Primer3 Output
No mispriming library specified
Using 1-based sequence positions
OLIGO start len tm gc% any 3' seq
LEFT PRIMER 41 20 59.15 45.00 5.00 3.00 TTTTCCAGCTCATGCTGAAG
RIGHT PRIMER 100 21 58.06 42.86 5.00 0.00 GCCTTTGCCTTAACTGTTTTC
SEQUENCE SIZE: 103
INCLUDED REGION SIZE: 103
PRODUCT SIZE: 60, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
1 TAGCCCCTTTTCTAGGAGGCTCTCCTCCTGGGCTGGGGCCTTTTCCAGCTCATGCTGAAG
>>>>>>>>>>>>>>>>>>>>
61 TCTTTCAAACCTGGGAGAAGAAAACAGTTAAGGCAAAGGCctg
<<<<<<<<<<<<<<<<<<<<<
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len tm gc% any 3' seq
1 LEFT PRIMER 43 20 59.12 50.00 5.00 2.00 TTCCAGCTCATGCTGAAGTC
RIGHT PRIMER 100 21 58.06 42.86 5.00 0.00 GCCTTTGCCTTAACTGTTTTC
PRODUCT SIZE: 58, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
2 LEFT PRIMER 40 21 60.14 47.62 5.00 3.00 CTTTTCCAGCTCATGCTGAAG
RIGHT PRIMER 100 21 58.06 42.86 5.00 0.00 GCCTTTGCCTTAACTGTTTTC
PRODUCT SIZE: 61, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
3 LEFT PRIMER 41 20 59.15 45.00 5.00 3.00 TTTTCCAGCTCATGCTGAAG
RIGHT PRIMER 101 22 61.29 45.45 5.00 0.00 gGCCTTTGCCTTAACTGTTTTC
PRODUCT SIZE: 61, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
4 LEFT PRIMER 43 20 59.12 50.00 5.00 2.00 TTCCAGCTCATGCTGAAGTC
RIGHT PRIMER 101 22 61.29 45.45 5.00 0.00 gGCCTTTGCCTTAACTGTTTTC
PRODUCT SIZE: 59, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
Statistics
con too in in no tm tm high high high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab ok
Left 279 0 0 0 0 27 31 181 0 12 0 8 20
Right 258 0 0 0 0 30 68 98 0 0 0 8 54
Pair Stats:
considered 441, unacceptable product size 405, high end compl 8, ok 28
primer3 release 0.9
(primer3_www_results.cgi v 0.1 beta 1a)