# $Id: Palindrome.pm 16123 2009-09-17 12:57:27Z cjfields $ # # BioPerl module for Bio::Tools::EMBOSS::Palindrome # # Please direct questions and support issues to # # Cared for by Jason Stajich # # Copyright Jason Stajich # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code =head1 NAME Bio::Tools::EMBOSS::Palindrome - parse EMBOSS palindrome output =head1 SYNOPSIS # a simple script to turn palindrome output into GFF3 use Bio::Tools::EMBOSS::Palindrome; use Bio::Tools::GFF; my $parser = Bio::Tools::EMBOSS::Palindrome->new(-file => $filename); my $out = Bio::Tools::GFF->new(-gff_version => 3, -file => ">$filename.gff"); while( my $seq = $parser->next_seq ) { for my $feat ( $seq->get_SeqFeatures ) { $out->write_feature($feat); } } =head1 DESCRIPTION This is a parser for the EMBOSS tool 'palindrome'. It will produce a L object for each sequence analyzed. The sequence will be empty (but will be of the correct length) and will have attached to it L objects which wil =head2 FUTURE WORK It may be consolidated into another framework at a later time, but for the time being it will stay a separate modules. =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via email or the web: http://bugzilla.open-bio.org/ =head1 AUTHOR - Jason Stajich Email jason-at-bioperl-dot-org =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::Tools::EMBOSS::Palindrome; use vars qw($DEFAULT_SOURCETAG); use strict; use Bio::SeqFeature::FeaturePair; use Bio::SeqFeature::Generic; use base qw(Bio::Root::IO); $DEFAULT_SOURCETAG = 'palindrome'; =head2 new Title : new Usage : my $obj = Bio::Tools::EMBOSS::Palindrome->new(); Function: Builds a new Bio::Tools::EMBOSS::Palindrome object Returns : an instance of Bio::Tools::EMBOSS::Palindrome Args : -file/-fh => a filename or filehandle for initializing the parser =cut =head2 next_seq Title : next_seq Usage : my $seq = $parser->next_seq; Function: Get the next feature set from the Returns : L object Args : none =cut sub next_seq { my ($self) = @_; my (%searching, $seq,$state); my $source = $self->source_tag; $state = 0; while(defined($_ = $self->_readline)) { if( /^\s+$/ ) { next; } elsif( /^Palindromes\s+of\s*:\s+(\S+)/o ) { $state = 0; if( $seq ) { $self->_pushback($_); return $seq; } $seq = Bio::Seq->new(-display_id => $1); # now get ready to store for the next record $searching{'-seq_id'} = $1; } elsif( /^Sequence\s+length\s+is\s*:\s+(\d+)/o ) { $seq->length($1); $searching{'-tag'}->{'seqlength'} = $1; } elsif( /^(Start|End)\s+at\s+position\s*:\s+(\d+)/ ) { $searching{'-tag'}->{lc($1)} = $2; } elsif( m/^(Maximum|Minimum)\s+length\s+of\s+Palindromes\s+ is\s*:\s+(\d+)/ox) { $searching{'-tag'}->{lc($1).'_length'} = $2; } elsif( /^(Maximum\s+gap)\s+between\s+elements\s+is\s*:\s+(\d+)/o ) { $searching{'-tag'}->{lc($1)} = $2; } elsif( m/^Number\s+of\s+mismatches\s+allowed\s+ in\s+Palindrome\s*:\s+(\d+)/ox ) { $searching{'-tag'}->{'allowed_mismatches'} = $1; } elsif( /^Palindromes:/o ) { $state = 1; } elsif( $state == 1 ) { my $feature = Bio::SeqFeature::FeaturePair->new (-primary_tag => 'similarity', -source_tag => $source); for(my $i = 0; $i < 3; $i++ ) { if ($i != 1) { if( /^(\d+)\s+(\S+)\s+(\d+)/o ) { my ($start,$match,$end) = ($1,$2,$3); my $type = $i == 0 ? 'feature1' : 'feature2'; ($start,$end) = sort { $a <=> $b } ($start,$end); $feature->$type( Bio::SeqFeature::Generic->new (%searching, -start => $start, -end => $end, -strand => $i == 0 ? 1 : -1, -primary_tag => 'similarity', -source_tag => $source) ); } else { chomp; warn("Out of sync, line did not match:'$_'\n"); } } $_ = $self->_readline; } $seq->add_SeqFeature($feature); } } return $seq; } =head2 source_tag Title : source_tag Usage : $obj->source_tag($newval) Function: Get/Set Source Tag ('palindrome') by default Returns : value of source_tag (a scalar) Args : on set, new value (a scalar or undef, optional) =cut sub source_tag{ my $self = shift; return $self->{'source_tag'} = shift if @_; return $self->{'source_tag'} || $DEFAULT_SOURCETAG; } 1;