#include "pysam.h" #include #include #include #include #include "bcf.h" static int8_t nt4_table[256] = { 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4 /*'-'*/, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 0, 4, 1, 4, 4, 4, 2, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 3, 4, 4, 4, -1, 4, 4, 4, 4, 4, 4, 4, 4, 0, 4, 1, 4, 4, 4, 2, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 3, 4, 4, 4, -1, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4 }; static int read_I16(bcf1_t *b, int anno[16]) { char *p; int i; if ((p = strstr(b->info, "I16=")) == 0) return -1; p += 4; for (i = 0; i < 16; ++i) { anno[i] = strtol(p, &p, 10); if (anno[i] == 0 && (errno == EINVAL || errno == ERANGE)) return -2; ++p; } return 0; } int bcf_2qcall(bcf_hdr_t *h, bcf1_t *b) { int a[4], k, g[10], l, map[4], k1, j, i, i0, anno[16], dp, mq, d_rest; char *s; if (b->ref[1] != 0 || b->n_alleles > 4) return -1; // ref is not a single base for (i = 0; i < b->n_gi; ++i) if (b->gi[i].fmt == bcf_str2int("PL", 2)) break; if (i == b->n_gi) return -1; // no PL if (read_I16(b, anno) != 0) return -1; // no I16; FIXME: can be improved d_rest = dp = anno[0] + anno[1] + anno[2] + anno[3]; if (dp == 0) return -1; // depth is zero mq = (int)(sqrt((double)(anno[9] + anno[11]) / dp) + .499); i0 = i; a[0] = nt4_table[(int)b->ref[0]]; if (a[0] > 3) return -1; // ref is not A/C/G/T a[1] = a[2] = a[3] = -2; // -1 has a special meaning if (b->alt[0] == 0) return -1; // no alternate allele map[0] = map[1] = map[2] = map[3] = -2; map[a[0]] = 0; for (k = 0, s = b->alt, k1 = -1; k < 3 && *s; ++k, s += 2) { if (s[1] != ',' && s[1] != 0) return -1; // ALT is not single base a[k+1] = nt4_table[(int)*s]; if (a[k+1] >= 0) map[a[k+1]] = k+1; else k1 = k+1; if (s[1] == 0) break; } for (k = 0; k < 4; ++k) if (map[k] < 0) map[k] = k1; for (i = 0; i < h->n_smpl; ++i) { int d; uint8_t *p = b->gi[i0].data + i * b->gi[i0].len; for (j = 0; j < b->gi[i0].len; ++j) if (p[j]) break; d = (int)((double)d_rest / (h->n_smpl - i) + .499); if (d == 0) d = 1; if (j == b->gi[i0].len) d = 0; d_rest -= d; for (k = j = 0; k < 4; ++k) { for (l = k; l < 4; ++l) { int t, x = map[k], y = map[l]; if (x > y) t = x, x = y, y = t; // swap g[j++] = p[y * (y+1) / 2 + x]; } } printf("%s\t%d\t%c", h->ns[b->tid], b->pos+1, *b->ref); printf("\t%d\t%d\t0", d, mq); for (j = 0; j < 10; ++j) printf("\t%d", g[j]); printf("\t%s\n", h->sns[i]); } return 0; }