# $Id: pag.pm 16123 2009-09-17 12:57:27Z cjfields $ # # BioPerl module for Bio::TreeIO::pag # # Please direct questions and support issues to # # Cared for by Jason Stajich # # Copyright Jason Stajich # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code =head1 NAME Bio::TreeIO::pag - Bio::TreeIO driver for Pagel format =head1 SYNOPSIS use Bio::TreeIO; my $in = Bio::TreeIO->new(-format => 'nexus', -file => 't/data/adh.mb_tree.nexus'); my $out = Bio::TreeIO->new(-format => 'pag'); while( my $tree = $in->next_tree ) { $out->write_tree($tree); } =head1 DESCRIPTION Convert a Bio::TreeIO to Pagel format. More information here http://www.evolution.reading.ac.uk/index.html =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.open-bio.org/ =head1 AUTHOR - Jason Stajich Email jason-at-bioperl-dot-org =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::TreeIO::pag; use strict; our $TaxonNameLen = 10; use base qw(Bio::TreeIO); =head2 new Title : new Usage : my $obj = Bio::TreeIO::pag->new(); Function: Builds a new Bio::TreeIO::pag object Returns : an instance of Bio::TreeIO::pag Args : -file/-fh for filename or filehandles -name_length for minimum name length (default = 10) =cut sub _initialize { my $self = shift; $self->SUPER::_initialize(@_); my ( $name_length ) = $self->_rearrange( [ qw(NAME_LENGTH) ], @_ ); $self->name_length( defined $name_length ? $name_length : $TaxonNameLen ); } =head2 write_tree Title : write_tree Usage : Function: Write a tree out in Pagel format Some options are only appropriate for bayesianmultistate and the simpler output is only proper for discrete Returns : none Args : -no_outgroups => (number) -print_header => 0/1 (leave 0 for discrete, 1 for bayesianms) -special_node => special node - not sure what they wanted to do here -keep_outgroup => 0/1 (keep the outgroup node in the output) -outgroup_ancestor => Bio::Tree::Node (if we want to exclude or include the outgroup this is what we operate on) -tree_no => a tree number label - only useful for BayesianMultistate =cut sub write_tree { my ($self,$tree,@args) = @_; my ($keep_outgroup, $print_header, $no_outgroups, $special_node, $outgroup_ancestor, $tree_no) = (0,0,1); my $name_len = $self->name_length; if( @args ) { ($no_outgroups, $print_header, $special_node, $outgroup_ancestor, $tree_no, $keep_outgroup) = $self->_rearrange([qw( NO_OUTGROUPS PRINT_HEADER SPECIAL_NODE OUTGROUP_ANCESTOR TREE_NO KEEP_OUTGROUP NAME_LENGTH)],@args); } my $newname_base = 1; my $root = $tree->get_root_node; my $eps = 0.0001; my (%chars,%names); my @nodes = $tree->get_nodes; my $species_ct; my $traitct; for my $node ( @nodes ) { if ((defined $special_node) && ($node eq $special_node)) { my $no_of_tree_nodes = scalar(@nodes); my $node_name = sprintf("N%d",$no_of_tree_nodes+1); $names{$node->internal_id} = $node_name; } elsif ($node->is_Leaf) { $species_ct++; my $node_name = $node->id; if( length($node_name)> $name_len ) { $self->warn( "Found a taxon name longer than $name_len letters, \n", "name will be abbreviated.\n"); $node_name = substr($node_name, 0,$name_len); } else { # $node_name = sprintf("%-".$TaxonNameLen."s",$node_name); } $names{$node->internal_id} = $node_name; my @tags = sort $node->get_all_tags; my @charstates = map { ($node->get_tag_values($_))[0] } @tags; $traitct = scalar @charstates unless defined $traitct; $chars{$node->internal_id} = [@charstates]; } else { $names{$node->internal_id} = sprintf("N%d", $newname_base++); } } # generate PAG representation if( $print_header ) { if ($keep_outgroup) { $self->_print(sprintf("%d %d\n",$species_ct,$traitct)); } else { $self->_print( sprintf("%d %d\n",$species_ct-$no_outgroups,$traitct)); } } my @ancestors = (); if ($keep_outgroup) { push @ancestors, $root; } else { push @ancestors, ( $root, $outgroup_ancestor); } my @rest; foreach my $node (@nodes) { my $i = 0; foreach my $anc (@ancestors) { if ($anc && $node eq $anc) { $i = 1; last } } unless ($i > 0) { # root not given in PAG my $current_name = $names{$node->internal_id}; my $branch_length_to_output; if ($node->branch_length < $eps) { my $msg_nodename = $current_name; $msg_nodename =~ s/\s+$//; warn( "TREE $tree_no, node \"$msg_nodename\": branch too ", "short (", $node->branch_length, "): increasing length to ", "$eps\n"); $branch_length_to_output = $eps; } else { $branch_length_to_output = $node->branch_length; } my @line = ( $current_name, $names{$node->ancestor->internal_id}, $branch_length_to_output); if ($node->is_Leaf) { push @line, @{$chars{$node->internal_id}}; $self->_print(join(',', @line),"\n"); } else { push @rest, \@line; } } } for ( @rest ) { $self->_print(join(',', @$_),"\n"); } } =head2 next_tree Title : next_tree Usage : Function: Example : Returns : Args : =cut sub next_tree{ my ($self,@args) = @_; $self->throw_not_implemented(); } =head2 name_length Title : name_length Usage : $self->name_length(20); Function: set mininum taxon name length Returns : integer (length of name) Args : integer =cut sub name_length { my ($self, $val) = @_; return $self->{'name_len'} = $val if $val; return $self->{'name_len'}; } 1;