# $Id: withrefm.pm 16123 2009-09-17 12:57:27Z cjfields $ # BioPerl module for Bio::Restriction::IO::withrefm # # Please direct questions and support issues to # # Cared for by Rob Edwards # # Copyright Rob Edwards # # You may distribute this module under the same terms as perl itself # # POD documentation - main docs before the code =head1 NAME Bio::Restriction::IO::withrefm - withrefm enzyme set =head1 SYNOPSIS Do not use this module directly. Use it via the Bio::Restriction::IO class. =head1 DESCRIPTION This is the most complete format of the REBASE files, and basically includes all the data on each of the restriction enzymes. =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing lists Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHOR Rob Edwards, redwards@utmem.edu =head1 CONTRIBUTORS Heikki Lehvaslaiho, heikki-at-bioperl-dot-org Mark A. Jensen, maj-at-fortinbras-dot-us =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::Restriction::IO::withrefm; use vars qw(%WITH_REFM_FIELD); use strict; #use Bio::Restriction::IO; use Bio::Restriction::Enzyme; use Bio::Restriction::EnzymeCollection; use Data::Dumper; use base qw(Bio::Restriction::IO::base); =head2 read Title : read Usage : $renzs = $stream->read Function: reads all the restrction enzymes from the stream Returns : a Bio::Restriction::Restriction object Args : none =cut sub read { my $self = shift; my $renzs = Bio::Restriction::EnzymeCollection->new(-empty => 1); local $/ = '<1>'; while (defined(my $entry=$self->_readline()) ) { # not an entry. next unless $entry =~ /<2>/; #$self->debug("|$entry|\n"); # # Minimal information # my ($name) = $entry =~ /^(\S+)/; my ($site) = $entry =~ /\<3\>([^\n]+)/; if ( ! defined $site || $site eq '' or $site eq '?') { $self->warn("$name: no site. Skipping") if $self->verbose > 1; next; } # there are a couple of sequences that have multiple # recognition sites eg M.PhiBssHII: ACGCGT,CCGCGG,RGCGCY,RCCGGY,GCGCGC # TaqII : GACCGA(11/9),CACCCA(11/9) my @sequences; if ($site =~ /\,/) { @sequences = split (/\,/, $site); $site=shift @sequences; } # this regexp now parses all possible components # $1 : (s/t) or undef # $2 : [site] # $3 : (m/n) or undef /maj no warnings; # avoid faulty 'uninitialized value' warnings # occurring against the variables set by # regexp matching (unless anyone has other ideas...) my ($precut, $recog, $postcut) = ( $site =~ m/^(?:\((\w+\/\w+)\))?([\w^]+)(?:\((\w+\/\w+)\))?/ ); # # prototype / isoschizomers # my ($isoschizomers) = $entry =~ /<2>([^\n]+)/; my @isos = split(/\,/,$isoschizomers); my $is_prototype = (@isos ? 1 : 0); # # microbe # my ($microbe) = $entry =~ /<5>([^\n]+)/; # # source # my ($source) = $entry =~ /<6>([^\n]+)/; # # vendors # my ($vendors) = $entry =~ /<7>([^\n]+)/; my @vendors = split(/ */, $vendors); # # references # my ($refs) = $entry =~ /<8>(.+)/s; my @refs = map {split /\n+/} $refs; use warnings; # when enz is constructed, site() will contain original characters, # but recog() will contain a regexp if required.../maj my $re = Bio::Restriction::Enzyme->new( -name => $name, -site => $recog, -recog => $recog, -precut => $precut, -postcut => $postcut, -is_prototype => $is_prototype, -isoschizomers => [@isos], -source => $source, -vendors => [@vendors], -references => [@refs], -xln_sub => \&_xln_sub ); # # methylation: easier to set here during parsing/maj # my ($meth) = $entry =~ /<4>([^\n]+)/; my @meths; if ($meth) { # this can be either X(Y) or X(Y),X2(Y2) # where X is the base and y is the type of methylation if ( $meth =~ /(\S+)\((\d+)\),(\S+)\((\d+)\)/ ) { # two msites per site #my ($p1, $m1, $p2, $m2) = ($1, $2, $3, $4); $re->methylation_sites($self->_meth($re,$1, $2), $self->_meth($re,$3,$4)); } elsif ($meth =~ /(\S+)\((\d+)\)/ ) { # one msite per site or more sites $re->methylation_sites( $self->_meth($re,$1,$2) ); @meths = split (/\, /, $meth); $meth=shift @meths; } else { $self->warn("Unknown methylation format [$meth]") if $self->verbose >0; } } # the _make_multicuts function now takes place in the # Enzyme constructor / maj # # create special types of Enzymes # (because of object cloning in _make_multisites, this happens # after everything else is set /maj) # (with the removal of the collection from the arglist, this # call (or its code) could now be placed in the constructor, # which is safer (since this has to happen last), # but it requires the methylation info, which # is more natural to get out here in the parsing./maj $self->_make_multisites($re, \@sequences, \@meths, \&_xln_sub) if @sequences; $renzs->enzymes($re); } return $renzs; } =head2 _xln_sub Title : _xln_sub Function: Translates withrefm coords to Bio::Restriction coords Args : Bio::Restriction::Enzyme object, scalar integer (cut posn) Note : Used internally; pass as a coderef to the B:R::Enzyme constructor =cut sub _xln_sub { my ($z,$c) = @_; return ($c < 0 ? $c : length($z->string)+$c); } =head2 write Title : write Usage : $stream->write($renzs) Function: writes restriction enzymes into the stream Returns : 1 for success and 0 for error Args : a Bio::Restriction::Enzyme or a Bio::Restriction::EnzymeCollection object =cut sub write { my ($self,@h) = @_; $self->throw_not_implemented; } 1;