# $Id: WABAHSP.pm 16123 2009-09-17 12:57:27Z cjfields $ # # BioPerl module for Bio::Search::HSP::WABAHSP # # Please direct questions and support issues to # # Cared for by Jason Stajich # # Copyright Jason Stajich # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code =head1 NAME Bio::Search::HSP::WABAHSP - HSP object suitable for describing WABA alignments =head1 SYNOPSIS # use this object as you would a GenericHSP # a few other methods have been added including state =head1 DESCRIPTION This object implements a few of the extra methods such as hmmstate_string which returns the HMM state representation for the WABA alignment. We also must implement a method to calculate homology_string since it is not returned by the algorithm in the machine readable format. =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHOR - Jason Stajich Email jason@bioperl.org =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::Search::HSP::WABAHSP; use strict; use Bio::Root::RootI; use base qw(Bio::Search::HSP::GenericHSP); =head2 new Title : new Usage : my $obj = Bio::Search::HSP::WABAHSP->new(); Function: Builds a new Bio::Search::HSP::WABAHSP object Returns : Bio::Search::HSP::WABAHSP Args : -hmmstate_seq => the string representing the state output from WABA =cut sub new { my($class,@args) = @_; # gotta do some preprocessing before we send the arguments to the superclass my ($len,$qs,$hs) = Bio::Root::RootI->_rearrange([qw(HSP_LENGTH QUERY_SEQ HIT_SEQ)],@args); if( $len != length($qs) ) { Bio::Root::RootI->warn("HSP_LENGTH must equal length of query_seq string, using value from QUERY_SEQ\n"); $len = length($qs); } my( $homol_seq,$gapct,$identical) = ('',0,0); for(my $i=0;$i<$len;$i++) { my $q = substr($qs,$i,1); my $h = substr($hs,$i,1); if( $q eq '-' || $h eq '-' ) { $homol_seq .= ' '; $gapct ++; } elsif( $q eq $h ) { $homol_seq .= '|'; $identical++; } else { $homol_seq .= ' '; } } my $self = $class->SUPER::new('-conserved' => $identical, '-identical' => $identical, '-gaps' => $gapct, '-homology_seq' => $homol_seq, @args); my ($hmmst) = $self->_rearrange([qw(HMMSTATE_SEQ)],@args); defined $hmmst && $self->hmmstate_string($hmmst); $self->add_tag_value('Target' , join(" ","Sequence:".$self->hit->seq_id, $self->hit->start, $self->hit->end)); return $self; } =head2 hmmstate_string Title : hmmstate_string Usage : my $hmmseq = $wabahsp->hmmstate_string(); Function: Get/Set the WABA HMM stateseq Returns : string Args : [optional] string =cut sub hmmstate_string{ my ($self,$val) = @_; if( defined $val ) { $self->{'_hmmstate_string'} = $val; } return $self->{'_hmmstate_string'}; } =head2 homology_string Title : homolgy_string Usage : my $homology_str = $hsp->homology_string(); Function: Homology string must be calculated for a WABA HSP so we can do so here and cache the result so it is only done once Returns : string Args : none =cut sub homology_string{ my ($self) = @_; return ''; } 1;