# $Id: FootPrinter.pm 16123 2009-09-17 12:57:27Z cjfields $ # BioPerl module for Bio::Tools::FootPrinter # # Please direct questions and support issues to # # Cared for by Shawn Hoon # # Copyright Shawn Hoon # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code =head1 NAME Bio::Tools::FootPrinter - write sequence features in FootPrinter format =head1 SYNOPSIS use Bio::Tools::FootPrinter; my $tool = Bio::Tools::FootPrinter->new(-file=>"footprinter.out"); while (my $result = $tool->next_feature){ foreach my $feat($result->sub_SeqFeature){ print $result->seq_id."\t".$feat->start."\t".$feat->end."\t".$feat->seq->seq."\n"; } } =head1 DESCRIPTION This module writes sequence features in FootPrinter format. See L for more details. =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHOR - Shawn Hoon Email shawnh@fugu-sg.org =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::Tools::FootPrinter; use strict; use Bio::SeqFeature::Generic; use Bio::PrimarySeq; use base qw(Bio::Root::Root Bio::Root::IO); =head2 new Title : new Usage : my $obj = Bio::Tools::FootPrinter->new(); Function: Builds a new Bio::Tools::FootPrinter object Returns : Bio::Tools::FootPrinter Args : -fh/-file => $val, # for initing input, see Bio::Root::IO =cut sub new { my($class,@args) = @_; my $self = $class->SUPER::new(@args); $self->_initialize_io(@args); return $self; } =head2 next_feature Title : next_feature Usage : my $r = $footprint->next_feature Function: Get the next feature from parser data Returns : L Args : none =cut sub next_feature{ my ($self) = @_; $self->_parse_predictions() unless $self->_predictions_parsed(); return shift @{$self->{'_feature'}}; } =head2 _add_feature Title : _add_feature Usage : $footprint->_add_feature($feat) Function: Add feature to array Returns : none Args : none =cut sub _add_feature { my ($self,$feat) = @_; if($feat){ push @{$self->{'_feature'}},$feat; } } =head2 _parse_predictions Title : _parse_predictions Usage : my $r = $footprint->_parse_predictions Function: do the parsing Returns : none Args : none =cut sub _parse_predictions { my ($self) = @_; $/=""; my ($seq,$second,$third,$name); while ($_ = $self->_readline) { chomp; my @array = split("\n",$_); if ($#array == 5) { # get rid of header shift(@array); shift(@array); } if($#array == 3){ if($name){ $name=~s/>//; my $feat = $self->_parse($name,$seq,$second,$third); $self->_add_feature($feat); } $name = shift @array; $seq = $array[0]; $second = $array[1]; $third = $array[2]; next; } $seq .= $array[0]; $third .= $array[2]; } $seq || return; $name=~s/>//; my $feat = $self->_parse($name,$seq,$second,$third); $self->_add_feature($feat); $self->_predictions_parsed(1); } =head2 _predictions_parsed Title : _predictions_parsed Usage : $footprint->_predictions_parsed(1) Function: Get/Set for whether predictions parsed Returns : 1/0 Args : none =cut sub _predictions_parsed { my ($self,$val) = @_; if($val){ $self->{'_predictions_parsed'} = $val; } return $self->{'_predictions_parsed'}; } =head2 _parse Title : _parse Usage : $footprint->_parse($name,$seq,$pattern) Function: do the actual parsing Returns : L Args : none =cut sub _parse { my ($self,$name,$seq,$score,$pattern) = @_; my @char = split('',$pattern); my @score = split('',$score); my ($prev,$word,@words,@word_scores,$word_score); my $i = 0; for my $c ( @char ) { if( ! $word) { $word .= $c; $prev = $c; defined $score[$i] && ($score[$i] =~ /\d/) && ($word_score += $score[$i]); } elsif ($c eq $prev){ $word .=$c; $prev = $c; defined $score[$i] && ($score[$i] =~ /\d/) && ($word_score += $score[$i]); } else { # remove words with only \s $word=~s/\s+//g; if ($word ne ''){ push @words, $word; push @word_scores, ($word_score/length($word)); } $word =$c; $prev = $c; $word_score = 0; defined $score[$i] && ($score[$i] =~ /\d/) && ($word_score += $score[$i]); } $i++; } $word =~s/\s+//g; if( length($word) ){ push @words, $word; } my $last; my $feat = Bio::SeqFeature::Generic->new(-seq_id=>$name); my $offset = $i = 0; my $count = 1; for my $w (@words){ if(length($w) ) { my $index = index($pattern,$w,$offset); $offset = $index + length($w); my $subfeat = Bio::SeqFeature::Generic->new ( -seq_id =>"$name-motif".$count++, -start => $index+1, -end => $index+length($w), -source =>"FootPrinter", -score => $word_scores[$i] ); # ugh - not sure the sub_SeqFeature situation will # be around forever- things should probably be # grouped by a 'group' tag instead ala GFF3 # perhaps when Lincoln's API changes are # made to SeqFeatures this will get changed $feat->add_sub_SeqFeature($subfeat,'EXPAND'); } $i++; } my $priseq = Bio::PrimarySeq->new(-id=>$name,-seq=>$seq); $feat->attach_seq($priseq); return $feat; } 1;