# $Id: csv.pm 16123 2009-09-17 12:57:27Z cjfields $ # # BioPerl module for Bio::PopGen::IO::csv # # Please direct questions and support issues to # # Cared for by Jason Stajich # # Copyright Jason Stajich # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code =head1 NAME Bio::PopGen::IO::csv -Extract individual allele data from a CSV parser =head1 SYNOPSIS #Do not use directly, use through the Bio::PopGen::IO driver use Bio::PopGen::IO; my $io = Bio::PopGen::IO->new(-format => 'csv', -file => 'data.csv'); # Some IO might support reading in a population at a time my @population; while( my $ind = $io->next_individual ) { push @population, $ind; } =head1 DESCRIPTION This object will parse comma delimited format (CSV) or whatever delimiter you specify. It currently doesn't handle the more complex quote escaped CSV format. There are 3 initialization parameters, the delimiter (-field_delimiter) [default ','], (-allele_delimiter) [default ' ']. The third initialization parameter is a boolean -no_header which specifies if there is no header line to read in. All lines starting with '#' will be skipped When no_header is not specific the data is assumed to be of the following form. Having a header line this SAMPLE,MARKERNAME1,MARKERNAME2,... and each data line having the form (diploid data) SAMP1,101 102,100 90,a b or for haploid data SAMP1,101,100,a =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHOR - Jason Stajich Email jason-at-bioperl.org =head1 CONTRIBUTORS Matthew Hahn, matthew.hahn-at-duke.edu =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::PopGen::IO::csv; use vars qw($FieldDelim $AlleleDelim $NoHeader); use strict; ($FieldDelim,$AlleleDelim,$NoHeader) =( ',', '\s+',0); # Object preamble - inherits from Bio::Root::Root use Bio::PopGen::Individual; use Bio::PopGen::Population; use Bio::PopGen::Genotype; use base qw(Bio::PopGen::IO); =head2 new Title : new Usage : my $obj = Bio::PopGen::IO::csv->new(); Function: Builds a new Bio::PopGen::IO::csv object Returns : an instance of Bio::PopGen::IO::csv Args : [optional, these are the current defaults] -field_delimiter => ',' -allele_delimiter=> '\s+' -no_header => 0, =cut sub _initialize { my($self, @args) = @_; my ($fieldsep,$all_sep, $noheader) = $self->_rearrange([qw(FIELD_DELIMITER ALLELE_DELIMITER NO_HEADER)],@args); $self->flag('no_header', defined $noheader ? $noheader : $NoHeader); $self->flag('field_delimiter',defined $fieldsep ? $fieldsep : $FieldDelim); $self->flag('allele_delimiter',defined $all_sep ? $all_sep : $AlleleDelim); $self->{'_header'} = undef; return 1; } =head2 flag Title : flag Usage : $obj->flag($flagname,$newval) Function: Get/Set the flag value Returns : value of a flag (a boolean) Args : A flag name, currently we expect 'no_header', 'field_delimiter', or 'allele_delimiter' on set, new value (a boolean or undef, optional) =cut sub flag{ my $self = shift; my $fieldname = shift; return unless defined $fieldname; return $self->{'_flag'}->{$fieldname} = shift if @_; return $self->{'_flag'}->{$fieldname}; } =head2 next_individual Title : next_individual Usage : my $ind = $popgenio->next_individual; Function: Retrieve the next individual from a dataset Returns : L object Args : none =cut sub next_individual{ my ($self) = @_; while( defined( $_ = $self->_readline) ) { next if( /^\s*\#/ || /^\s+$/ || ! length($_) ); last; } return if ! defined $_; if( $self->flag('no_header') || defined $self->{'_header'} ) { #########new (allows field delim to be the same as the allele delim my ($samp,@marker_results); if($self->flag('field_delimiter') ne $self->flag('allele_delimiter')){ ($samp,@marker_results) = split($self->flag('field_delimiter'),$_); } else{ my $fielddelim = $self->flag('field_delimiter'); my $alleledelim = $self->flag('allele_delimiter'); ($samp) = /(^.+?)$fielddelim/; s/^.+?