# $Id: IO.pm 16123 2009-09-17 12:57:27Z cjfields $ # # BioPerl module for Bio::Restriction::IO # # Please direct questions and support issues to # # Cared for by Rob Edwards # # Copyright Rob Edwards # # You may distribute this module under the same terms as perl itself # # POD documentation - main docs before the code =head1 NAME Bio::Restriction::IO - Handler for sequence variation IO Formats =head1 SYNOPSIS use Bio::Restriction::IO; $in = Bio::Restriction::IO->new(-file => "inputfilename" , -format => 'withrefm'); my $res = $in->read; # a Bio::Restriction::EnzymeCollection =head1 DESCRIPTION L is a handler module for the formats in the Restriction IO set, e.g. C. It is the officially sanctioned way of getting at the format objects, which most people should use. The structure, conventions and most of the code is inherited from L. The main difference is that instead of using methods C, you drop C<_seq> from the method name. Also, instead of dealing only with individual L objects, C will slurp in all enzymes into a L object. For more details, see documentation in L. =head1 TO DO At the moment, these can be use mainly to get a custom set if enzymes in C or C formats into L or L objects. Using C format is highly experimental and is not recommmended at this time. This class inherits from L for convenience sake, though this should inherit from L. Get rid of L inheritance by copying relevant methods in. C methods are currently not implemented for any format except C. Using C even with C format is not recommended as it does not support multicut/multisite enzyme output. Should additional formats be supported (such as XML)? =head1 SEE ALSO L, L, L =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing lists Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHOR Rob Edwards, redwards@utmem.edu =head1 CONTRIBUTORS Heikki Lehvaslaiho, heikki-at-bioperl-dot-org =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::Restriction::IO; use strict; use vars qw(%FORMAT); use base qw(Bio::SeqIO); %FORMAT = ( 'itype2' => 'itype2', '8' => 'itype2', 'withrefm' => 'withrefm', '31' => 'withrefm', 'base' => 'base', '0' => 'base', 'bairoch' => 'bairoch', '19' => 'bairoch', 'macvector' => 'bairoch', 'vectorNTI' => 'bairoch', 'neo' => 'prototype', 'prototype' => 'prototype' ); =head2 new Title : new Usage : $stream = Bio::Restriction::IO->new(-file => $filename, -format => 'Format') Function: Returns a new seqstream Returns : A Bio::Restriction::IO::Handler initialised with the appropriate format Args : -file => $filename -format => format -fh => filehandle to attach to =cut sub new { my ($class, %param) = @_; my ($format); @param{ map { lc $_ } keys %param } = values %param; # lowercase keys $format = $FORMAT{$param{'-format'}} if defined $param{'-format'}; $format ||= $class->_guess_format( $param{-file} || $ARGV[0] ) || 'base'; $format = "\L$format"; # normalize capitalization to lower case return unless $class->_load_format_module($format); return "Bio::Restriction::IO::$format"->new(%param); } sub _load_format_module { my ($class, $format) = @_; my $module = "Bio::Restriction::IO::" . $format; my $ok; eval { $ok = $class->_load_module($module); }; if ( $@ ) { print STDERR <read Function: reads all the restrction enzymes from the stream Returns : a Bio::Restriction::EnzymeCollection object Args : =cut sub read { my ($self, $seq) = @_; $self->throw_not_implemented(); } sub next { my ($self, $seq) = @_; $self->throw_not_implemented(); } sub next_seq { my ($self, $seq) = @_; $self->throw_not_implemented(); } =head2 write Title : write Usage : $stream->write($seq) Function: writes the $seq object into the stream Returns : 1 for success and 0 for error Args : Bio::Restriction::EnzymeCollection object =cut sub write { my ($self, $seq) = @_; $self->throw("Sorry, you cannot write to a generic ". "Bio::Restricion::IO object."); } sub write_seq { my ($self, $seq) = @_; $self->warn("These are not sequence objects. ". "Use method 'write' instead of 'write_seq'."); $self->write($seq); } =head2 _guess_format Title : _guess_format Usage : $obj->_guess_format($filename) Function: Example : Returns : guessed format of filename (lower case) Args : =cut sub _guess_format { my $class = shift; return unless $_ = shift; return 'flat' if /\.dat$/i; return 'xml' if /\.xml$/i; } 1;