Primer3 Output
No mispriming library specified
Using 1-based sequence positions
OLIGO start len tm gc% any 3' seq
LEFT PRIMER 173 20 59.60 55.00 4.00 0.00 AGACTTCTGCTCAGGCCAAC
RIGHT PRIMER 420 20 60.37 50.00 4.00 2.00 TTGAGGCAGACCCTTTTCTG
SEQUENCE SIZE: 656
INCLUDED REGION SIZE: 656
PRODUCT SIZE: 248, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
1 CTTTGTTGAATCCACAAATCCTGGCTTTGTCTGAGTGTGTGTGTGAGAAGGGGGGTCTGA
61 AAATAATTCCACAGCCAGCTACCACTGTGGTGTGGGTCTGTTTTCCCATCATTAGTGACA
121 ACCCAACTAGATCAGACACTGCAGGAAGAGGAAACGTAGAAGAACCCAGACCAGACTTCT
>>>>>>>>
181 GCTCAGGCCAACATACCTGCAGCAGCCCCTCATCTCTCATGGTCACTGTAAGAAATAAAT
>>>>>>>>>>>>
241 TGCTGTGGTCCATTTTCAGTGTGCTCTGAACTTAGAGATCAAAGAATCACATATTGAAAC
301 CAGGAGGCCCAACCCCTGCCCCAATCTCCTCTAATTGGTGGCCAGCAGTTTGCAGAACTG
361 CAAAGTTACAGGCAGTCTTCCTTGTGTCTGAAGCTGCAAACAGAAAAGGGTCTGCCTCAA
<<<<<<<<<<<<<<<<<<<<
421 CCATGAATATTCCTTAGATGAGACGTTGCTTCTGAAATAATCTGATGGGGGGAATTTTGN
481 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
541 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
601 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len tm gc% any 3' seq
1 LEFT PRIMER 173 20 59.60 55.00 4.00 0.00 AGACTTCTGCTCAGGCCAAC
RIGHT PRIMER 416 20 59.71 50.00 4.00 0.00 GGCAGACCCTTTTCTGTTTG
PRODUCT SIZE: 244, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00
2 LEFT PRIMER 87 20 60.26 55.00 2.00 0.00 GTGGTGTGGGTCTGTTTTCC
RIGHT PRIMER 345 20 59.69 55.00 6.00 2.00 CTGGCCACCAATTAGAGGAG
PRODUCT SIZE: 259, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
3 LEFT PRIMER 66 20 60.14 55.00 4.00 0.00 ATTCCACAGCCAGCTACCAC
RIGHT PRIMER 309 20 59.39 50.00 4.00 2.00 GGCCTCCTGGTTTCAATATG
PRODUCT SIZE: 244, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
4 LEFT PRIMER 177 20 58.88 50.00 4.00 0.00 TTCTGCTCAGGCCAACATAC
RIGHT PRIMER 428 20 59.93 50.00 4.00 3.00 ATTCATGGTTGAGGCAGACC
PRODUCT SIZE: 252, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00
Statistics
con too in in no tm tm high high high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab ok
Left 2116 104 0 0 0 245 581 661 9 13 10 45 448
Right 1899 177 0 0 0 206 512 564 2 3 5 32 398
Pair Stats:
considered 265, unacceptable product size 131, high end compl 16, ok 118
primer3 release 0.9
(primer3_www_results.cgi v 0.1 beta 1a)