# $Id: table.pm 16123 2009-09-17 12:57:27Z cjfields $ # # BioPerl module for Bio::SeqIO::table # # Please direct questions and support issues to # # Cared for by Hilmar Lapp # # # (c) Hilmar Lapp, hlapp at gmx.net, 2005. # (c) GNF, Genomics Institute of the Novartis Research Foundation, 2005. # # You may distribute this module under the same terms as perl itself. # Refer to the Perl Artistic License (see the license accompanying this # software package, or see http://www.perl.com/language/misc/Artistic.html) # for the terms under which you may use, modify, and redistribute this module. # # THIS PACKAGE IS PROVIDED "AS IS" AND WITHOUT ANY EXPRESS OR IMPLIED # WARRANTIES, INCLUDING, WITHOUT LIMITATION, THE IMPLIED WARRANTIES OF # MERCHANTIBILITY AND FITNESS FOR A PARTICULAR PURPOSE. # # POD documentation - main docs before the code =head1 NAME Bio::SeqIO::table - sequence input/output stream from a delimited table =head1 SYNOPSIS #It is probably best not to use this object directly, but #rather go through the SeqIO handler system. Go: $stream = Bio::SeqIO->new(-file => $filename, -format => 'table'); while ( my $seq = $stream->next_seq() ) { # do something with $seq } =head1 DESCRIPTION This class transforms records in a table-formatted text file into Bio::Seq objects. A table-formatted text file of sequence records for the purposes of this module is defined as a text file with each row corresponding to a sequence, and the attributes of the sequence being in different columns. Columns are delimited by a common delimiter, for instance tab or comma. The module permits specifying which columns hold which type of annotation. The semantics of certain attributes, if present, are pre-defined, e.g., accession number and sequence. Additional attributes may be added to the annotation bundle. =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via email or the web: http://bugzilla.open-bio.org/ =head1 AUTHOR - Hilmar Lapp Email hlapp at gmx.net =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::SeqIO::table; use strict; use Bio::Species; use Bio::Seq::SeqFactory; use Bio::Annotation::Collection; use Bio::Annotation::SimpleValue; use base qw(Bio::SeqIO); =head2 new Title : new Usage : $stream = Bio::SeqIO->new(-file => $filename, -format => 'table') Function: Returns a new seqstream Returns : A Bio::SeqIO stream for a table format Args : Named parameters: -file name of file to read -fh filehandle to attach to -comment leading character(s) introducing a comment line -header the number of header lines to skip; the first non-comment header line will be used to obtain column names; column names will be used as the default tags for attaching annotation. -delim the delimiter for columns as a regular expression; consecutive occurrences of the delimiter will not be collapsed. -display_id the one-based index of the column containing the display ID of the sequence -accession_number the one-based index of the column containing the accession number of the sequence -seq the one-based index of the column containing the sequence string of the sequence -species the one-based index of the column containing the species for the sequence record; if not a number, will be used as the static species common to all records -annotation if provided and a scalar (but see below), a flag whether or not all additional columns are to be preserved as annotation, the tags used will either be 'colX' if there is no column header and where X is the one-based column index, and otherwise the column headers will be used as tags; if a reference to an array, or a square bracket-enclosed string of comma-delimited values, only those columns (one-based index) will be preserved as annotation, tags as before; if a reference to a hash, or a curly braces-enclosed string of comma-delimited key and value pairs in alternating order, the keys are one-based column indexes to be preserved, and the values are the tags under which the annotation is to be attached; if not provided or supplied as undef, no additional annotation will be preserved. -colnames a reference to an array of column labels, or a string of