAME Bio::Matrix::PSM::InstanceSiteI - InstanceSite interface, holds an instance of a PSM =head1 SYNOPSIS use Bio::Matrix::PSM::InstanceSite; #Y ou can get an InstanceSite object either from a file: my ($instances,$matrix)=$SomePSMFile->parse_next; #or from memory my %params=(seq => 'TATAAT', id => "TATAbox1", accession => 'ENSG00000122304', mid => 'TB1', desc => 'TATA box, experimentally verified in PRM1 gene', relpos => -35); =head1 DESCRIPTION Abstract interface to PSM site occurrence (PSM sequence match). InstanceSite objects may be used to describe a PSM (See Bio::Matrix::PSM::SiteMatrix) sequence matches. The usual characteristic of such a match is sequence coordinates, score, sequence and sequence (gene) identifier- accession number or other id. This object inherits from Bio::LocatableSeq (which defines the real sequence) and might hold a SiteMatrix object, used to detect the CRE (cis-regulatory element), or created from this CRE. While the documentation states that the motif id and gene id (accession) combination should be unique, this is not entirely true- there might be more than one occurrence of the same cis-regulatory element in the upstream region of the same gene. Therefore relpos would be the third element to create a really unique combination. =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHOR - Stefan Kirov Email skirov@utk.edu =head1 SEE ALSO L, L, L =head1 APPENDIX =cut # Let the code begin... package Bio::Matrix::PSM::InstanceSiteI; use strict; use base qw(Bio::Root::RootI); =head2 mid Title : mid Usage : my $mid=$instance->mid; Function: Get/Set the motif id Throws : Returns : scalar Args : scalar =cut sub mid { my $self = shift; $self->throw_not_implemented(); } =head2 score Title : score Usage : my $score=$instance->score; Function: Get/Set the score (mismatches) between the instance and the attached (or initial) PSM Throws : Returns : real number Args : real number =cut sub score { my $self = shift; $self->throw_not_implemented(); } =head2 start Title : start Usage : my $start=$instance->start; Function: Get/Set the position of the instance on the sequence used Throws : Returns : integer Args : integer =cut sub start { my $self = shift; $self->throw_not_implemented(); } =head2 relpos Title : relpos Usage : my $seqpos=$instance->relpos; Function: Get/Set the relative position of the instance with respect to the transcription start site (if known). Can and usually is negative. Throws : Returns : integer Args : integer =cut sub relpos { my $self = shift; $self->throw_not_implemented(); } =head2 minstance Title : minstance Usage : my $minstance=$misntance->score; Function: Get/Set the unique identifier- sequence id/motif id, for example PRM1_TATAbox. Not necessarily human readable. Throws : Returns : string Args : string =cut sub minstance { my $self = shift; $self->throw_not_implemented(); } 1;