# $Id: BlastHit.pm 16123 2009-09-17 12:57:27Z cjfields $ # # BioPerl module for Bio::Search::Hit::GenericHit # # Please direct questions and support issues to # # Cared for by Jason Stajich # # Copyright Jason Stajich # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code =head1 NAME Bio::Search::Hit::BlastHit - Blast-specific subclass of Bio::Search::Hit::GenericHit =head1 SYNOPSIS use Bio::Search::Hit::BlastHit; my $hit = Bio::Search::Hit::BlastHit->new(-algorithm => 'blastp'); # See Bio::Search::Hit::GenericHit for information about working with Hits. # TODO: Describe how to configure a SearchIO stream so that it generates # GenericHit objects. =head1 DESCRIPTION This object is a subclass of Bio::Search::Hit::GenericHit and provides some operations that facilitate working with BLAST and PSI-BLAST Hits. For general information about working with Hits, see Bio::Search::Hit::GenericHit. =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHOR - Jason Stajich and Steve Chervitz Email jason@bioperl.org Email sac@bioperl.org =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::Search::Hit::BlastHit; use strict; use Bio::Search::SearchUtils; use base qw(Bio::Search::Hit::GenericHit); =head2 new Title : new Usage : my $obj = Bio::Search::Hit::GenericHit->new(); Function: Builds a new Bio::Search::Hit::GenericHit object Returns : Bio::Search::Hit::GenericHit Args : See Bio::Search::Hit::GenericHit() for other args. Here are the BLAST-specific args that can be used when creating BlastHit objects: -iteration => integer for the PSI-Blast iteration number -found_again => boolean, true if hit appears in a "previously found" section of a PSI-Blast report. =cut sub new { my($class,@args) = @_; my $self = $class->SUPER::new(@args); my ($iter,$found) = $self->_rearrange([qw(ITERATION FOUND_AGAIN )], @args); defined $iter && $self->iteration($iter); defined $found && $self->found_again($found); return $self; } =head2 iteration Usage : $hit->iteration( $iteration_num ); Purpose : Gets the iteration number in which the Hit was found. Example : $iteration_num = $sbjct->iteration(); Returns : Integer greater than or equal to 1 Non-PSI-BLAST reports will report iteration as 1, but this number is only meaningful for PSI-BLAST reports. Argument : iteration_num (optional, used when setting only) Throws : none See Also : L =cut sub iteration{ my ($self,$value) = @_; if( defined $value) { $self->{'_psiblast_iteration'} = $value; } return $self->{'_psiblast_iteration'}; } =head2 found_again Title : found_again Usage : $hit->found_again; $hit->found_again(1); Purpose : Gets a boolean indicator whether or not the hit has been found in a previous iteration. This is only applicable to PSI-BLAST reports. This method indicates if the hit was reported in the "Sequences used in model and found again" section of the PSI-BLAST report or if it was reported in the "Sequences not found previously or not previously below threshold" section of the PSI-BLAST report. Only for hits in iteration > 1. Example : if( $hit->found_again()) { ... }; Returns : Boolean, true (1) if the hit has been found in a previous PSI-BLAST iteration. Returns false (0 or undef) for hits that have not occurred in a previous PSI-BLAST iteration. Argument : Boolean (1 or 0). Only used for setting. Throws : none See Also : L =cut sub found_again { my $self = shift; return $self->{'_found_again'} = shift if @_; return $self->{'_found_again'}; } sub expect { shift->significance(@_) } 1;