comma-delimited labels, denoting the columns to be converted into annotation; this is an alternative to -annotation and will be ignored if -annotation is also supplied with a valid value. -trim flag determining whether or not all values should be trimmed of leading and trailing white space and double quotes Additional arguments may be used to e.g. set factories and builders involved in the sequence object creation (see the POD of Bio::SeqIO). =cut sub _initialize { my($self,@args) = @_; # chained initialization $self->SUPER::_initialize(@args); # our own parameters my ($cmtchars, $header, $delim, $display_id, $accnr, $seq, $taxon, $useann, $colnames, $trim) = $self->_rearrange([qw(COMMENT HEADER DELIM DISPLAY_ID ACCESSION_NUMBER SEQ SPECIES ANNOTATION COLNAMES TRIM) ], @args); # store options and apply defaults $self->comment_char(defined($cmtchars) ? $cmtchars : "#") if (!defined($self->comment_char)) || defined($cmtchars); $self->delimiter(defined($delim) ? $delim : "\t") if (!defined($self->delimiter)) || defined($delim); $self->header($header) if defined($header); $self->trim_values($trim) if defined($trim); # attribute columns my $attrs = {}; $attrs->{-display_id} = $display_id if defined($display_id); $attrs->{-accession_number} = $accnr if defined($accnr); $attrs->{-seq} = $seq if defined($seq); if (defined($taxon)) { if (ref($taxon) || ($taxon =~ /^\d+$/)) { # either a static object, or a column reference $attrs->{-species} = $taxon; } else { # static species as a string $attrs->{-species} = Bio::Species->new( -classification => [reverse(split(' ',$taxon))]); } } $self->attribute_map($attrs); # annotation columns, if any if ($useann && !ref($useann)) { # it's a scalar; check whether this is in fact an array or # hash as a string rather than just a flag if ($useann =~ /^\[(.*)\]$/) { $useann = [split(/[,;]/,$1)]; } elsif ($useann =~ /^{(.*)}$/) { $useann = {split(/[,;]/,$1)}; } # else it is probably indeed just a flag } if (ref($useann)) { my $ann_map; if (ref($useann) eq "ARRAY") { my $has_header = ($self->header > 0); $ann_map = {}; foreach my $i (@$useann) { $ann_map->{$i} = $has_header ? undef : "col$i"; } } else { # no special handling necessary $ann_map = $useann; } $self->annotation_map($ann_map); } else { $self->keep_annotation($useann || $colnames); # annotation columns, if any if ($colnames && !ref($colnames)) { # an array as a string $colnames =~ s/^\[(.*)\]$/$1/; $colnames = [split(/[,;]/,$colnames)]; } $self->annotation_columns($colnames) if ref($colnames); } # make sure we have a factory defined if(!defined($self->sequence_factory)) { $self->sequence_factory( Bio::Seq::SeqFactory->new(-verbose => $self->verbose(), -type => 'Bio::Seq::RichSeq')); } } =head2 next_seq Title : next_seq Usage : $seq = $stream->next_seq() Function: returns the next sequence in the stream Returns : Bio::Seq::RichSeq object Args : =cut sub next_seq { my $self = shift; # skip until not a comment and not an empty line my $line_ok = $self->_next_record(); # if there is a header but we haven't read past it yet then do so now if ($line_ok && (! $self->_header_skipped) && $self->header) { $line_ok = $self->_parse_header(); $self->_header_skipped(1); } # return if we reached end-of-file return unless $line_ok; # otherwise, parse the record # split into columns my @cols = $self->_get_row_values(); # trim leading and trailing whitespace and quotes if desired if ($self->trim_values) { for(my $i = 0; $i < scalar(@cols); $i++) { if ($cols[$i]) { # trim off whitespace $cols[$i] =~ s/^\s+//; $cols[$i] =~ s/\s+$//; # trim off double quotes $cols[$i] =~ s/^"//; $cols[$i] =~ s/"$//; } } } # assign values for columns in the attribute map my $attrmap = $self->_attribute_map; my %params = (); foreach my $attr (keys %$attrmap) { if ((!ref($attrmap->{$attr})) && ($attrmap->{$attr} =~ /^\d+$/)) { # this is a column index, add to instantiation parameters $params{$attr} = $cols[$attrmap->{$attr}]; } else { # not a column index; we assume it's a static value $params{$attr} = $attrmap->{$attr}; } } # add annotation columns to the annotation bundle my $annmap = $self->_annotation_map; if ($annmap && %$annmap) { my $anncoll = Bio::Annotation::Collection->new(); foreach my $col (keys %$annmap) { next unless $cols[$col]; # skip empty columns! $anncoll->add_Annotation( Bio::Annotation::SimpleValue->new(-value => $cols[$col], -tagname=> $annmap->{$col})); } $params{'-annotation'} = $anncoll; } # ask the object builder to add the slots that we've gathered my $builder = $self->sequence_builder(); $builder->add_slot_value(%params); # and instantiate the object my $seq = $builder->make_object(); # done! return $seq; } =head2 comment_char Title : comment_char Usage : $obj->comment_char($newval) Function: Get/set the leading character(s) designating a line as a comment-line. Example : Returns : value of comment_char (a scalar) Args : on set, new value (a scalar or undef, optional) =cut sub comment_char{ my $self = shift; return $self->{'comment_char'} = shift if @_; return $self->{'comment_char'}; } =head2 header Title : header Usage : $obj->header($newval) Function: Get/set the number of header lines to skip before the rows containing actual sequence records. If set to zero or undef, means that there is no header and therefore also no column headers. Example : Returns : value of header (a scalar) Args : on set, new value (a scalar or undef, optional) =cut sub header{ my $self = shift; return $self->{'header'} = shift if @_; return $self->{'header'}; } =head2 delimiter Title : delimiter Usage : $obj->delimiter($newval) Function: Get/set the column delimiter. This will in fact be treated as a regular expression. Consecutive occurrences will not be collapsed to a single one. Example : Returns : value of delimiter (a scalar) Args : on set, new value (a scalar or undef, optional) =cut sub delimiter{ my $self = shift; return $self->{'delimiter'} = shift if @_; return $self->{'delimiter'}; } =head2 attribute_map Title : attribute_map Usage : $obj->attribute_map($newval) Function: Get/set the map of sequence object initialization attributes (keys) to one-based column index. Attributes will usually need to be prefixed by a dash, just as if they were passed to the new() method of the sequence class. Example : Returns : value of attribute_map (a reference to a hash) Args : on set, new value (a reference to a hash or undef, optional) =cut sub attribute_map{ my $self = shift; # internally we store zero-based maps - so we need to convert back # and forth here if (@_) { my $arg = shift; # allow for and protect against undef return delete $self->{'_attribute_map'} unless defined($arg); # copy to avoid side-effects my $attr_map = {%$arg}; foreach my $key (keys %$attr_map) { if ((!ref($attr_map->{$key})) && ($attr_map->{$key} =~ /^\d+$/)) { $attr_map->{$key}--; } } $self->{'_attribute_map'} = $attr_map; } # there may not be a map return unless exists($self->{'_attribute_map'}); # we need to copy in order not to override the stored map! my %attr_map = %{$self->{'_attribute_map'}}; foreach my $key (keys %attr_map) { if ((!ref($attr_map{$key})) && ($attr_map{$key} =~ /^\d+$/)) { $attr_map{$key}++; } } return \%attr_map; } =head2 annotation_map Title : annotation_map Usage : $obj->annotation_map($newval) Function: Get/set the mapping between one-based column indexes (keys) and annotation tags (values). Note that the map returned by this method may change after the first next_seq() call if the file contains a column header and no annotation keys have been predefined in the map, because upon reading the column header line the tag names will be set automatically. Note also that the map may reference columns that are used as well in the sequence attribute map. Example : Returns : value of annotation_map (a reference to a hash) Args : on set, new value (a reference to a hash or undef, optional) =cut sub annotation_map{ my $self = shift; # internally we store zero-based maps - so we need to convert back # and forth here if (@_) { my $arg = shift; # allow for and protect against undef return delete $self->{'_annotation_map'} unless defined($arg); # copy to avoid side-effects my $ann_map = {%$arg}; # make sure we sort the keys numerically or otherwise we may # clobber a key with a