$fielddelim//; (@marker_results) = /([\d|\w]+$alleledelim[\d|\w]+)/g; } #########end new my $i = 1; foreach my $m ( @marker_results ) { $m =~ s/^\s+//; $m =~ s/\s+$//; my $markername; if( defined $self->{'_header'} ) { $markername = $self->{'_header'}->[$i]; } else { $markername = "Marker$i"; } $self->debug( "markername is $markername alleles are $m\n"); my @alleles = split($self->flag('allele_delimiter'), $m); $m = Bio::PopGen::Genotype->new(-alleles => \@alleles, -marker_name => $markername, -individual_id=> $samp); $i++; } return Bio::PopGen::Individual->new(-unique_id => $samp, -genotypes => \@marker_results); } else { chomp; $self->{'_header'} = [split($self->flag('field_delimiter'),$_)]; return $self->next_individual; # rerun loop again } return; } =head2 next_population Title : next_population Usage : my $ind = $popgenio->next_population; Function: Retrieve the next population from a dataset Returns : L object Args : none Note : Many implementation will not implement this =cut # Plan is to just return the whole dataset as a single population by # default I think - people would then have each population in a separate # file. sub next_population{ my ($self) = @_; my @inds; while( my $ind = $self->next_individual ) { push @inds, $ind; } Bio::PopGen::Population->new(-individuals => \@inds); } =head2 write_individual Title : write_individual Usage : $popgenio->write_individual($ind); Function: Write an individual out in the file format Returns : none Args : L object(s) =cut sub write_individual{ my ($self,@inds) = @_; my $fielddelim = $self->flag('field_delimiter'); my $alleledelim= $self->flag('allele_delimiter'); foreach my $ind ( @inds ) { if (! ref($ind) || ! $ind->isa('Bio::PopGen::IndividualI') ) { $self->warn("Cannot write an object that is not a Bio::PopGen::IndividualI object ($ind)"); next; } # we'll go ahead and sort these until # we have a better way to insure a consistent order my @marker_names = sort $ind->get_marker_names; if( ! $self->flag('no_header') && ! $self->flag('header_written') ) { $self->_print(join($fielddelim, ('SAMPLE', @marker_names)), "\n"); $self->flag('header_written',1); } $self->_print( join($fielddelim, $ind->unique_id, # we're chaining map here, pay attention and read # starting with the last map # we'll turn genotypes into allele pairs # which will be separated by the allele delimiter map { join($alleledelim,$_->get_Alleles) } # marker names will be sorted so we don't # have to worry about this between individuals # unless the individual set you pass in has # a mixed set of markers... # this will turn marker names into Genotypes map {$ind->get_Genotypes(-marker => $_)} @marker_names), "\n") } } =head2 write_population Title : write_population Usage : $popgenio->write_population($pop); Function: Write a population out in the file format Returns : none Args : L object(s) Note : Many implementation will not implement this =cut sub write_population{ my ($self,@pops) = @_; my $fielddelim = $self->flag('field_delimiter'); # my $alleledelim= $self->flag('allele_delimiter'); my $alleledelim = ' '; foreach my $pop ( @pops ) { if (! ref($pop) || ! $pop->isa('Bio::PopGen::PopulationI') ) { $self->warn("Cannot write an object that is not a Bio::PopGen::PopulationI object"); next; } # we'll go ahead and sort these until # we have a better way to insure a consistent order my @marker_names = sort $pop->get_marker_names; if( ! $self->flag('no_header') && ! $self->flag('header_written') ) { $self->_print( join($fielddelim, ('SAMPLE', @marker_names)), "\n"); $self->flag('header_written',1); } foreach my $ind ( $pop->get_Individuals ) { $self->_print( join($fielddelim, $ind->unique_id, # we're chaining map here, pay attention # and read starting with the last map # we'll turn genotypes into allele pairs # which will be separated by the allele # delimiter map { join($alleledelim,$_->get_Alleles) } # marker names will be sorted so we don't # have to worry about this between individuals # unless the individual set you pass in has # a mixed set of markers... # this will turn marker names into Genotypes map {$ind->get_Genotypes(-marker => $_)} @marker_names), "\n"); } } } 1;