# $Id: Hmmpfam.pm 16123 2009-09-17 12:57:27Z cjfields $ # BioPerl module for Bio::Tools::Hmmpfam # # Please direct questions and support issues to # # Cared for by Balamurugan Kumarasamy # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code # =head1 NAME Bio::Tools::Hmmpfam - Parser for Hmmpfam program =head1 SYNOPSIS use Bio::Tools::Hmmpfam; my @hmmpfam_feat; my $hmmpfam_parser = Bio::Tools::Hmmpfam->new(-fh =>$filehandle ); while( my $hmmpfam_feat = $hmmpfam_parser->next_result ) { push @hmmpfam_feat, $hmmpfam_feat; } =head1 DESCRIPTION Parser for Hmmpfam program. See also L. =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHOR - Balamurugan Kumarasamy Email: fugui@worf.fugu-sg.org =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut package Bio::Tools::Hmmpfam; use strict; use Bio::SeqFeature::FeaturePair; use Bio::SeqFeature::Generic; use base qw(Bio::Root::Root Bio::Root::IO); =head2 new Title : new Usage : my $obj = Bio::Tools::Hmmpfam->new(-fh=>$filehandle); Function: Builds a new Bio::Tools::Hmmpfam object Returns : Bio::Tools::Hmmpfam Args : -filename -fh (filehandle) =cut sub new { my($class,@args) = @_; my $self = $class->SUPER::new(@args); $self->_initialize_io(@args); return $self; } =head2 next_result Title : next_result Usage : my $feat = $hmmpfam_parser->next_result Function: Get the next result set from parser data Returns : L Args : none =cut sub next_result { my ($self) = @_; my $filehandle; my $line; my $id; while ($_=$self->_readline()) { $line = $_; chomp $line; if ( $line=~m/^Alignments of top-scoring domains/ ) { while( my $rest = $self->_readline() ) { last if $rest =~ m!^//! } } next if ($line=~m/^Model/ || /^\-/ || /^$/); if ($line=~m/^Query sequence:\s+(\S+)/) { $id = $1; $self->seqname($id); } if (my ($hid, $start, $end, $hstart, $hend, $score, $evalue) = $line=~m/^(\S+)\s+\S+\s+(\d+)\s+(\d+)\s+\S+\s+(\d+)\s+(\d+)\s+\S+\s+(\S+)\s+(\S+)/) { my %feature; ($feature{name}) = $self->seqname; $feature{raw_score} = $score; $feature{p_value} = sprintf ("%.3e", $evalue); $feature{score} = $feature{p_value}; $feature{start} = $start; $feature{end} = $end; $feature{hname} = $hid; $feature{hstart} = $hstart; $feature{hend} = $hend; ($feature{source}) = 'pfam'; $feature{primary} = $hid; ($feature{program}) = 'pfam'; ($feature{db}) = 'db1'; ($feature{logic_name}) = 'hmmpfam'; my $new_feat = $self->create_feature (\%feature); return $new_feat } next; } return; } =head2 create_feature Title : create_feature Usage : my $feat=$hmmpfam_parser->create_feature($feature,$seqname) Function: creates a SeqFeature Generic object Returns : L Args : =cut sub create_feature { my ($self, $feat) = @_; my $feature1= Bio::SeqFeature::Generic->new( -seq_id =>$feat->{name}, -start =>$feat->{start}, -end =>$feat->{end}, -score =>$feat->{score}, -source =>$feat->{source}, -primary =>$feat->{primary}, ); my $feature2= Bio::SeqFeature::Generic->new( -start =>$feat->{hstart}, -end =>$feat->{hend}, ); my $featurepair = Bio::SeqFeature::FeaturePair->new; $featurepair->feature1 ($feature1); $featurepair->feature2 ($feature2); $featurepair->add_tag_value('evalue',$feat->{p_value}); $featurepair->add_tag_value('percent_id','NULL'); $featurepair->add_tag_value("hid",$feat->{primary}); return $featurepair; } =head2 seqname Title : seqname Usage : obj->seqname($seqname) Function: Internal(not to be used directly) Returns : Args : seqname =cut sub seqname{ my($self,$seqname)=@_; if(defined($seqname)) { $self->{'seqname'}=$seqname; } return $self->{'seqname'}; } 1;