higher index foreach my $key (sort { $a <=> $b } keys(%$ann_map)) { $ann_map->{$key-1} = $ann_map->{$key}; delete $ann_map->{$key}; } $self->{'_annotation_map'} = $ann_map; # also make a note that we want to keep annotation $self->keep_annotation(1); } # there may not be a map return unless exists($self->{'_annotation_map'}); # we need to copy in order not to override the stored map! my %ann_map = %{$self->{'_annotation_map'}}; # here we need to sort numerically in reverse order ... foreach my $key (sort { $b <=> $a } keys(%ann_map)) { $ann_map{$key+1} = $ann_map{$key}; delete $ann_map{$key}; } return \%ann_map; } =head2 keep_annotation Title : keep_annotation Usage : $obj->keep_annotation($newval) Function: Get/set flag whether or not to keep values from additional columns as annotation. Additional columns are all those columns in the input file that aren't referenced in the attribute map. Example : Returns : value of keep_annotation (a scalar) Args : on set, new value (a scalar or undef, optional) =cut sub keep_annotation{ my $self = shift; return $self->{'keep_annotation'} = shift if @_; return $self->{'keep_annotation'}; } =head2 annotation_columns Title : annotation_columns Usage : $obj->annotation_columns($newval) Function: Get/set the names (labels) of the columns to be used for annotation. This is an alternative to using annotation_map. In order to have any effect, it must be set before the first call of next_seq(), and obviously there must be a header line (or row) too giving the column labels. Example : Returns : value of annotation_columns (a reference to an array) Args : on set, new value (a reference to an array of undef, optional) =cut sub annotation_columns{ my $self = shift; return $self->{'annotation_columns'} = shift if @_; return $self->{'annotation_columns'}; } =head2 trim_values Title : trim_values Usage : $obj->trim_values($newval) Function: Get/set whether or not to trim leading and trailing whitespace off all column values. Example : Returns : value of trim_values (a scalar) Args : on set, new value (a scalar or undef, optional) =cut sub trim_values{ my $self = shift; return $self->{'trim_values'} = shift if @_; return $self->{'trim_values'}; } =head1 Internal methods All methods with a leading underscore are not meant to be part of the 'official' API. They are for use by this module only, consider them private unless you are a developer trying to modify this module. =cut =head2 _attribute_map Title : _attribute_map Usage : $obj->_attribute_map($newval) Function: Get only. Same as attribute_map, but zero-based indexes. Note that any changes made to the returned map will change the map used by this instance. You should know what you are doing if you modify the returned value (or if you call this method in the first place). Example : Returns : value of _attribute_map (a reference to a hash) Args : none =cut sub _attribute_map{ my $self = shift; return $self->{'_attribute_map'}; } =head2 _annotation_map Title : _annotation_map Usage : $obj->_annotation_map($newval) Function: Get only. Same as annotation_map, but with zero-based indexes. Note that any changes made to the returned map will change the map used by this instance. You should know what you are doing if you modify the returned value (or if you call this method in the first place). Example : Returns : value of _annotation_map (a reference to a hash) Args : none =cut sub _annotation_map{ my $self = shift; return $self->{'_annotation_map'}; } =head2 _header_skipped Title : _header_skipped Usage : $obj->_header_skipped($newval) Function: Get/set the flag whether the header was already read (and skipped) or not. Example : Returns : value of _header_skipped (a scalar) Args : on set, new value (a scalar or undef, optional) =cut sub _header_skipped{ my $self = shift; return $self->{'_header_skipped'} = shift if @_; return $self->{'_header_skipped'}; } =head2 _next_record Title : _next_record Usage : Function: Navigates the underlying file to the next record. For row-based records in delimited text files, this will skip all empty lines and lines with a leading comment character. This