# $Id: SimilarityPair.pm 16123 2009-09-17 12:57:27Z cjfields $ # # BioPerl module for Bio::SeqFeature::SimilarityPair # # Please direct questions and support issues to # # Cared for by Hilmar Lapp # # Copyright Hilmar Lapp # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code =head1 NAME Bio::SeqFeature::SimilarityPair - Sequence feature based on the similarity of two sequences. =head1 SYNOPSIS $sim_pair = Bio::SeqFeature::SimilarityPair->from_searchResult($blastHit); $sim = $sim_pair->query(); # a Bio::SeqFeature::Similarity object - the query $sim = $sim_pair->hit(); # dto - the hit. # some properties for the similarity pair $expect = $sim_pair->significance(); $score = $sim_pair->score(); $bitscore = $sim_pair->bits(); # this will not write the description for the sequence (only its name) print $sim_pair->query()->gff_string(), "\n"; =head1 DESCRIPTION Lightweight similarity search result as a pair of Similarity features. This class inherits off Bio::SeqFeature::FeaturePair and therefore implements Bio::SeqFeatureI, whereas the two features of the pair are descendants of Bio::SeqFeature::Generic, with better support for representing similarity search results in a cleaner way. =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHOR - Hilmar Lapp Email hlapp@gmx.net or hilmar.lapp@pharma.novartis.com =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::SeqFeature::SimilarityPair; use strict; use Bio::SeqFeature::Similarity; use Bio::Factory::ObjectFactory; use base qw(Bio::SeqFeature::FeaturePair); =head2 new Title : new Usage : my $similarityPair = Bio::SeqFeature::SimilarityPair->new (-hit => $hit, -query => $query, -source => 'blastp'); Function: Initializes a new SimilarityPair object Returns : Bio::SeqFeature::SimilarityPair Args : -query => The query in a Feature pair -hit => (formerly '-subject') the subject/hit in a Feature pair =cut sub new { my($class,@args) = @_; if(! grep { lc($_) eq "-feature_factory"; } @args) { # if no overriding factory is provided, provide our preferred one my $fact = Bio::Factory::ObjectFactory->new( -type => "Bio::SeqFeature::Similarity", -interface => "Bio::SeqFeatureI"); push(@args, '-feature_factory', $fact); } my $self = $class->SUPER::new(@args); my ($primary, $hit, $query, $fea1, $source,$sbjct) = $self->_rearrange([qw(PRIMARY HIT QUERY FEATURE1 SOURCE SUBJECT )],@args); if( $sbjct ) { # undeprecated by Jason before 1.1 release # $self->deprecated("use of -subject deprecated: SimilarityPair now uses 'hit'"); if(! $hit) { $hit = $sbjct } else { $self->warn("-hit and -subject were specified, using -hit and ignoring -subject"); } } # set the query and subject feature if provided $self->query( $query) if $query && ! $fea1; $hit && $self->hit($hit); # the following refer to feature1, which is guaranteed to exist if( defined $primary || ! defined $self->primary_tag) { $primary = 'similarity' unless defined $primary; $self->primary_tag($primary); } $source && $self->source_tag($source); return $self; } # # Everything else is just inherited from SeqFeature::FeaturePair. # =head2 query Title : query Usage : $query_feature = $obj->query(); $obj->query($query_feature); Function: The query object for this similarity pair Returns : Bio::SeqFeature::Similarity Args : [optional] Bio::SeqFeature::Similarity See L, L =cut sub query { return shift->feature1(@_); } =head2 subject Title : subject Usage : $sbjct_feature = $obj->subject(); $obj->subject($sbjct_feature); Function: Get/Set Subject for a SimilarityPair Returns : Bio::SeqFeature::Similarity Args : [optional] Bio::SeqFeature::Similarity Notes : Deprecated. Use the method 'hit' instead =cut sub subject { my $self = shift; # $self->deprecated("Method subject deprecated: use hit() instead"); $self->hit(@_); } =head2 hit Title : hit Usage : $sbjct_feature = $obj->hit(); $obj->hit($sbjct_feature); Function: Get/Set Hit for a SimilarityPair Returns : Bio::SeqFeature::Similarity Args : [optional] Bio::SeqFeature::Similarity =cut sub hit { return shift->feature2(@_); } =head2 source_tag Title : source_tag Usage : $source = $obj->source_tag(); # i.e., program $obj->source_tag($evalue); Function: Gets the source tag (program name typically) for a feature Returns : string Args : [optional] string =cut sub source_tag { my ($self, @args) = @_; if(@args) { $self->hit()->source_tag(@args); } return $self->query()->source_tag(@args); } =head2 significance Title : significance Usage : $evalue = $obj->significance(); $obj->significance($evalue); Function: Returns : Args : =cut sub significance { my ($self, @args) = @_; if(@args) { $self->hit()->significance(@args); } return $self->query()->significance(@args); } =head2 score Title : score Usage : $score = $obj->score(); $obj->score($value); Function: Returns : Args : =cut sub score { my ($self, @args) = @_; if(@args) { $self->hit()->score(@args); } # Note: You might think it's only getting set on the hit object. # Actually, it's getting set on both hit and query. return $self->query()->score(@args); } =head2 bits Title : bits Usage : $bits = $obj->bits(); $obj->bits($value); Function: Returns : Args : =cut sub bits { my ($self, @args) = @_; if(@args) { $self->hit()->bits(@args); } return $self->query()->bits(@args); } ################################################################# # aliases for backwards compatibility or convenience # ################################################################# *sbjct = \&subject; 1;