#include "pysam.h" #include #include #include "bam.h" typedef struct { long long n_reads[2], n_mapped[2], n_pair_all[2], n_pair_map[2], n_pair_good[2]; long long n_sgltn[2], n_read1[2], n_read2[2]; long long n_dup[2]; long long n_diffchr[2], n_diffhigh[2]; } bam_flagstat_t; #define flagstat_loop(s, c) do { \ int w = ((c)->flag & BAM_FQCFAIL)? 1 : 0; \ ++(s)->n_reads[w]; \ if ((c)->flag & BAM_FPAIRED) { \ ++(s)->n_pair_all[w]; \ if ((c)->flag & BAM_FPROPER_PAIR) ++(s)->n_pair_good[w]; \ if ((c)->flag & BAM_FREAD1) ++(s)->n_read1[w]; \ if ((c)->flag & BAM_FREAD2) ++(s)->n_read2[w]; \ if (((c)->flag & BAM_FMUNMAP) && !((c)->flag & BAM_FUNMAP)) ++(s)->n_sgltn[w]; \ if (!((c)->flag & BAM_FUNMAP) && !((c)->flag & BAM_FMUNMAP)) { \ ++(s)->n_pair_map[w]; \ if ((c)->mtid != (c)->tid) { \ ++(s)->n_diffchr[w]; \ if ((c)->qual >= 5) ++(s)->n_diffhigh[w]; \ } \ } \ } \ if (!((c)->flag & BAM_FUNMAP)) ++(s)->n_mapped[w]; \ if ((c)->flag & BAM_FDUP) ++(s)->n_dup[w]; \ } while (0) bam_flagstat_t *bam_flagstat_core(bamFile fp) { bam_flagstat_t *s; bam1_t *b; bam1_core_t *c; int ret; s = (bam_flagstat_t*)calloc(1, sizeof(bam_flagstat_t)); b = bam_init1(); c = &b->core; while ((ret = bam_read1(fp, b)) >= 0) flagstat_loop(s, c); bam_destroy1(b); if (ret != -1) fprintf(pysamerr, "[bam_flagstat_core] Truncated file? Continue anyway.\n"); return s; } int bam_flagstat(int argc, char *argv[]) { bamFile fp; bam_header_t *header; bam_flagstat_t *s; if (argc == optind) { fprintf(pysamerr, "Usage: samtools flagstat \n"); return 1; } fp = strcmp(argv[optind], "-")? bam_open(argv[optind], "r") : bam_dopen(fileno(stdin), "r"); assert(fp); header = bam_header_read(fp); s = bam_flagstat_core(fp); printf("%lld + %lld in total (QC-passed reads + QC-failed reads)\n", s->n_reads[0], s->n_reads[1]); printf("%lld + %lld duplicates\n", s->n_dup[0], s->n_dup[1]); printf("%lld + %lld mapped (%.2f%%:%.2f%%)\n", s->n_mapped[0], s->n_mapped[1], (float)s->n_mapped[0] / s->n_reads[0] * 100.0, (float)s->n_mapped[1] / s->n_reads[1] * 100.0); printf("%lld + %lld paired in sequencing\n", s->n_pair_all[0], s->n_pair_all[1]); printf("%lld + %lld read1\n", s->n_read1[0], s->n_read1[1]); printf("%lld + %lld read2\n", s->n_read2[0], s->n_read2[1]); printf("%lld + %lld properly paired (%.2f%%:%.2f%%)\n", s->n_pair_good[0], s->n_pair_good[1], (float)s->n_pair_good[0] / s->n_pair_all[0] * 100.0, (float)s->n_pair_good[1] / s->n_pair_all[1] * 100.0); printf("%lld + %lld with itself and mate mapped\n", s->n_pair_map[0], s->n_pair_map[1]); printf("%lld + %lld singletons (%.2f%%:%.2f%%)\n", s->n_sgltn[0], s->n_sgltn[1], (float)s->n_sgltn[0] / s->n_pair_all[0] * 100.0, (float)s->n_sgltn[1] / s->n_pair_all[1] * 100.0); printf("%lld + %lld with mate mapped to a different chr\n", s->n_diffchr[0], s->n_diffchr[1]); printf("%lld + %lld with mate mapped to a different chr (mapQ>=5)\n", s->n_diffhigh[0], s->n_diffhigh[1]); free(s); bam_header_destroy(header); bam_close(fp); return 0; }