Primer3 Output
No mispriming library specified
Using 1-based sequence positions
OLIGO start len tm gc% any 3' seq
LEFT PRIMER 94 21 60.27 42.86 3.00 0.00 TGGAAAACAGAGCCACAAAAG
RIGHT PRIMER 494 20 60.07 55.00 6.00 2.00 TTCCTGGGAGGTACATCTGC
SEQUENCE SIZE: 563
INCLUDED REGION SIZE: 563
PRODUCT SIZE: 401, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
1 TCACGAACTGATTGGCAGCTTTTAATAGCGATGCCATCGATGAATGCCAATGAAATGCCA
61 ACACTTGTTGGCGTATGCCAACCTGAAATTTCATGGAAAACAGAGCCACAAAAGAGAAAC
>>>>>>>>>>>>>>>>>>>>>
121 AATGGCTGTGTCTGGTAGGTGGGATTGTTAGCGATTTTTGCTTTCAAATTATTATATGGG
181 AACAGCCACTGCTTTTGCAATGGTATTTTTAAGGACCTCTGTTGTACCACACTCTACTTG
241 AGCTTGATACTTGAGCCTTTTGTGAGTTTTGATTCTGCTTTGTTTTTCTTCCCTTCCTTT
301 CTTTCTCTAGCTCTCAACTCAGATTTCTTTGTAGACTGGTTTAATTACTATCTAGAAACA
361 GCATACGTATTTAGACTGGAGCATAGCCCTGGCAGCCTAGTTCTCACTATCTGAGAGACC
421 CACGGAGAGCATCTGGCCTGCCCCGATTGGCAGGGGGAGTTGGTAAAACTCCCTGCAGAT
<<<<<<
481 GTACCTCCCAGGAAGCCAGTCTTCAGGGGTCAGGCCCAAGGAGGCATTTTTCAGCAGCCT
<<<<<<<<<<<<<<
541 TAAAGGGCTGCCTTCAAAACCTG
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len tm gc% any 3' seq
1 LEFT PRIMER 105 21 59.60 42.86 3.00 2.00 GCCACAAAAGAGAAACAATGG
RIGHT PRIMER 494 20 60.07 55.00 6.00 2.00 TTCCTGGGAGGTACATCTGC
PRODUCT SIZE: 390, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
2 LEFT PRIMER 38 20 59.89 40.00 3.00 2.00 CGATGAATGCCAATGAAATG
RIGHT PRIMER 425 21 59.73 57.14 5.00 1.00 CCGTGGGTCTCTCAGATAGTG
PRODUCT SIZE: 388, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
3 LEFT PRIMER 116 20 59.14 50.00 4.00 0.00 GAAACAATGGCTGTGTCTGG
RIGHT PRIMER 494 20 60.07 55.00 6.00 2.00 TTCCTGGGAGGTACATCTGC
PRODUCT SIZE: 379, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
4 LEFT PRIMER 38 20 59.89 40.00 3.00 2.00 CGATGAATGCCAATGAAATG
RIGHT PRIMER 434 20 61.76 60.00 4.00 1.00 AGATGCTCTCCGTGGGTCTC
PRODUCT SIZE: 397, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
Statistics
con too in in no tm tm high high high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab ok
Left 1551 0 0 0 46 226 587 291 12 6 0 23 360
Right 1597 0 0 0 3 200 530 520 0 12 0 41 291
Pair Stats:
considered 83, unacceptable product size 38, high end compl 1, ok 44
primer3 release 0.9
(primer3_www_results.cgi v 0.1 beta 1a)