# $Id: Seg.pm 16123 2009-09-17 12:57:27Z cjfields $ # # BioPerl module for Bio::Tools::Seg # # Copyright Balamurugan Kumarasamy # Totally re-written, added docs and tests -- Torsten Seemann, Sep 2006 # # Copyright # You may distribute this module under the same terms as perl itself # # POD documentation - main docs before the code =head1 NAME Bio::Tools::Seg - parse C output =head1 SYNOPSIS use Bio::Tools::Seg; my $parser = Bio::Tools::Seg->(-file => 'seg.fasta'); while ( my $f = $parser->next_result ) { if ($f->score < 1.5) { print $f->location->to_FTstring, " is low complexity\n"; } } =head1 DESCRIPTION C identifies low-complexity regions on a protein sequence. It is usually part of the C and C packages. The L module will only parse the "fasta" output modes of C, i.e. C (low complexity regions only), C (high complexity regions only), or C (both low and high). It creates a L for each FASTA-like entry found in the input file. It is up to the user to appropriately filter these using the feature's score. =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHOR - Torsten Seemann Email - torsten.seemann AT infotech.monash.edu.au =head1 CONTRIBUTOR - Bala Email - savikalpa@fugu-sg.org =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut package Bio::Tools::Seg; use strict; use Bio::SeqFeature::Generic; use base qw(Bio::Root::Root Bio::Root::IO); =head2 new Title : new Usage : my $obj = Bio::Tools::Seg->new(); Function: Builds a new Bio::Tools::Seg object Returns : Bio::Tools::Seg Args : -fh/-file => $val, # for initing input, see Bio::Root::IO =cut sub new { my($class,@args) = @_; my $self = $class->SUPER::new(@args); $self->_initialize_io(@args); return $self; } =head2 next_result Title : next_result Usage : my $feat = $seg->next_result Function: Get the next result set from parser data Returns : Bio::SeqFeature::Generic Args : none =cut sub next_result { my ($self) = @_; # For example in this line # test_prot(214-226) complexity=2.26 (12/2.20/2.50) # $1 is test_prot # $2 is 214 # $3 is 226 # $4 is 2.26 while (my $line = $self->_readline) { if ($line =~ /^\>\s*?(\S+)?\s*?\((\d+)\-(\d+)\)\s*complexity=(\S+)/) { return Bio::SeqFeature::Generic->new( -seq_id => $1, -start => $2, -end => $3, -score => $4, -source_tag => 'Seg', -primary => 'low_complexity' ); } } } 1;