method is here is to serve as a hook for other formats that conceptually also represent tables but aren't formatted as row-based text files. Example : Returns : TRUE if the navigation was successful and FALSE otherwise. Unsuccessful navigation will usually be treated as an end-of-file condition. Args : =cut sub _next_record{ my $self = shift; my $cmtcc = $self->comment_char; my $line = $self->_readline(); # skip until not a comment and not an empty line while (defined($line) && (($cmtcc && ($line =~ /^\s*$cmtcc/)) || ($line =~ /^\s*$/))) { $line = $self->_readline(); } return $self->{'_line'} = $line; } =head2 _parse_header Title : _parse_header Usage : Function: Parse the table header and navigate past it. This method is called if the number of header rows has been specified equal to or greater than one, and positioned at the first header line (row). By default the first header line (row) is used for setting column names, but additional lines (rows) may be skipped too. Empty lines and comment lines do not count as header lines (rows). This method will call _next_record() to navigate to the next header line (row), if there is more than one header line (row). Upon return, the file is presumed to be positioned at the first record after the header. This method is here is to serve as a hook for other formats that conceptually also represent tables but aren't formatted as row-based text files. Note however that the only methods used to access file content or navigate the position are _get_row_values() and _next_record(), so it should usually suffice to override those. Example : Returns : TRUE if navigation past the header was successful and FALSE otherwise. Unsuccessful navigation will usually be treated as an end-of-file condition. Args : =cut sub _parse_header{ my $self = shift; # the first header line contains the column headers, see whether # we need them if ($self->keep_annotation) { my @colnames = $self->_get_row_values(); # trim leading and trailing whitespace if desired if ($self->trim_values) { # trim off whitespace @colnames = map { $_ =~ s/^\s+//; $_ =~ s/\s+$//; $_; } @colnames; # trim off double quotes @colnames = map { $_ =~ s/^"//; $_ =~ s/"$//; $_; } @colnames; } # build or complete annotation column map my $annmap = $self->annotation_map || {}; if (! %$annmap) { # check whether columns have been defined by name rather than index if (my $anncols = $self->annotation_columns) { # first sanity check: all column names must map my %colmap = map { ($_,1); } @colnames; foreach my $col (@$anncols) { if (!exists($colmap{$col})) { $self->throw("no such column labeled '$col'"); } } # now map to the column indexes %colmap = map { ($_,1); } @$anncols; for (my $i = 0; $i < scalar(@colnames); $i++) { if (exists($colmap{$colnames[$i]})) { $annmap->{$i+1} = $colnames[$i]; } } } else { # no columns specified, default to all non-attribute columns for (my $i = 0; $i < scalar(@colnames); $i++) { $annmap->{$i+1} = $colnames[$i]; } # subtract all attribute-referenced columns foreach my $attrcol (values %{$self->attribute_map}) { if ((!ref($attrcol)) && ($attrcol =~ /^\d+$/)) { delete $annmap->{$attrcol}; } } } } else { # fill in where the tag names weren't pre-defined for (my $i = 0; $i < scalar(@colnames); $i++) { if (exists($annmap->{$i+1}) && ! defined($annmap->{$i+1})) { $annmap->{$i+1} = $colnames[$i]; } } } $self->annotation_map($annmap); } # now read past the header my $header_lines = $self->header; my $line_ok = 1; while (defined($line_ok) && ($header_lines > 0)) { $line_ok = $self->_next_record(); $header_lines--; } return $line_ok; } =head2 _get_row_values Title : _get_row_values Usage : Function: Get the values for the current line (or row) as an array in the order of columns. This method is here is to serve as a hook for other formats that conceptually also represent tables but aren't formatted as row-based text files. Example : Returns : An array of column values for the current row. Args : =cut sub _get_row_values{ my $self = shift; my $delim = $self->delimiter; my $line = $self->{'_line'}; chomp($line); my @cols = split(/$delim/,$line); return @cols; } 1;