# $Id: qual.pm 16123 2009-09-17 12:57:27Z cjfields $ # # Copyright (c) 1997-9 bioperl, Chad Matsalla. All Rights Reserved. # This module is free software; you can redistribute it and/or # modify it under the same terms as Perl itself. # # Copyright Chad Matsalla # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code =head1 NAME Bio::SeqIO::qual - .qual file input/output stream =head1 SYNOPSIS Do not use this module directly. Use it via the Bio::SeqIO class (see L for details). my $in_qual = Bio::SeqIO->new(-file => $qualfile, -format => 'qual', -width => $width, -verbose => $verbose); =head1 DESCRIPTION This object can transform .qual (similar to fasta) objects to and from Bio::Seq::Quality objects. See L for details. Like the fasta module, it can take an argument '-width' to change the number of values per line (defaults to 50). =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHOR Chad Matsalla Chad Matsalla bioinformatics@dieselwurks.com =head1 CONTRIBUTORS Jason Stajich, jason@bioperl.org =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::SeqIO::qual; use strict; use Bio::Seq::SeqFactory; use Dumpvalue; my $dumper = new Dumpvalue(); use base qw(Bio::SeqIO); our $WIDTH = 25; sub _initialize { my($self,@args) = @_; $self->SUPER::_initialize(@args); my ($width) = $self->_rearrange([qw(WIDTH)], @args); $width && $self->width($width); if( ! defined $self->sequence_factory ) { $self->sequence_factory(Bio::Seq::SeqFactory->new (-verbose => $self->verbose(), -type => 'Bio::Seq::PrimaryQual')); } } =head2 next_seq() Title : next_seq() Usage : $scf = $stream->next_seq() Function: returns the next scf sequence in the stream Returns : Bio::Seq::PrimaryQual object Notes : Get the next quality sequence from the stream. =cut sub next_seq { my ($self,@args) = @_; my ($qual,$seq); my $alphabet; local $/ = "\n>"; return unless my $entry = $self->_readline; if ($entry eq '>') { # very first one return unless $entry = $self->_readline; } # original: my ($top,$sequence) = $entry =~ /^(.+?)\n([^>]*)/s my ($top,$sequence) = $entry =~ /^(.+?)\n([^>]*)/s or $self->throw("Can't parse entry [$entry]"); my ($id,$fulldesc) = $top =~ /^\s*(\S+)\s*(.*)/ or $self->throw("Can't parse fasta header"); $id =~ s/^>//; # create the seq object $sequence =~ s/\n+/ /g; return $self->sequence_factory->create (-qual => $sequence, -id => $id, -primary_id => $id, -display_id => $id, -desc => $fulldesc ); } =head2 _next_qual Title : _next_qual Usage : $seq = $stream->_next_qual() (but do not do that. Use $stream->next_seq() instead) Function: returns the next quality in the stream Returns : Bio::Seq::PrimaryQual object Args : NONE Notes : An internal method. Gets the next quality in the stream. =cut sub _next_qual { my $qual = next_primary_qual( $_[0], 1 ); return $qual; } =head2 next_primary_qual() Title : next_primary_qual() Usage : $seq = $stream->next_primary_qual() Function: returns the next sequence in the stream Returns : Bio::PrimaryQual object Args : NONE =cut sub next_primary_qual { # print("CSM next_primary_qual!\n"); my( $self, $as_next_qual ) = @_; my ($qual,$seq); local $/ = "\n>"; return unless my $entry = $self->_readline; if ($entry eq '>') { # very first one return unless $entry = $self->_readline; } my ($top,$sequence) = $entry =~ /^(.+?)\n([^>]*)/s or $self->throw("Can't parse entry [$entry]"); my ($id,$fulldesc) = $top =~ /^\s*(\S+)\s*(.*)/ or $self->throw("Can't parse fasta header"); $id =~ s/^>//; # create the seq object $sequence =~ s/\n+/ /g; if ($as_next_qual) { $qual = Bio::Seq::PrimaryQual->new(-qual => $sequence, -id => $id, -primary_id => $id, -display_id => $id, -desc => $fulldesc ); } return $qual; } =head2 width Title : width Usage : $obj->width($newval) Function: Get/Set the number of values per line for FASTA-like output Returns : value of width Args : newvalue (optional) =cut sub width{ my ($self,$value) = @_; if( defined $value) { $self->{'width'} = $value; } return $self->{'width'} || $WIDTH; } =head2 write_seq Title : write_seq Usage : $obj->write_seq( -source => $source, -header => "some information" -oneline => 0); Function: Write out a list of quality values to a fasta-style file. Returns : Nothing. Args : Requires a reference to a Bio::Seq::Quality object or a PrimaryQual object as the -source. Option 1: information for the header. Option 2: whether the quality score should be on a single line or not Notes : If no -header is provided, $obj->id() will be used where $obj is a reference to either a Quality object or a PrimaryQual object. If $source->id() fails, "unknown" will be the header. If the Quality object has $source->length() of "DIFFERENT" (read the pod, luke), write_seq will use the length of the PrimaryQual object within the Quality object. =cut sub write_seq { my ($self,@args) = @_; my $width = $self->width; my ($source, $head, $oneline) = $self->_rearrange([qw(SOURCE HEADER ONELINE)], @args); if (!$source || ( !$source->isa('Bio::Seq::Quality') && !$source->isa('Bio::Seq::PrimaryQual') )) { $self->throw("You must pass a Bio::Seq::Quality or a Bio::Seq::PrimaryQual". " object to write_seq() as a parameter named \"source\""); } my $header = ($source->can("header") && $source->header) ? $source->header : ($source->can("id") && $source->id) ? $source->id : "unknown"; my @quals = $source->qual(); # ::dumpValue(\@quals); my $desc = $source->desc if $source->can('desc'); $desc ||= ''; $self->_print (">$header $desc\n"); my (@slice,$max,$length); $length = $source->length(); if ( not(defined($oneline)) || $oneline == 0) { # $width quality values per line for (my $count = 1; $count<=$length; $count+= $width) { if ($count+$width > $length) { $max = $length; } else { $max = $count+$width-1; } my @slice = @{$source->subqual($count,$max)}; $self->_print (join(' ',@slice), "\n"); } } else { # quality values on a single line my @slice = @{$source->qual}; $self->_print (join(' ',@slice), "\n"); } $self->flush if $self->_flush_on_write && defined $self->_fh; return 1; } 1; __END__