# $Id: fasta.pm 16123 2009-09-17 12:57:27Z cjfields $ # BioPerl module for Bio::SeqIO::fasta # # Please direct questions and support issues to # # Cared for by Ewan Birney # and Lincoln Stein # # Copyright Ewan Birney & Lincoln Stein # # You may distribute this module under the same terms as perl itself # _history # October 18, 1999 Largely rewritten by Lincoln Stein # POD documentation - main docs before the code =head1 NAME Bio::SeqIO::fasta - fasta sequence input/output stream =head1 SYNOPSIS Do not use this module directly. Use it via the Bio::SeqIO class. =head1 DESCRIPTION This object can transform Bio::Seq objects to and from fasta flat file databases. =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHORS - Ewan Birney & Lincoln Stein Email: birney@ebi.ac.uk lstein@cshl.org =head1 CONTRIBUTORS Jason Stajich, jason-at-bioperl.org =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::SeqIO::fasta; use vars qw($WIDTH @SEQ_ID_TYPES $DEFAULT_SEQ_ID_TYPE); use strict; use Bio::Seq::SeqFactory; use Bio::Seq::SeqFastaSpeedFactory; use base qw(Bio::SeqIO); @SEQ_ID_TYPES = qw(accession accession.version display primary); $DEFAULT_SEQ_ID_TYPE = 'display'; BEGIN { $WIDTH = 60} sub _initialize { my($self,@args) = @_; $self->SUPER::_initialize(@args); my ($width) = $self->_rearrange([qw(WIDTH)], @args); $width && $self->width($width); unless ( defined $self->sequence_factory ) { $self->sequence_factory(Bio::Seq::SeqFastaSpeedFactory->new()); } } =head2 next_seq Title : next_seq Usage : $seq = $stream->next_seq() Function: returns the next sequence in the stream Returns : Bio::Seq object, or nothing if no more available Args : NONE =cut sub next_seq { my( $self ) = @_; my $seq; my $alphabet; local $/ = "\n>"; return unless my $entry = $self->_readline; chomp($entry); if ($entry =~ m/\A\s*\Z/s) { # very first one return unless $entry = $self->_readline; chomp($entry); } # this just checks the initial input; beyond that, due to setting $/ above, # the > is part of the record separator and is removed $self->throw("The sequence does not appear to be FASTA format ". "(lacks a descriptor line '>')") if $. == 1 && $entry !~ /^>/; $entry =~ s/^>//; my ($top,$sequence) = split(/\n/,$entry,2); defined $sequence && $sequence =~ s/>//g; # my ($top,$sequence) = $entry =~ /^>?(.+?)\n+([^>]*)/s # or $self->throw("Can't parse fasta entry"); my ($id,$fulldesc); if( $top =~ /^\s*(\S+)\s*(.*)/ ) { ($id,$fulldesc) = ($1,$2); } if (defined $id && $id eq '') {$id=$fulldesc;} # FIX incase no space # between > and name \AE defined $sequence && $sequence =~ tr/ \t\n\r//d; # Remove whitespace # for empty sequences we need to know the mol.type $alphabet = $self->alphabet(); if(defined $sequence && length($sequence) == 0) { if(! defined($alphabet)) { # let's default to dna $alphabet = "dna"; } } else { # we don't need it really, so disable # we want to keep this if SeqIO alphabet was set by user # not sure if this could break something #$alphabet = undef; } $seq = $self->sequence_factory->create( -seq => $sequence, -id => $id, # Ewan's note - I don't think this healthy # but obviously to taste. #-primary_id => $id, -desc => $fulldesc, -alphabet => $alphabet, -direct => 1, ); # if there wasn't one before, set the guessed type #unless ( defined $alphabet ) { # don't assume that all our seqs are the same as the first one found #$self->alphabet($seq->alphabet()); #} return $seq; } =head2 write_seq Title : write_seq Usage : $stream->write_seq(@seq) Function: Writes the $seq object into the stream Returns : 1 for success and 0 for error Args : Array of 1 or more Bio::PrimarySeqI objects =cut sub write_seq { my ($self,@seq) = @_; my $width = $self->width; foreach my $seq (@seq) { $self->throw("Did not provide a valid Bio::PrimarySeqI object") unless defined $seq && ref($seq) && $seq->isa('Bio::PrimarySeqI'); my $top; # Allow for different ids my $id_type = $self->preferred_id_type; if( $id_type =~ /^acc/i ) { $top = $seq->accession_number(); if( $id_type =~ /vers/i ) { $top .= "." . $seq->version(); } } elsif($id_type =~ /^displ/i ) { $self->warn("No whitespace allowed in FASTA ID [". $seq->display_id. "]") if defined $seq->display_id && $seq->display_id =~ /\s/; $top = $seq->display_id(); $top = '' unless defined $top; $self->warn("No whitespace allowed in FASTA ID [". $top. "]") if defined $top && $top =~ /\s/; } elsif($id_type =~ /^pri/i ) { $top = $seq->primary_id(); } if ($seq->can('desc') and my $desc = $seq->desc()) { $desc =~ s/\n//g; $top .= " $desc"; } if( $seq->isa('Bio::Seq::LargeSeqI') ) { $self->_print(">$top\n"); # for large seqs, don't call seq(), it defeats the # purpose of the largeseq functionality. instead get # chunks of the seq, $width at a time my $buff_max = 2000; my $buff_size = int($buff_max/$width)*$width; #< buffer is even multiple of widths my $seq_length = $seq->length; my $num_chunks = int($seq_length/$buff_size+1); for( my $c = 0; $c < $num_chunks; $c++ ) { my $buff_end = $buff_size*($c+1); $buff_end = $seq_length if $buff_end > $seq_length; my $buff = $seq->subseq($buff_size*$c+1,$buff_end); if($buff) { $buff =~ s/(.{1,$width})/$1\n/g; $self->_print($buff); } else { $self->_print("\n"); } } } else { my $str = $seq->seq; if(defined $str && length($str) > 0) { $str =~ s/(.{1,$width})/$1\n/g; } else { $str = "\n"; } $self->_print (">",$top,"\n",$str) or return; } } $self->flush if $self->_flush_on_write && defined $self->_fh; return 1; } =head2 width Title : width Usage : $obj->width($newval) Function: Get/Set the line width for FASTA output Returns : value of width Args : newvalue (optional) =cut sub width{ my ($self,$value) = @_; if( defined $value) { $self->{'width'} = $value; } return $self->{'width'} || $WIDTH; } =head2 preferred_id_type Title : preferred_id_type Usage : $obj->preferred_id_type('accession') Function: Get/Set the preferred type of identifier to use in the ">ID" position for FASTA output. Returns : string, one of values defined in @Bio::SeqIO::fasta::SEQ_ID_TYPES. Default = $Bio::SeqIO::fasta::DEFAULT_SEQ_ID_TYPE ('display'). Args : string when setting. This must be one of values defined in @Bio::SeqIO::fasta::SEQ_ID_TYPES. Allowable values: accession, accession.version, display, primary Throws : fatal exception if the supplied id type is not in @SEQ_ID_TYPES. =cut sub preferred_id_type { my ($self,$type) = @_; if( defined $type ) { if( ! grep lc($type) eq $_, @SEQ_ID_TYPES) { $self->throw(-class=>'Bio::Root::BadParameter', -text=>"Invalid ID type \"$type\". Must be one of: @SEQ_ID_TYPES"); } $self->{'_seq_id_type'} = lc($type); # print STDERR "Setting preferred_id_type=$type\n"; } $self->{'_seq_id_type'} || $DEFAULT_SEQ_ID_TYPE; } 1;