#----------------------------------------------------------------------------- # PACKAGE : Bio::SeqIO::tab # AUTHOR : Philip Lijnzaad # CREATED : Feb 6 2003 # REVISION: $Id: tab.pm 16123 2009-09-17 12:57:27Z cjfields $ # # Copyright (c) This module is free software; you can redistribute it # and/or modify it under the same terms as Perl itself. # # _History_ # # Ewan Birney developed the SeqIO # schema and the first prototype modules. # # This code is based on his Bio::SeqIO::raw # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code =head1 NAME Bio::SeqIO::tab - nearly raw sequence file input/output stream. Reads/writes id"\t"sequence"\n" =head1 SYNOPSIS Do not use this module directly. Use it via the L class. =head1 DESCRIPTION This object can transform Bio::Seq objects to and from tabbed flat file databases. It is very useful when doing large scale stuff using the Unix command line utilities (grep, sort, awk, sed, split, you name it). Imagine that you have a format converter 'seqconvert' along the following lines: my $in = Bio::SeqIO->newFh(-fh => \*STDIN , '-format' => $from); my $out = Bio::SeqIO->newFh(-fh=> \*STDOUT, '-format' => $to); print $out $_ while <$in>; then you can very easily filter sequence files for duplicates as: $ seqconvert < foo.fa -from fasta -to tab | sort -u |\ seqconvert -from tab -to fasta > foo-unique.fa Or grep [-v] for certain sequences with: $ seqconvert < foo.fa -from fasta -to tab | grep -v '^S[a-z]*control' |\ seqconvert -from tab -to fasta > foo-without-controls.fa Or chop up a huge file with sequences into smaller chunks with: $ seqconvert < all.fa -from fasta -to tab | split -l 10 - chunk- $ for i in chunk-*; do seqconvert -from tab -to fasta < $i > $i.fa; done # (this creates files chunk-aa.fa, chunk-ab.fa, ..., each containing 10 # sequences) =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.bioperl.org =head1 AUTHORS Philip Lijnzaad, p.lijnzaad@med.uu.nl =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::SeqIO::tab; use strict; use Bio::Seq; use base qw(Bio::SeqIO); =head2 next_seq Title : next_seq Usage : $seq = $stream->next_seq() Function: returns the next sequence in the stream Returns : Bio::Seq object Args : =cut sub next_seq{ my ($self,@args) = @_; ## When its 1 sequence per line with no formatting at all, ## grabbing it should be easy :) my $nextline = $self->_readline(); chomp($nextline) if defined $nextline; return unless defined $nextline; if ($nextline =~ /^([^\t]*)\t(.*)/) { my ($id, $seq)=($1, uc($2)); $seq =~ s/\W//g; return Bio::Seq->new(-display_id=> $id, -seq => $seq); } else { $self->throw("Can't parse tabbed sequence entry:'$nextline' around line $."); } } =head2 write_seq Title : write_seq Usage : $stream->write_seq($seq) Function: writes the $seq object into the stream Returns : 1 for success and 0 for error Args : Bio::Seq object =cut sub write_seq { my ($self,@seq) = @_; foreach (@seq) { if ($_->display_id() =~ /\t/) { $self->throw("display_id [".$_->display_id()."] contains TAB -- illegal in tab format"); } $self->_print($_->display_id(), "\t",$_->seq, "\n") or return; } $self->flush if $self->_flush_on_write && defined $self->_fh; return 1; } 1;